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1
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85030364013
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Ergebnisse über die Konstitution der chromatischen Substanz des Zellkerns
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[Title translation:
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Boveri T: Ergebnisse über die Konstitution der chromatischen Substanz des Zellkerns. Jena G Fisher 1904. [Title translation: Results on the constitution of the chromatic substance of the nuclei.].
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(1904)
Jena G Fisher
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Boveri, T.1
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2
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0002852643
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Zellenstudien VI: Die Entwicklung dispermer Seeigeleier. Ein Beitrag zur Befruchtungslehre und zur Theorie des Kernes
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[Title translation: Cell studies VI - Development of dispermic sea urchin eggs. Contribution to the fertilization theory and the theory of the nucleus.]
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Boveri T Zellenstudien VI: die Entwicklung dispermer Seeigeleier. Ein Beitrag zur Befruchtungslehre und zur Theorie des Kernes. Jena Zeitschr Naturw. 43:1907;1-292. [Title translation: Cell studies VI - Development of dispermic sea urchin eggs. Contribution to the fertilization theory and the theory of the nucleus.].
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(1907)
Jena Zeitschr Naturw
, vol.43
, pp. 1-292
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Boveri, T.1
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3
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0031697442
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Specification of cell fate in the sea urchin embryo: Summary and some proposed mechanisms
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A recent review providing an interpretive overview of cell lineage, intercellular signaling and maternal cytoarchitectural inputs in the specification of the major territories of the early embryo; regulatory aspects of postembryonic development are also considered. The review includes an update and revision of my 1989 model for sea urchin embryogenesis.
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Davidson EH, Cameron RA, Ransick A Specification of cell fate in the sea urchin embryo: summary and some proposed mechanisms. Development. 125:1998;3269-3290. A recent review providing an interpretive overview of cell lineage, intercellular signaling and maternal cytoarchitectural inputs in the specification of the major territories of the early embryo; regulatory aspects of postembryonic development are also considered. The review includes an update and revision of my 1989 model for sea urchin embryogenesis.
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(1998)
Development
, vol.125
, pp. 3269-3290
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Davidson, E.H.1
Cameron, R.A.2
Ransick, A.3
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5
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0024085757
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Developmental appearance of factors that bind specifically to cis-regulatory sequences of a gene expressed in the sea urchin embryo
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Calzone FJ, Thézé N, Thiebaud P, Hill RL, Britten RJ, Davidson EH Developmental appearance of factors that bind specifically to cis-regulatory sequences of a gene expressed in the sea urchin embryo. Genes Dev. 2:1998;1074-1088.
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(1998)
Genes Dev
, vol.2
, pp. 1074-1088
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Calzone, F.J.1
Thézé, N.2
Thiebaud, P.3
Hill, R.L.4
Britten, R.J.5
Davidson, E.H.6
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6
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0025100558
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Competitive titration in living sea urchin embryos of regulatory factors required for expression of the CyIIIa actin gene
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Franks RR, Anderson R, Moore JG, Hough-Evans BR, Britten RJ, Davidson EH Competitive titration in living sea urchin embryos of regulatory factors required for expression of the CyIIIa actin gene. Development. 110:1990;31-40.
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(1990)
Development
, vol.110
, pp. 31-40
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Franks, R.R.1
Anderson, R.2
Moore, J.G.3
Hough-Evans, B.R.4
Britten, R.J.5
Davidson, E.H.6
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7
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0025100559
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Negative spatial regulation of the lineage specific CyIIIa actin gene in the sea urchin embryo
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Hough-Evans BR, Franks RR, Zeller RW, Britten RJ, Davidson EH Negative spatial regulation of the lineage specific CyIIIa actin gene in the sea urchin embryo. Development. 110:1990;41-50.
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(1990)
Development
, vol.110
, pp. 41-50
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Hough-Evans, B.R.1
Franks, R.R.2
Zeller, R.W.3
Britten, R.J.4
Davidson, E.H.5
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8
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0025264293
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Sequences of the CyIIIa actin gene regulatory domain bound specifically by sea urchin embryo nuclear proteins
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Thézé N, Calzone FJ, Thiebaud P, Hill RL, Britten RJ, Davidson EH Sequences of the CyIIIa actin gene regulatory domain bound specifically by sea urchin embryo nuclear proteins. Mol Reprod Dev. 25:1990;110-122.
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(1990)
Mol Reprod Dev
, vol.25
, pp. 110-122
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Thézé, N.1
Calzone, F.J.2
Thiebaud, P.3
Hill, R.L.4
Britten, R.J.5
Davidson, E.H.6
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9
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0025998888
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Differential stability of expression of similarly specified endogenous and exogenous genes in the sea urchin embryo
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Livant DL, Hough-Evans BR, Moore JG, Britten RJ, Davidson EH Differential stability of expression of similarly specified endogenous and exogenous genes in the sea urchin embryo. Development. 113:1991;385-398.
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(1991)
Development
, vol.113
, pp. 385-398
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Livant, D.L.1
Hough-Evans, B.R.2
Moore, J.G.3
Britten, R.J.4
Davidson, E.H.5
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10
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0026598853
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Modulation of sea urchin actin mRNA prevalence during embryogenesis: Nuclear synthesis and decay rate measurements of transcripts from five different genes
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Lee JJ, Calzone FJ, Davidson EH Modulation of sea urchin actin mRNA prevalence during embryogenesis: nuclear synthesis and decay rate measurements of transcripts from five different genes. Dev Biol. 149:1992;415-431.
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(1992)
Dev Biol
, vol.149
, pp. 415-431
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Lee, J.J.1
Calzone, F.J.2
Davidson, E.H.3
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11
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0030049992
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Spatial and temporal information processing in the sea urchin embryo: Modular and intramodular organization of the CyIIIa gene cis-regulatory system
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Kirchhamer CV, Davidson EH Spatial and temporal information processing in the sea urchin embryo: modular and intramodular organization of the CyIIIa gene cis-regulatory system. Development. 122:1996;333-348.
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(1996)
Development
, vol.122
, pp. 333-348
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Kirchhamer, C.V.1
Davidson, E.H.2
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12
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20644434934
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SpRunt-1, a new member of the Runt-domain family of transcription factors, is a positive regulator of the aboral ectoderm-specific CyIIIa gene in sea urchin embryos
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Coffman JA, Kirchhamer CV, Harrington MG, Davidson EH SpRunt-1, a new member of the Runt-domain family of transcription factors, is a positive regulator of the aboral ectoderm-specific CyIIIa gene in sea urchin embryos. Dev Biol. 174:1996;43-54.
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(1996)
Dev Biol
, vol.174
, pp. 43-54
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Coffman, J.A.1
Kirchhamer, C.V.2
Harrington, M.G.3
Davidson, E.H.4
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13
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0031468319
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SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos
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Coffman JA, Kirchhamer CV, Harrington MG, Davidson EH SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos. Development. 124:1997;4717-4727.
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(1997)
Development
, vol.124
, pp. 4717-4727
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Coffman, J.A.1
Kirchhamer, C.V.2
Harrington, M.G.3
Davidson, E.H.4
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14
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0032424433
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TKO, a general approach to functionally knock-out transcription factors in vivo, using a novel genetic expression vector
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A new method is described for controlling transcription factor activity in vivo, by a 'magic bullet' approach. A genetic expression construct encoding a single chain antibody against the P3A2 transcriptional repressor was injected into eggs, and shown to result in spatial derepression of both exogenous CyIIIa expression constructs and the endogenous CyIIa gene.
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Bogarad LD, Arnone MI, Chieh C, Davidson EH TKO, a general approach to functionally knock-out transcription factors in vivo, using a novel genetic expression vector. Proc Natl Acad Sci USA. 95:1998;14827-14832. A new method is described for controlling transcription factor activity in vivo, by a 'magic bullet' approach. A genetic expression construct encoding a single chain antibody against the P3A2 transcriptional repressor was injected into eggs, and shown to result in spatial derepression of both exogenous CyIIIa expression constructs and the endogenous CyIIa gene.
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(1998)
Proc Natl Acad Sci USA
, vol.95
, pp. 14827-14832
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Bogarad, L.D.1
Arnone, M.I.2
Chieh, C.3
Davidson, E.H.4
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15
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0029817680
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Modular cis-regulatory organization of developmentally expressed genes: Two genes transcribed territorially in the sea urchin embryo, and additional examples
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Kirchhamer CV, Yuh C-H, Davidson EH Modular cis-regulatory organization of developmentally expressed genes: two genes transcribed territorially in the sea urchin embryo, and additional examples. Proc Natl Acad Sci USA. 93:1996;9322-9328.
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(1996)
Proc Natl Acad Sci USA
, vol.93
, pp. 9322-9328
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Kirchhamer, C.V.1
Yuh, C.-H.2
Davidson, E.H.3
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16
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0030986188
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The hardwiring of development: Organization and function of genomic regulatory systems
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Arnone M, Davidson EH The hardwiring of development: organization and function of genomic regulatory systems. Development. 124:1997;1851-1864.
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(1997)
Development
, vol.124
, pp. 1851-1864
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Arnone, M.1
Davidson, E.H.2
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17
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0030013231
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Regulation of two pair-rule stripes by a single enhancer in the Drosophila embryo
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Small S, Blair A, Levine M Regulation of two pair-rule stripes by a single enhancer in the Drosophila embryo. Dev Biol. 175:1996;314-324.
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(1996)
Dev Biol
, vol.175
, pp. 314-324
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Small, S.1
Blair, A.2
Levine, M.3
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18
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0029863315
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Modular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo
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Yuh C-H, Davidson EH Modular cis-regulatory organization of Endo16, a gut-specific gene of the sea urchin embryo. Development. 122:1996;1069-1082.
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(1996)
Development
, vol.122
, pp. 1069-1082
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Yuh, C.-H.1
Davidson, E.H.2
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19
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0028968936
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A multimerizing transcription factor of sea urchin embryos capable of looping DNA
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Zeller RW, Griffith JD, Moore JG, Kirchhamer CV, Britten RJ, Davidson EH A multimerizing transcription factor of sea urchin embryos capable of looping DNA. Proc Natl Acad Sci USA. 92:1995;2989-2993.
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(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 2989-2993
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Zeller, R.W.1
Griffith, J.D.2
Moore, J.G.3
Kirchhamer, C.V.4
Britten, R.J.5
Davidson, E.H.6
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20
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0032549745
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Genomic cis-regulatory logic: Functional analysis and computational model of a sea urchin gene control system
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The first computational model of a developmental animal cis-regulatory system, viz. the Endo16 gene of S. purpuratus is presented in this paper. Detailed experimental analyses of the functional significance of transcription factor target sites in the proximal region of this system leads to its representation as an analog information processing device built into the DNA sequence.
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Yuh C-H, Bolouri H, Davidson EH Genomic cis-regulatory logic: functional analysis and computational model of a sea urchin gene control system. Science. 279:1998;1896-1902. The first computational model of a developmental animal cis-regulatory system, viz. the Endo16 gene of S. purpuratus is presented in this paper. Detailed experimental analyses of the functional significance of transcription factor target sites in the proximal region of this system leads to its representation as an analog information processing device built into the DNA sequence.
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(1998)
Science
, vol.279
, pp. 1896-1902
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Yuh, C.-H.1
Bolouri, H.2
Davidson, E.H.3
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21
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0025255418
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How embryos work: A comparative view of diverse modes of cell fate specification
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Davidson EH How embryos work: a comparative view of diverse modes of cell fate specification. Development. 108:1990;365-389.
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(1990)
Development
, vol.108
, pp. 365-389
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Davidson, E.H.1
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22
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0025854308
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Gene regulatory factors of the sea urchin embryo. I. Purification by affinity chromatography and cloning of P3A2, a novel DNA binding protein
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Calzone FJ, Höög C, Teplow DB, Cutting AE, Zeller RW, Britten RJ, Davidson EH Gene regulatory factors of the sea urchin embryo. I. Purification by affinity chromatography and cloning of P3A2, a novel DNA binding protein. Development. 112:1991;335-350.
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(1991)
Development
, vol.112
, pp. 335-350
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Calzone, F.J.1
Höög, C.2
Teplow, D.B.3
Cutting, A.E.4
Zeller, R.W.5
Britten, R.J.6
Davidson, E.H.7
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23
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0027135555
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NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of development regulators
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Virbasius CMA, Virbasius JV, Scarpulla RC NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of development regulators. Genes Dev. 7:1993;2431-2445.
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(1993)
Genes Dev
, vol.7
, pp. 2431-2445
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Virbasius, C.M.A.1
Virbasius, J.V.2
Scarpulla, R.C.3
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24
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0027240848
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The Drosophila erect wing gene, which is important for both neuronal and muscle development encodes a protein which is similar to the sea urchin P3A2 DNA-binding protein
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DeSimone SM, White K The Drosophila erect wing gene, which is important for both neuronal and muscle development encodes a protein which is similar to the sea urchin P3A2 DNA-binding protein. Mol Cell Biol. 13:1993;3641-3649.
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(1993)
Mol Cell Biol
, vol.13
, pp. 3641-3649
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Desimone, S.M.1
White, K.2
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25
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0030723860
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Green fluorescent protein in the sea urchin: New experimental approaches to transcriptional regulatory analysis in embryos and larvae
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Arnone MI, Bogarad LD, Collazo A, Kirchhamer CV, Cameron RA, Rast JP, Gregorians A, Davidson EH Green fluorescent protein in the sea urchin: new experimental approaches to transcriptional regulatory analysis in embryos and larvae. Development. 124:1997;4649-4659.
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(1997)
Development
, vol.124
, pp. 4649-4659
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Arnone, M.I.1
Bogarad, L.D.2
Collazo, A.3
Kirchhamer, C.V.4
Cameron, R.A.5
Rast, J.P.6
Gregorians, A.7
Davidson, E.H.8
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26
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0028929416
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SpZ12-1, a negative regulator required for spatial control of the territory-specific CyIIIa gene in the sea urchin embryo
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Wang DG-W, Kirchhamer CV, Britten RJ, Davidson EH SpZ12-1, a negative regulator required for spatial control of the territory-specific CyIIIa gene in the sea urchin embryo. Development. 121:1995;1111-1122.
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(1995)
Development
, vol.121
, pp. 1111-1122
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Wang, D.-W.1
Kirchhamer, C.V.2
Britten, R.J.3
Davidson, E.H.4
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27
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0029895168
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Transcriptional repression in development
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Gray S, Levine M Transcriptional repression in development. Curr Opin Cell Biol. 8:1996;358-364.
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Curr Opin Cell Biol
, vol.8
, pp. 358-364
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Gray, S.1
Levine, M.2
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28
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0024454393
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Endo16, a lineage-specific protein of the sea urchin embryo, is first expressed just prior to gastrulation
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Nocente-McGrath C, Brenner CA, Ernst SG Endo16, a lineage-specific protein of the sea urchin embryo, is first expressed just prior to gastrulation. Dev Biol. 136:1989;264-272.
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Dev Biol
, vol.136
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Nocente-Mcgrath, C.1
Brenner, C.A.2
Ernst, S.G.3
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29
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0027437450
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Whole mount in situ hybridization shows Endo-16 to be a marker for the vegetal plate territory in sea urchin embryos
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Ransick A, Ernst S, Britten RJ, Davidson EH Whole mount in situ hybridization shows Endo-16 to be a marker for the vegetal plate territory in sea urchin embryos. Mech Dev. 42:1993;117-124.
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(1993)
Mech Dev
, vol.42
, pp. 117-124
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Ransick, A.1
Ernst, S.2
Britten, R.J.3
Davidson, E.H.4
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30
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0030579165
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Alternative splicing of the Endo16 transcript produces differentially expressed mRNAs during sea urchin gastrulation
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Godin RE, Urry LA, Ernst SG Alternative splicing of the Endo16 transcript produces differentially expressed mRNAs during sea urchin gastrulation. Dev Biol. 179:1996;148-159.
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(1996)
Dev Biol
, vol.179
, pp. 148-159
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Godin, R.E.1
Urry, L.A.2
Ernst, S.G.3
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0032008552
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1 lineages to endodermal fate in the sea urchin embryo
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1 lineages to endodermal fate in the sea urchin embryo. Dev Biol. 195:1998;38-48.
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Dev Biol
, vol.195
, pp. 38-48
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Ransick, A.1
Davidson, E.H.2
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0030463511
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Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene
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Yuh C-H, Moore JG, Davidson EH Quantitative functional interrelations within the cis-regulatory system of the S. purpuratus Endo16 gene. Development. 122:1996;4045-4056.
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(1996)
Development
, vol.122
, pp. 4045-4056
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Yuh, C.-H.1
Moore, J.G.2
Davidson, E.H.3
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33
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0028065388
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Multiple Otx-binding sites required for expression of the Strongylocentrotus purpuratus Spec2A gene
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Mao CM, Gan L, Klein WH Multiple Otx-binding sites required for expression of the Strongylocentrotus purpuratus Spec2A gene. Dev Biol. 165:1994;229-242.
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Dev Biol
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, pp. 229-242
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Mao, C.M.1
Gan, L.2
Klein, W.H.3
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34
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0030789794
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Two Otx proteins generated from multiple transcripts of a single gene in Strongylocentrotus purpuratus
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Li X, Chuang CK, Mao CA, Angerer LM, Klein WH Two Otx proteins generated from multiple transcripts of a single gene in Strongylocentrotus purpuratus. Dev Biol. 187:1997;253-266.
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Dev Biol
, vol.187
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Li, X.1
Chuang, C.K.2
Mao, C.A.3
Angerer, L.M.4
Klein, W.H.5
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35
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0024566578
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Lineage-specific gene expression and the regulative capacities of the sea urchin embryo: A proposed mechanism
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Davidson EH Lineage-specific gene expression and the regulative capacities of the sea urchin embryo: a proposed mechanism. Development. 105:1989;421-445.
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(1989)
Development
, vol.105
, pp. 421-445
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Davidson, E.H.1
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0026603920
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Early mRNAs, spatially restricted along the animal-vegetal axis of sea urchin embryos, include one encoding a protein related to tolloid and BMP-1
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Reynolds SD, Angerer LM, Palis J, Nasir A, Angerer RC Early mRNAs, spatially restricted along the animal-vegetal axis of sea urchin embryos, include one encoding a protein related to tolloid and BMP-1. Development. 114:1992;769-786.
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Development
, vol.114
, pp. 769-786
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Reynolds, S.D.1
Angerer, L.M.2
Palis, J.3
Nasir, A.4
Angerer, R.C.5
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37
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0026597940
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Spatial expression of the hatching enzyme gene in the sea urchin embryo
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Lepage T, Sardet C, Gache C Spatial expression of the hatching enzyme gene in the sea urchin embryo. Dev Biol. 150:1992;23-32.
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(1992)
Dev Biol
, vol.150
, pp. 23-32
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Lepage, T.1
Sardet, C.2
Gache, C.3
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38
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0026536983
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Spatial and temporal expression pattern during sea urchin embryogenesis of a gene coding for a protease homologous to the human protein BMP-1 and to the product of the Drosophila dorsal-ventral patterning gene tolloid
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Lepage T, Ghiglione C, Gache C Spatial and temporal expression pattern during sea urchin embryogenesis of a gene coding for a protease homologous to the human protein BMP-1 and to the product of the Drosophila dorsal-ventral patterning gene tolloid. Development. 114:1992;147-164.
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Development
, vol.114
, pp. 147-164
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Lepage, T.1
Ghiglione, C.2
Gache, C.3
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39
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0028884801
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Characterization of the SpHE promoter that is spatially regulated along the animal-vegetal axis of the sea urchin embryo
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Wei Z, Angerer LM, Gagnon ML, Angerer RC Characterization of the SpHE promoter that is spatially regulated along the animal-vegetal axis of the sea urchin embryo. Dev Biol. 171:1995;195-211.
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Dev Biol
, vol.171
, pp. 195-211
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Wei, Z.1
Angerer, L.M.2
Gagnon, M.L.3
Angerer, R.C.4
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40
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0032906634
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Identification of a new sea urchin Ets protein, SpEts4, by yeast one-hybrid screening with the hatching enzyme promoter
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Wei Z, Angerer RC, Angerer LM Identification of a new sea urchin Ets protein, SpEts4, by yeast one-hybrid screening with the hatching enzyme promoter. Mol Cell Biol. 19:1999;1271-1278.
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Mol Cell Biol
, vol.19
, pp. 1271-1278
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Wei, Z.1
Angerer, R.C.2
Angerer, L.M.3
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41
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0031464538
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Organization of the proximal promoter of the hatching-enzyme gene, the earliest zygotic gene expressed in the sea urchin embryo
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Ghiglione C, Emily-Fenquil F, Lhomond G, Gache C Organization of the proximal promoter of the hatching-enzyme gene, the earliest zygotic gene expressed in the sea urchin embryo. Eur J Biochem. 250:1997;502-513.
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Eur J Biochem
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, pp. 502-513
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Ghiglione, C.1
Emily-Fenquil, F.2
Lhomond, G.3
Gache, C.4
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42
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0029938621
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Characterization of a SpAN promoter sufficient to mediate correct spatial regulation along the animal-vegetal axis of the sea urchin embryo
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Kozlowski DJ, Gagnon ML, Marchant JK, Reynolds SD, Angerer LM, Angerer RC Characterization of a SpAN promoter sufficient to mediate correct spatial regulation along the animal-vegetal axis of the sea urchin embryo. Dev Biol. 176:1996;95-107.
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Dev Biol
, vol.176
, pp. 95-107
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Kozlowski, D.J.1
Gagnon, M.L.2
Marchant, J.K.3
Reynolds, S.D.4
Angerer, L.M.5
Angerer, R.C.6
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43
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0024097539
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A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo
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Sucov HM, Hough-Evans BR, Franks RR, Britten RJ, Davidson EH A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo. Genes Dev. 2:1988;1238-1250.
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(1988)
Genes Dev
, vol.2
, pp. 1238-1250
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Sucov, H.M.1
Hough-Evans, B.R.2
Franks, R.R.3
Britten, R.J.4
Davidson, E.H.5
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44
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0029065789
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Cis-regulatory control of the SM50 gene, an early marker of skeletogenic lineage specification in the sea urchin embryo
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Makabe KW, Kirchhamer CV, Britten RJ, Davidson EH Cis-regulatory control of the SM50 gene, an early marker of skeletogenic lineage specification in the sea urchin embryo. Development. 121:1995;1957-1970.
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(1995)
Development
, vol.121
, pp. 1957-1970
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Makabe, K.W.1
Kirchhamer, C.V.2
Britten, R.J.3
Davidson, E.H.4
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45
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0032926482
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HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation in the sea urchin embryo
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Identification of an Ets class transcription factor as the sequence-specific regulator that binds in a region of the SM50 cis-regulatory system required for maximum levels of activity; the factor is localized to the skeletogenic cells.
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Kurokawa D, Kitajima T, Mitsunaga-Nakatsubo K, Ameniya S, Shimada H, Akasaka K HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation in the sea urchin embryo. Mech Dev. 80:1999;41-52. Identification of an Ets class transcription factor as the sequence-specific regulator that binds in a region of the SM50 cis-regulatory system required for maximum levels of activity; the factor is localized to the skeletogenic cells.
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Spatially regulated SpEts4 transcription factor activity along the sea urchin embryo animal-vegetal axis
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Message for SpEts4, a major positive regulator of the hatching enzyme gene of S. purpuratus (SpHE), is shown to be localized to the non-vegetal cells where the SzzpHE gene is transcriptionally expressed. Expression of an engrailed repressor domain fusion with SpEts4-prevents SpHE transcription and precludes hatching.
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Wei Z, Angerer LM, Angerer RC Spatially regulated SpEts4 transcription factor activity along the sea urchin embryo animal-vegetal axis. Development. 126:1998;1729-1737. Message for SpEts4, a major positive regulator of the hatching enzyme gene of S. purpuratus (SpHE), is shown to be localized to the non-vegetal cells where the SzzpHE gene is transcriptionally expressed. Expression of an engrailed repressor domain fusion with SpEts4-prevents SpHE transcription and precludes hatching.
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Asymmetric accumulation of Sox family transcription factor along the AV axis of sea urchin embryos
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in press. A Sox class transcription factor, SpSoxB1, is shown to be a major positive regulator of the SpAN metalloprotease gene. Confocal immunocytology shows that the factor is initially present in all nuclei, but is quickly lost from vegetal nuclei during cleavage, and zygotically produced factor is generated only in the non-vegetal nuclei where the SpAN gene is transcribed
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Kenny AP, Kozlowski D, Oleksyn DW, Angerer LM, Angerer RC: Asymmetric accumulation of Sox family transcription factor along the AV axis of sea urchin embryos. Development 1999, in press. A Sox class transcription factor, SpSoxB1, is shown to be a major positive regulator of the SpAN metalloprotease gene. Confocal immunocytology shows that the factor is initially present in all nuclei, but is quickly lost from vegetal nuclei during cleavage, and zygotically produced factor is generated only in the non-vegetal nuclei where the SpAN gene is transcribed.
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Development
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Kenny, A.P.1
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48
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49
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Nuclear β-catenin is required to specify vegetal cell fates in the sea urchin embryo
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Initial decisive experiments demonstrating the role of β-catenin nuclearization in endomesoderm specification are described in this paper. Overexpression and prevention of β-catenin nuclearization, respectively, cause excess and deficient specification of endomesoderm. β-catenin nuclearization exactly correlates with endomesodermal fate, and occurs autonomously.
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Logan CY, Miller JR, Ferkowicz MJ, McClay DR Nuclear β-catenin is required to specify vegetal cell fates in the sea urchin embryo. Development. 126:1999;345-357. Initial decisive experiments demonstrating the role of β-catenin nuclearization in endomesoderm specification are described in this paper. Overexpression and prevention of β-catenin nuclearization, respectively, cause excess and deficient specification of endomesoderm. β-catenin nuclearization exactly correlates with endomesodermal fate, and occurs autonomously.
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50
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β-Catenin is essential for patterning the maternally specified animal-vegetal axis in the sea urchin embryo
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Expression of mRNA encoding cadherin, which traps β-catenin in the cytoplasm, causes failure of endomesoderm specification; and expression of mRNA encoding β-catenin causes increased endomesoderm specification.
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in press. The sea urchin TCF cDNA is isolated and the gene is shown to be expressed early in development at exactly the time required for this molecule to participate in endomesoderm specification. Embryonic expression of mRNA encoding a negative form of TCF disrupts endomesoderm (as well as aboral ectoderm) specification
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Huang L, Wikramanayake AH, Li X, el-Hodiri HM, Klein WH: Early transient expression of Strongylotcentrotus purpuratus Tcf/Lef and involvement in establishing cell types along he animal-vegetal axis. Dev Genes Evol 1999, in press. The sea urchin TCF cDNA is isolated and the gene is shown to be expressed early in development at exactly the time required for this molecule to participate in endomesoderm specification. Embryonic expression of mRNA encoding a negative form of TCF disrupts endomesoderm (as well as aboral ectoderm) specification.
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Dev Genes Evol
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Huang, L.1
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Klein, W.H.5
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52
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TCF is a nuclear effector of a β-catenin signal in patterning the sea urchin AV axis
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in press. Expression in sea urchin eggs of mRNA encoding an inactive form of Xenopus TCF results in failure of vegetal specification
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Vonica A, Weng W, Gumbiner BM, Venuti JM: TCF is a nuclear effector of a β-catenin signal in patterning the sea urchin AV axis. Dev Biol 1999, in press. Expression in sea urchin eggs of mRNA encoding an inactive form of Xenopus TCF results in failure of vegetal specification.
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GSK3β/shaggy mediates patterning along the animal-vegetal axis of the sea urchin embryo
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Expression of mRNA encoding an inactive form of GSK3 caused increase in the vegetal domain of the embryo, and reduced the animal territory in which the hatching enzyme gene is transcribed. Overexpression of normal GSK3 had the converse effects, increasing the animal ectodermal territory at the expense of the endomesoderm.
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Decisive demonstration that Notch signaling is required for mesoderm specification: expression of mRNA encoding a negatively-acting Notch ligand abolishes mesoderm specification; and expression of an mRNA encoding a constitutively active form of Notch produces excess mesoderm specification.
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Expression of mRNA encoding the SpMsx transcription factor affects ectoderm cells in such a way as to disrupt the intercellular interactions required for skeletogenic mesenchyme cells to form the correct pattern on the blastocoel wall.
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