High-speed detection of the two common α1-antitrypsin deficiency alleles Pi*Z and Pi*S by real-time fluorescence PCR and melting curves

Clinical Chemistry

Volumn 45, Issue 10, 1999, Pages 1872-1875

  Aslanidis, Charalampos ^a   Nauck, Markus ^b   Schmitz, Gerd ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTITRYPSIN;

ALLELISM; ALPHA 1 ANTITRYPSIN DEFICIENCY; ARTICLE; FLUORESCENCE ANALYSIS; GENE MUTATION; GENETIC ANALYSIS; HUMAN; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS;

EID: 0032857973     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/45.10.1872     Document Type: Article

References (8)

1. 1. Crystal RG. The α-1-antitrypsin gene and its deficiency states. Trends Genet 1989;5:411-7.
2. 2. Crystal RG. α-1-Antitrypsin deficiency, emphysema, and liver disease. Genetic basis and strategies of therapy. J Clin Investig 1990;85:1343-52.
3. 1-Antitrypsin deficiency in Europe: geographical distribution of Pi types S and Z. Respir Med 1998;92:367-77.
4. 1 proteinase inhibitor deficiency alleles Pi*Z and Pi*S by DNA analysis. Eur J Clin Chem Clin Biochem 1996;34:761-4.
5. 5. Wittwer C, Ririe K, Andrew R, David D, Gundry R, Balis U. The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 1997;22:176-81.
6. 6. Lay M, Wittwer C. Real time fluorescence genotyping of factor V Leiden during rapid cycle PCR. Clin Chem 1997;43:2262-7.
7. 7. Aslanidis C, Schmitz G. High-speed apolipoprotein E genotyping and apolipoprotein B3500 mutation detection using real-time fluorescence PCR and melting curves. Clin Chem 1999;45:1094-7.
8. 8. Aslanidis C, Nauck M, Schmitz G. High-speed prothrombin G→A 20210 and methylenetetrahydrofolate reductase C→T 677 mutation detection using real-time fluorescence PCR and melting curves. Biotechniques 1999;27:234-8.