Voltage-gated Na+ channel activity and connexin expression in Cx43- deficient cardiac myocytes

Journal of Cardiovascular Electrophysiology

Volumn 10, Issue 10, 1999, Pages 1390-1401

  Johnson, Carolyn M ^a   Green, Karen G ^a   Kanter, Evelyn M ^a   Bou Abboud, Elias ^a   Saffitz, Jeffrey E ^a   Yamada, Kathryn A ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Action potentials; Confocal microscopy; Connexin43; Connexin43 null; Immunofluorescence; Mice; Sodium current; Ventricular myocytes; Voltage gated sodium channels

Indexed keywords

GAP JUNCTION PROTEIN; SODIUM CHANNEL;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HEART CONDUCTION; HEART CONTRACTION; HEART DEPOLARIZATION; HEART MUSCLE CELL; HEART MUSCLE FIBER MEMBRANE; HEART MUSCLE POTENTIAL; HEART REPOLARIZATION; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SODIUM CURRENT;

EID: 0032870163     PISSN: 10453873     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1540-8167.1999.tb00195.x     Document Type: Article

References (27)

1. Kanter HL, Saffitz JE, Beyer EC: Cardiac myocytes express multiple gap junction proteins. Circ Res 1992; 70:438-444.
2. Oosthoek PW, Viragh S, Mayen AEM, et al: Immuno-histochemical delineation of the conduction system. I. The sinoatrial node. Circ Res 1993;78:473-481.
3. Oosthoek PW, Viragh S, Lamers WH, et al: Immuno-histochemical delineation of the conduction system. II. The atrioventricular node and the Purkinje fibers. Circ Res 1993;78:482-491.
4. Gourdie RG, Severs NH, Green CR, et al: The spatial distribution and relative abundance of gap-junctional connexin40 und connexin43 correlate to functional properties of components of the cardiac atrioventricular conduction system. J Cell Sci 1993; 105:985-991.
5. Bastide B, Neyses L, Ganten D, et al: Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ Res 1993;73:1138-1149.
6. Davis LM, Kanter HL, Beyer EC, et al: Distinct gap junction protein phenotypes in cardiac tissues with disparate conduction properties. J Am Coll Cardiol 1994; 24:1124-1132.
7. Saffitz JE, Kanter HL, Green KG, et al: Tissue-specific determinants of anisotropic conduction velocity in canine atrial and ventricular myocardium, Circ Res 1994; 74:1065-1070.
8. Gros D, Jarry-Guichard T, Ten Velde T, et al: Restricted distribution of connexin40, a gap junctional protein, in mammalian heart. Circ Res 1994;74:839-851.
9. Coppen SR, Dupont E, Rothery S, et al: Connexin45 expression is preferentially associated with the ventricular conduction system in the mouse. Circ Res 1998; 82:232-243.
10. Kanter HL, Beyer EC, Green KG et al: Multiple connexins colocalize in canine cardiac myocyte gap junctions. Circ Res 1993;74:344-350.
11. Reaume AG, de Sousa PA, Kulkarni S, et al: Cardiac malformation in neonatal mice lacking connexin43. Science 1995;267:1831-1834.
12. Ya J, Erdtsieck-Ernste EBHW, de Boer PAJ, et al: Heart defects in connexin43-deficient mice. Circ Res 1998;82:360-366.
13. Guerrero PA, Schuessler RB, Davis LM, et al: Slow ventricular conduction in mice heterozygous for a connexin43 null mutation. J Clin Invest 1997;99:1991-1998.
14. Thomas SA, Schuessler RB, Berul CI, et al: Disparate effects of deficient expression of connexin43 on atrial and ventricular conduction. Evidence for chamber-specific molecular determinants of conduction. Circulation 1998;97:686-691.
15. Johnson CM, Kanter EM, Green KG, et al: Ventricular connexin expression and gap junction structure in Cx43 knockout mice. (Abstract) Circulation 1998;98:I-256.
16. Veenstra RD, Wang H-Z, Beyer EC, et al: Selective dye and ionic permeability of gap junction channels formed by connexin45. Circ Res 1994;75:483-490.
17. Hamill OP, Marty A, Neher E, et al: Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 1981;391:85-100.
18. Saffitz JE, Green KG, Kraft WJ, et al: Effects of diminished expression of Cx43 on gap junction number and size in ventricular myocardium. (Abstract) Circulation 1998;98:I-256.
19. Nuss HB, Marban E: Electrophysiological properties of neonatal mouse cardiac myocytes in primary culture. J Physiol (Lond) 1994;479:265-279.
20. + channels in mouse heart. Circ Res 1996;79:79-85.
21. Wang L, Duff HJ: Developmental changes in transient outward current in mouse ventricle. Circ Res 1997;81: 120-127.
22. Johnson CM, Guerrero PA, Kanter EM, et al: Cellular electrophysiology of ventricular myocytes isolated from neonatal mice deficient in connexin43. (Abstract) PACE 1997;20:1050.
23. Cohen SA: Immunocytochemical localization of rH1 sodium channel in adult rat heart atria and ventricle. Presence in terminal intercalated disks. Circulation 1996;94:3083-3086.
24. + channel protein in explanted cardiac tissue. J Clin Invest 1995;96:282-292.
25. Peters NS, Severs NJ, Rothery S, et al: Spatiotemporal relations between gap junctions and fascia adherens junctions during postnatal development of human ventricular myocardium. Circulation 1994;90:713-725.
26. Shaw RM, Rudy Y: Ionic mechanisms of propagation in cardiac tissue. Roles of the sodium and L-type calcium currents during reduced excitability and decreased gap junction coupling. Circ Res 1997;81:727-741.
27. Spray DC, Vink MJ, Scemes E, et al: Characteristics of coupling in cardiac myocytes and CNS astrocytes cultured from wildtype and Cx43-null mice. In Werner R, ed: Gap Junctions. IOS Press, Amsterdam, 1998. pp. 281-285.