Slice blotting: A method for detecting the release of immunoreactive substances from living brain tissue

Journal of Neuroscience Methods

Volumn 90, Issue 2, 1999, Pages 117-127

  Lowe, Graeme ^a  

^a MONELL CHEMICAL SENSES CENTER   (United States)

Author keywords

Blot; Brain slice; Immunoblotting; Mapping; Neuropeptide Y; Secretion; Semaphorin

Indexed keywords

NEUROPEPTIDE Y; POTASSIUM; SEMAPHORIN 3A;

ANIMAL TISSUE; ARTICLE; BRAIN; BRAIN SLICE; CYTOKINE RELEASE; ENZYME RELEASE; FLUORESCENCE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; SIGNAL TRANSDUCTION; TECHNIQUE;

ANIMALS; BRAIN MAPPING; BRAIN STEM; GLYCOPROTEINS; IMMUNOBLOTTING; MALE; MESENCEPHALON; NEUROPEPTIDE Y; OLFACTORY BULB; RATS; RATS, SPRAGUE-DAWLEY; SEMAPHORIN-3A; TISSUE SURVIVAL;

EID: 0032855498     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00074-6     Document Type: Article

References (69)

1. Aghajanian G.K., Rasmussen K. Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices. Synapse. 3:1989;331-338.
2. Aitken P.G., Breese G.R., Dudek F.F. et al. Preparative methods for brain slices: a discussion. J. Neurosci. Methods. 59:1995;139-149.
3. Alger BE, Dhanjal SS, Dingledine R, Garthwaite J, Herderson G, King GL, Lipton P, North A, Schwartzkroin PA, Sears TA, Segal M, Whittingham TS, Williams J. Brain slice methods. In: Dingledine R, editor. Brain Slices. New York: Plenum Press, 1984:381.
4. Allen T.G.J. The 'sniffer-patch' technique for detection of neurotransmider release. Trends. Neurosci. 20:1997;192-197.
5. Arita J. Analysis of the secretion from single anterior pituitary cells by cell immunoblot assay. Endocr. J. 40:1993;1-15.
6. Arita J., Yasuhiro K., Kimura F. Identification by the sequential cell immunoblot assay of a subpopulation of rat dopamine-unresponsive lactotrophs. Endocrinology. 128:1991;1887-1894.
7. Auerbach S., Lipton P. Vasopressin augments depolarization-induced release and synthesis of serotonin in hippocampal slices. J. Neurosci. 2:1982;477-482.
8. Ayoub GS, Korenbrot JI, Copenhagen DR. Release of endogenous glutamate from isolated cone photoreceptors of the lizard. Neurosci Res 1989;Suppl 10:47-56.
9. Ayoub G.S., Copenhagen D.R. Application of a fluorometric method to measure glutamate release from single retinal photoreceptors. J. Neurosci. Methods. 37:1991;7-14.
10. Ayoub G.S., Grutsis S., Simko H. Imaging of endogenous neurotransmitter release. J. Neurosci. Methods. 81:1998;110-113.
11. Bach-y-Rita P. Nonsynaptic diffusion neurotransmission (NDN) in the brain. Neurochem. Int. 23:1993;297-318.
12. Barbour B., Hausser M. Intersynaptic diffusion of neurotransmitter. Trends. Neurosci. 20:1997;377-384.
13. Behar O., Golden J.A., Mashimo H., Schoen F.J., Fishman M.C. Semaphorin III is needed for normal patterning and growth of nerves, bones and heart. Nature. 383:1996;525-528.
14. Beigelman P.M., Thomas L.J., Slavin B., Shu M.J., Bessman S.P. Insulin from individual isolated islets of Langerhans. I. Response to glucose. Biochem. Med. 8:1973;392-402.
15. Beigi R., Kobatake E., Aizawa M., Dubyak G.R. Detection of local ATP release from activated platelets using cell surface-attached firefly luciferase. Am. J. Physiol. 276:1999;C267-C278.
16. Benke D., Wenzel A., Scheuer L., Fritschy J.M., Mohler H. Immunobiochemical characterization of the NMDA-receptor subunit NR1 in the developing and adult rat brain. J. Recept. Signal Transduct. Res. 15:1995;393-411.
17. Betz W.J., Bewick G.S. Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction. Science. 255:1992;200-203.
18. Bjelke B., England R., Nicholson C., Rice M.E., Lindberg J., Zoli M., Agnati L.F., Fuxe K. Long distance pathways of diffusion for dextran along fibre bundles in brain. Relevance for volume transmission. Neuroreport. 6:1995;1005-1009.
19. Budrene E.O., Berg H.C. Dynamics of formation of symmetrical patterns by chemotactic bacteria. Nature. 376:1995;49-53.
20. Chen H.-L., Romsos D.R. Dexamethasone rapidly increases hypothalamic neuropeptide Y secretion in adrenalectomized ob/ob mice. Am. J. Physiol. 271:1996;E151-E158.
21. Ciarleglio A.K., Beinfeld M.C., Westfall T.C. Pharmacological characterization of the release of neuropeptide Y-like immunoreactivity from the rat hypothalamus. Neuropharmacology. 32:1993;819-825.
22. Colmers WF, Wahlstedt C, editors. The Biology of Neuropeptide Y and Related Peptides. Totowa, NJ: Humana Press, 1993.
23. Copenhagen D.R., Jahr C.E. Release of endogenous excitatory amino acids from turtle photoreceptors. Nature. 341:1989;536-539.
24. Culotti J.G., Kolodkin A.L. Functions of netrins and semaphorins in axon guidance. Curr. Opin. Neurobiol. 6:1996;81-88.
25. Daughaday W.H., Deuel T.F. Tumor secretion of growth factors. Endocrinol. Metab. Clin. North Am. 20:1991;539-563.
26. De Quidt M.E., Emson P.C. Distribution of neuropeptide Y-like immunoreactivity in the rat central nervous system-II. Immunohistochemical analysis. Neuroscience. 18:1986;545-618.
27. Duggan A.W., Hendry I.A. Laminar localization of sites of release of immunoreactive substance P in the dorsal horn with antibody coated microelectrodes. Neurosci. Lett. 68:1986;134-140.
28. Duggan A.W., Hendry I.A., Green J.L., Morton C.R., Hutchison W.D. The preparation and use of antibody microprobes. J. Neurosci. Methods. 23:1988;241-247.
29. Duggan A.W. Detection of neuropeptide release in the central nervous system with antibody microprobes. J. Neurosci. Methods. 34:1990;47-52.
30. Fuxe K, Agnati LF, editors. Volume Transmission in the Brain: Novel Mechanisms for Neural Transmission. New York: Raven Press, 1991.
31. Giger R.J., Pasterkamp R.J., Heijnen S., Holtmaat A.J., Verhaagen J. Anatomical distribution of the chemorepellent semaphorin III/collapsin-1 in the adult rat and human brain: predominant expression in structures of the olfactory-hippocampal pathway and the motor system. J. Neurosci. Res. 52:1998;27-42.
32. Gilbert SF. Developmental biology, fifth ed. Sinauer Associates: Sunderland, Massachusetts, 1997.
33. Harlow E, Lane D. Antibodies. A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 1988.
34. Hayashi S., Aihara M., Wakabayashi K. Content and distribution pattern of luteinizing hormonereleasing hormone (LHRH) in the hypothalamus of neonatally estrogenized female rats. Neurosci. Res. 12:1991;366-378.
35. Hume RI, Role LW, Fischbach GD. Acetylcholine release from growth cones detected with patches of acetylcholine receptor-rich membranes. Nature 1983:632-7.
36. Imayama S., Shimozono Y., Urabe A., Hori Y. A simple method for measuring the amount of immunoglobulin A secreted onto the skin surface. Acta Derm. Venereol. 75:1995;212-217.
37. 2 tension in adult and neonatal brain slices under several experimental conditions. Brain. Res. 568:1991;159-164.
38. Justice JB. Voltammetry in the neurosciences. Clifton, NJ: Humana Press, 1987.
39. Kalra S.P., Dube M.G., Pu S., Xu B., Horvath T.L., Kalra P.S. Interacting appetite-regulating pathways in the hypothalamic regulation of body weight. Endocr. Rev. 20:1999;68-100.
40. Kelly M.J., Condon T.P., Levine J.E., Ronnekliev O.K. Combined electrophysiological, immunocytochemical and peptide release measurements in the hypothalamic slice. Brain Res. 345:1985;264-270.
41. Kendall M.E., Hymer W.C. Cell blotting: a new approach to quantify hormone secretion from individual rat pituitary cells. Endocrinology. 121:1987;2260-2262.
42. Kendall M.E., Hymer W.C. Measurement of hormone secretion from individual cells by cell blot assay. Methods Enzymol. 168:1989;327-338.
43. Kingsnorth A.N., Slavin J. Peptide growth factors and wound healing. Br. J. Surg. 78:1991;1286-1290.
44. Kobayashi H., Koppel A.M., Luo Y., Raper J.A. A role for collapsin-1 in olfactory and cranial sensory axon guidance. J. Neurosci. 17:1997;8339-8352.
45. Köhler C., Eriksson L., Davies S., Chan-Palay V. Neuropeptide Y innervation of the hippocampal region in the rat and monkey brain. J. Comp. Neurol. 244:1986;384-400.
46. Krajewski S., Zapata J.M., Reed J.C. Detection of multiple antigens on western blots. Anal. Biochem. 236:1996;221-228.
47. Luo Y., Raible D., Raper J.A. Collapsin: a protein in brain that induces the collapse and paralysis of neuronal growth cones. Cell. 75:1993;217-227.
48. Marti E., Takada R., Bumcrot D.A., Sasaki H., McMahon A.P. Distribution of Sonic hedgehog peptides in the developing chick and mouse embryo. Development. 121:1995;2537-2547.
49. Martin C.S., Bronstein I. Imaging of chemiluminescent signals with cooled CCD camera systems. J. Biolumin. Chemilumin. 9:1994;145-153.
50. Mehler M.F., Mabie P.C., Zhang D., Kessler J.A. Bone morphogenetic proteins in the nervous system. Trends Neurosci. 20:1997;309-317.
51. Melkonyan H.S., Chang W.C., Shapiro J.P., Mahadevappa M., Fitzpatrick P.A., Kiefer M.C., Tomei L.D., Umansky S.R. SARPs: a family of secreted apoptosis-related proteins. Proc. Natl. Acad. Sci. USA. 94:1997;13636-13641.
52. Messersmith E.K., Leonardo E.D., Shatz C.J., Tessier-Lavigne M., Goodman C.S., Kolodkin A.L. Semaphorin III can function as a selective chemorepellent to pattern sensory projections in the spinal cord. Neuron. 14:1995;949-959.
53. Moyer J.R., Brown T.H. Methods for whole-cell recording from visually preselected neurons of perirhinal cortex in brain slices from young and aging rats. J. Neurosci. Methods. 86:1998;3554.
54. Myers R.D., Adell A., Lankford M.F. Simultaneous comparison of cerebral dialysis and push-pull perfusion in the brain of rats: a critical review. Neurosci. Biobehav. Rev. 22:1998;371-387.
55. Neher E., Marty A. Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells. Proc. Natl. Acad. Sci. USA. 79:1982;6712-6716.
56. Okabe M., Nyakas C., Buwalda B., Luiten P.G. In situ blotting: a novel method for direct transfer of native proteins from sectioned tissue to blotting membrane: procedure and some applications. J. Histochem. Cytochem. 41:1993;927-934.
57. Pasterkamp R.J., De Winter F., Holtmaat A.J., Verhaagen J. Evidence for a role of the chemorepellent semaphorin III and its receptor neuropilin-1 in the regeneration of primary olfactory axons. J. Neurosci. 18:1998;9962-9976.
58. Robinson TE, Justice JB, editors. Microdialysis in the Neurosciences. Techniques in the Behavioral and Neural Sciences, vol 7. Amsterdam: Elsevier, 1992.
59. Sabatini B.L., Regehr W.G. Optical measurement of presynaptic calcium currents. Biophys. J. 74:1998;1549-1563.
60. Stanley E.F. Single calcium channels and acetylcholine release at a presynaptic nerve terminal. Neuron. 11:1993;1007-1011.
61. Sternberger L.A., Sternberger N.H. The unlabeled antibody method: comparison of peroxidase-antiperoxidase with avidin-biotin complex by a new method of quantification. J. Histochem. Cytochem. 34:1986;599-605.
62. Taraboulos A., Jendroska K., Serban D., Yang S.L., DeArmond S.J., Prusiner S.B. Regional mapping of prion proteins in brain. Proc. Natl. Acad. Sci. USA. 89:1992;7620-7624.
63. Teruya K., Shirahata S., Yano T., Seki K., Tachibana H., Ohashi H., Murakami H. Secretory cell immunoscreening assay - a highly sensitive screening method for secretory cells. Anal. Biochem. 214:1993;468-473.
64. Tessier-Lavigne M., Goodman C.S. The molecular biology of axon guidance. Science. 274:1996;1123-1133.
65. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
66. Wightman R.M., Jankowski J.A., Kennedy R.T. et al. Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells. Proc. Natl. Acad. Sci. USA. 88:1991;10754-10758.
67. Yamamoto C., McIlwain H. Electrical activities in thin sections from the mammalian brain maintained in chemically-defined media in vitro. J. Neurochem. 13:1966;1333-1343.
68. Yamazoe M., Shiosaka S., Emson P.C., Tohyama M. Distribution of neuropeptide Y in the lower brainstem: an immunohistochemical analysis. Brain Res. 335:1985;109-120.
69. Young S.H., Poo M.-M. Spontaneous release of transmitter from growth cones of embryonic neurones. Nature. 305:1983;634-637.