Standardisation and quality assurance of lymphocyte proliferation assays for use in the assessment of immune function

Journal of Immunological Methods

Volumn 227, Issue 1-2, 1999, Pages 85-97

  Froebel, K S ^a   Pakker, N G ^b   Aiuti, F ^c   Bofill, M ^d   Choremi Papadopoulou, H ^e   Economidou, J ^f   Rabian, C ^g   Roos, M T L ^b   Ryder, L P ^h   Miedema, F ^b   Raab, G M ⁱ  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b CENTRAL LABORATORY   (Netherlands)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

^d ROYAL FREE HOSPITAL   (United Kingdom)

^e LAIKO GENERAL HOSPITAL   (Greece)

^f EVANGELISMOS HOSPITAL   (Greece)

^g SAINT LOUIS HOSPITAL   (France)

^h Rigshospitalet   (Denmark)

ⁱ EDINBURGH NAPIER UNIVERSITY   (United Kingdom)

Author keywords

Antibody mediated stimulation; Assay; HIV disease progression; Lymphocyte proliferation; Mitogen stimulation

Indexed keywords

ANTIBODY; MITOGENIC AGENT;

ARTICLE; ASSAY; CALCULATION; CASE REPORT; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IMMUNE FUNCTION TEST; INFORMATION PROCESSING; LYMPHOCYTE PROLIFERATION; OBSERVER VARIATION; PRIORITY JOURNAL; QUALITY CONTROL; STANDARDIZATION; TIME;

EID: 0032868637     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00082-4     Document Type: Article

References (14)

1. Bloemena E., Roos M.T., Van Heijst J.L., Vossen J.M., Schellekens P.T. Whole-blood lymphocyte cultures. J. Immunol. Methods. 122:1989;161.
2. Bocchieri M.H., Talle M.A., Maltese L.M., Ragucci I.R., Hwang C.-C., Goldstein G. Whole blood culture for measuring mitogen induced T cell proliferation provides superior correlations with disease state and T cell phenotype in asymptomatic HIV-infected subjects. J. Immunol. Methods. 181:1995;233-243.
3. Ebert E.C., Roberts A.I. Costimulation of the CD3 pathway by CD28 ligation in human intestinal lymphocytes. Cell Immunol. 171:1996;211.
4. Hofmann B., Jakobsen K.D., Odum N., Dickmeiss E., Platz P., Ryder L.P., Pendersen C., Mathiesen L., Bygbjerg I., Faber V., Svejgaard A. HIV induced immunodeficiency. Relatively preserved phytohemagglutinin as opposed to decreased pokeweed mitogen responces may be due to possibly preserved responces via CD2/phytohemagglutinin pathway. J. Immunol. 142:1989;1874.
5. Jenkins M.K., Taylor P.S., Norton S.D., Urdahl K.B. CD28 delivers a costimulatory signal involved in antigen-specific IL-2 production by human T cells. J. Immunol. 147:1991;2461.
6. Kirkwood T. Geometric means and measures of dispersion. Biometrics. 35:1979;909-910.
7. Li T.S., Tubiana R., Katlama C., Calvez V., Ait Mohand H., Autran B. Long-lasting recovery in CD4 T-cell function and viral load reduction after highly active antiretroviral therapy in advanced HIV-1 disease. Lancet. 351:1998;1682-1686.
8. Meyaard L., Kuiper H., Otto S.A., Wolthers K.C., van Lier R.A., Miedema F. Evidence for intact costimulation via CD28 and CD27 molecules in hyporesponsive T cells from human immunodeficiency virus-infected individuals. Eur. J. Immunol. 25:1995;232.
9. Nisbet-Brown E.R., Lee J.W.W., Cheung R.K., Gelfand E.W. Antigen-specific and -nonspecific mitogenic signals in the activation of human T cell clones. J. Immunol. 138:1987;3713.
10. Pakker N.G., Notermans D.W., de Boer R.J., Roos M.T., De Wolf F., Hill A., Leonard J.M., Danner S.A., Miedema F., Schellekens P.T. Biphasic kinetics of peripheral blood T cells after triple combination therapy in HIV-1 infection: a composite of redistribution and proliferation. Nat. Med. 4:1998;208.
11. Roos M.T., Miedema F., Meinesz A.P., De Leeuw N.A., Pakker N.G., Lange J.M., Coutinho R.A., Schellekens P.T.A. Low T cell reactivity to combined CD3 plus CD28 stimulation is predictive for progression to AIDS: correlation with decreased CD28 expression. Clin. Exp. Immunol. 105:1996;409.
12. Schellekens P.T.A., Roos M.T.L., De Wolf F., Lange J.M.A., Miedema F. Low T-cell responsiveness to activation via CD3/TCR is a prognostic marker for acquired immunodeficiency syndrome (AIDS) in human immunodeficiency virus-1 (HIV-1) infected men. J. Clin Immunol. 10:1989;121.
13. van Lier R.A., Boot J.H., Verhoeven A.J., De Groot E.R., Brouwer M., Aarden L.A. Functional studies with anti-CD3 heavy chain isotype switch-variant monoclonal antibodies. Accessory cell-independent induction of interleukin 2 responsiveness in T cells by epsilon-anti-CD3. J. Immunol. 139:1987;2873.
14. van Lier R.A., Brouwer M., Aarden L.A. Signals involved in T cell activation. T cell proliferation induced through the synergistic action of anti-CD28 and anti-CD2 monoclonal antibodies. Eur. J. Immunol. 18:1988;167.