Immunization with outer membrane protein P6 from nontypeable Haemophilus influenzae induces bactericidal antibody and affords protection in the chinchilla model of otitis media

Infection and Immunity

Volumn 64, Issue 12, 1996, Pages 5187-5192

  DeMaria, T F ^a   Murwin, Debra M ^a   Leake, Edward R ^a  

^a THE OHIO STATE UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM ANTIBODY; HAEMOPHILUS VACCINE; OUTER MEMBRANE PROTEIN;

ACTIVE IMMUNIZATION; ANIMAL MODEL; ARTICLE; BACTERIAL COLONIZATION; BACTERICIDAL ACTIVITY; CHINCHILLA; CONTROLLED STUDY; HAEMOPHILUS INFLUENZAE; INFECTION PREVENTION; NONHUMAN; OTITIS MEDIA; PRIORITY JOURNAL; SUBCUTANEOUS DRUG ADMINISTRATION;

EID: 0029846855     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.12.5187-5192.1996     Document Type: Article

References (34)

1. Andersen, P., A. Fiesher, S. Shaw, A. L. Harding, and D. H. Smith. 1980. Phenotypic and genetic variation in the susceptibility of Haemophilia influenzae type b to antibodies to somatic antigens. J. Clin. Invest. 65:885-891.
2. Bakaleb, L. O., T. M. Hoepf, D. J. Lim, and B. Tallan. 1990. Colonization of the chinchilla middle car und nasopharynx by fimbriated isolates of nontypable Haemophilia influenzae, abstr. B-66, p. 37. In Abstracts of the 1990 Annual Meeting of the American Society for Microbiology 1990. American Society for Microbiology. Washington, D.C.
3. Bakaleti, L. O., B. M. Tallan, W. J. Andrzejewski, T. F. DeMaria, and D. J. Lim. 1989. Immunological responsiveness of chinchillas to outer membrane and isolated fimbrial proteins of nontypeable Haemophilia influenzae. Infect. Immun. 57:3226-3229.
4. Barenkamp, S. J. 1986. Protection by serum antibodies in experimental nontypeablc Haemophilia influenzae otitis media. Infect. Immun. 52:572-580.
5. Clancy, R. L. and A. W. Cripps. 1992. Specific protection against acute bronchitis associated with nontypeable Hatmopliilus influenzae. J. Infect. Dis. 165(Suppl. 1):S194-S195.
6. Deich, R. M., A. Anilionls, J. Fulginiti, B. J. Metcalf, S. Quataert, T. Quinn-Dey, G. W. Zlotnick, and B. A. Green. 1990. Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera. Infect. Immun. 58:3388-3393.
7. DeMaria, T. F. 1990. Animal models for nontypable Haemophilia influenzae otitis media. Pediatr. Infect. Dis. J. 8:S40-S42.
8. DeMaria, T. F., B. R. Briggs, N. Okazaki, and D. J. Lim. 1984. Experimental otitis media with effusion following middle ear inoculation of nonviable H. influenzae. Ann. Otol. Rhinol. Laryngol. 93:51-56.
9. Green, B. A., J. E. Farley, T. Quinn-Dev, R. A. Deich, and G. W. Zlotnick. 1991. e (P4) outer membrane protein of Haemaphilus influenzae: biologic activity of anti-e serum and cloning and sequencing of the structural gene. Infect. Immun. 58:3191-3198.
10. Green, B. A., B. J. Metcalf, T. Quinn-Dey, D. H. Kirkley, S. A. Quataert, and R. A. Deich. 1990. A recombinant non-fatty acyluted form of the Hi-PAL (P6) protein of Haemopliilus influenzae elicits biologically active antibody against both nontypeable and type b H. influenzae. Infect. Immun. 58:3272-3278.
11. Green, B. A., T. Quinn-Dey, and G. W. Zlotnick. 1987. Biologic activities of antibody to a peptidoglycan-associate lipoprotein of Haemophilia influenzae against multiple clinical isolates of H. influenza? type b. Infect. Immun. 55:2878-2883.
12. Green, B. A., M. E. Vazquez, G. W. Zlotnick, G. Qulgley-Reape, J. D. Swarts, I. Green, J. L. Colwell, C. D. Bluestone, and W. J. Doyle. 1993. Evaluation of mixtures of purified Haemophilus inftuenzae outer membrane proteins in protection against challenge with nontypeable H. influenzae in the chinchilla otitis media model. Infect. Immun. 61:1950-1957.
13. Groeneveld, K., P. P. Eljk, L. van Alphen, H. M. Jansen, and H. C. Zanen. 1990B. Haemophilia influenzae infections in patients with chronic obstructive pulmonary disease despite specific antibodies in serum and sputum. Am. Rev. Respir. Dis. 141:1316-1321.
14. Groeneveld, K., L. van Alphen, C. Voorter, P. P. Eijk, H. M. Jansen, and H. C. Zanen. 1989. Antigenic drift of Haemophilia influenzae in patients with chronic obstructive pulmonary disease. Infect. Immun. 57:3038-3044.
15. Hansen, E. J., D. A. Hart, L. McGhee, and G. B. Toews. 1988. Immune enhancement of pulmonary clearance of nontypeable Haemophilia influenzae. Infect. Immun. 56:182-190.
16. Karasic, R. B., D. J. Beste, S. C. To, W. J. Doyle, S. W. Wood, M. J. Carter, A. C. To, K. Tanpowpong, C. D. Bluestone, and C. C. Brinton, Jr. 1989. Evaluation of pilus vaccines for prevention of experimental otitis media caused hy nontypable Haemophilus Influenzae. Pediatr. Infect. Dis. J. 8 (Suppl. 1):S62-S65.
17. Kyd, J. M., M. L. Dunkley, and A. W. Cripps. 1995. Enhanced respiratory clearance of nontypeable Haemophilia influenzae following mucosul immunization with Pb in a rat model. Infect. Immun. 63:2931-2940.
18. Leake, E. R., K. Holmes, D. J. Lim, and T. F. UeMaria. 1994. Peptidoglycan isolated from nontypeable Haemophilus influenzae induces experimental otitis media in the chinchilla. J. Infect. Dis. 170:1532-1538.
19. Llu, V. C., and A. L. Smith. 1992. Molecular mechanisms of Haemophilia influenzae pathogenicity. Antibiot. Chemother, 45:30-51.
20. Munson, R. S., and D. M. Granoff. 1985. Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b. Infect. Immun. 49:544-549.
21. Murphy, T. F. (SUNY Buffalo). 1995. Personal communication.
22. Murphy, T. F., and M. A. Apicella. 1987. Nontypable Haemophilus influenzae: a review of clinical aspects, surface antigens, and the human immune response to infection. Rev. Infect. Dis. 9:1-15.
23. Murphy, T. F., L. C. Bartos, A. A. Campagnari, M. B. Nelson, and M. A. Aplcella. 1986. Antigenic characterization of the P6 protein of nontypeable Haemophilia influenzae. Infect. Immun. 54:774-779.
24. Murphy, T. F., L. C. Bartos, P. A. Rice, M. B. Nelson, K. U. Dudas, and M. A. Apicella. 1986. Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody. J. Clin. Invest. 78:1020-1027.
25. Murphy, T. F., A. A. Campagnari, M. B. Nelson, and M. A. Apicella. 1989. Somatic antigens of Haemophilus influenzae as vaccine components. Pediatr. Infect. Dis. J. 8:S66-S68.
26. Murphy, T. F., C. Kirkham, and D. J. Sikkema. 1992. Neonatal, urogenital isolates of biotype 4 nontypeable Haemophilus influenzae express a variant P6 outer membrane protein molecule. Infect. Immun. 60:2016-2022.
27. Murphy, T. F., M. B. Nelson, K. C. Dudas, J. M. Mylotte, and M. A. Apicella. 1985. Identification of a specific epitoipe of Haemophilus influenzae on a 16,600 dalton outer membrane protein. J. Infect. Dis. 152:13100-1307.
28. Rosenfeld, R. M., W. J. Doyle, D. Swarts, J. Seroky, and B. P. Pinero. 1992. Third-generation cephalosporins in the treatment of acute pneumococcal otitis media. An animal study. Arch. Otolaryngol. Head Neck Surg. 118:49-52.
29. Sirakova, T., P. E. Kolattukudy, D. Murwin, J. Billy, E. Leake, D. J. Lim, T. F. DeMaria, and L. O. Bakaletz. 1994. The role of fimbriae expressed by nontypcable Haemophilus influemaze in the pathogenesis of and protection against otitis media and the relatedness of the fimbrin subunit to outer membrane protein A. Infect. Immun. 62:2002-2020.
30. Stjernquist-Desatnik, A., K. Prellner, and C. Schalen. 1990. Colonization by Haemophilus influenzae and group A Streptococci in recurrent acute tonsillitis and in tonsillar hypertrophy. Acta Otolaryngol. (Stockholm) 109:314-319.
31. Suzuki, K., and L. O. Bakaletz. 1994. Synergistic effect of adenovirus type 1 and nontypeablc Haemophilus influenzae in a chinchilla model of experimental otitis media. Infect. Immun. 62:1710-1718.
32. Tsai, C. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamidc gels. Anal. Biochem. 119:115-119.
33. Wald, E. R., C. Byers, N. Guerra, M. Casselbrant, and D. Beste. 1989. Subacute sinusitis in children. J. Pediatr. 115:28-32.
34. Yamanaka, N., and H. Faden. 1993. Local antibody response to P6 of nontypable Haemanhilus influenzae in otitis-prone and normal children. Acta Otolaryngol. (Stockholm) 113:524-529.