The rat cytomegalovirus R32 gene encodes a virion-associated protein that elicits a strong humoral immune response in infected rats

Journal of General Virology

Volumn 80, Issue 10, 1999, Pages 2719-2728

  Beuken, Erik ^a   Grauls, Gert ^a   Bruggeman, Cathrien A ^a   Vink, Cornells ^a  

^a MAASTRICHT UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; EMBRYO; FIBROBLAST; GENETIC TRANSCRIPTION; HUMORAL IMMUNITY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; RAT; VIRION; VIRUS GENE; VIRUS IMMUNITY;

EID: 0032852534     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-80-10-2719     Document Type: Article

References (36)

1. Beisser, P. S., Vink, C., Van Dam, J. G., Grauls, G., Vanherle, S. J. V. & Bruggeman, C. A. (1998a). The R33 G protein-coupled receptor gene of rat cytomegalovirus plays an essential role in the pathogenesis of viral infection. Journal of Virology 72, 2352-2363.
2. Beisser, P. S., Kaptein, S. J. F., Beuken, E., Bruggeman, C. A. & Vink, C. (1998b). The Maastricht strain and England strain of rat cytomegalovirus represent different betaherpesvirus species rather than strains. Virology 246, 341-351.
3. Benko, D. M., Haltiwanger, R. S., Hart, G. W. & Gibson, W. (1988). Virion basic phosphoprotein from human cytomegalovirus contains O-linked N-acetylglucosamine. Proceedings of the National Academy of Sciences, USA 85, 2573-2577.
4. Beuken, E., Slobbe, R., Bruggeman, C. A. & Vink, C. (1996). Cloning and sequence analysis of the genes encoding DNA polymerase, glycoprotein B, ICP18.5 and major DNA-binding protein of rat cytomegalovirus. Journal of General Virology 77, 1559-1562.
5. Brown, T. & Mackey, K. (1997). Analysis of RNA by Northern and slot blot hybridization. In Current Protocols in Molecular Biology, pp. 4.9.1-4.9.6. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: John Wiley.
6. Bruggeman, C. A., Meijer, H., Bosman, F. & van Boven, C. P. A. (1985). Biology of rat cytomegalovirus infection. Intervirology 24, 1-9.
7. Bruning, J. H., Debie, W. H. M., Dormans, P. H. J., Meijer, H. & Bruggeman, C. A. (1987). The development and characterization of monoclonal antibodies against rat cytomegalovirus induced agents. Archives of Virology 94, 55-70.
8. Chee, M. S., Bankier, A. T., Beck, S., Bohni, R., Brown, C. M., Cerny, R., Horsnell, T., Hutchison, C. A., III, Kouzarides, T., Martignetti, J. A., Preddie, E., Satchwell, S. C., Tomlinson, P., Weston, K. M. & Barrell, B. G. (1990). Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154, 125-169.
9. Cranmer, L. D., Clark, C. & Spector, D. H. (1994). Cloning, characterization, and expression of the murine cytomegalovirus homologue of the human cytomegalovirus 28-kDa matrix phosphoprotein (UL99). Virology 205, 417-429.
10. Cranmer, L. D., Clark, C. L., Morello, C. S., Farrell, H. E., Rawlinson, W. D. & Spector, D. H. (1996). Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phospho-proteins. Journal of Virology 70, 7929-7939.
11. Gallina, A., Percivalle, E., Simoncini, L., Revello, M. G., Gerna, G. & Milanesi, G. (1996). Human cytomegalovirus pp65 lower matrix phosphoprotein harbours two transplantable nuclear localization signals. Journal of General Virology 77, 1151-1157.
12. Gibson, W. (1983). Protein counterparts of human and simian cytomegaloviruses. Virology 128, 391-406.
13. Gompels, U. A., Nicholas, J., Lawrence, G., Jones, M., Thomson, B. J., Martin, M. E. D., Efstathiou, S., Craxton, M. & Macaulay, H. A. (1995). The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution. Virology 209, 29-51.
14. Harlow, E. & Lane, D. (1988). Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
15. Hensel, G., Meyer, H., Gärtner, S., Brand, G. & Kern, H. F. (1995). Nuclear localization of the human cytomegalovirus tegument protein pp150 (ppUL32). Journal of General Virology 76, 1591-1601.
16. Hofmann, K., Bucher, P., Falquet, L. & Bairoch, A. (1999). The PROSITE database, its status in 1999. Nucleic Acids Research 27, 215-219.
17. Irmiere, A. & Gibson, W. (1983). Isolation and characterization of a noninfectious virion-like particle released from cells infected with human strains of cytomegalovirus. Virology 130, 118-133.
18. Jahn, G., Kouzarides, T., Mach, M., Scholl, B.-C., Plachter, B., Traupe, B., Preddie, E., Satchwell, S. C., Fleckenstein, B. & Barrell, B. G. (1987a). Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. Journal of Virology 61, 1358-1367.
19. Jahn, G., Scholl, B.-C., Traupe, B. & Fleckenstein, B. (1987b). The two major structural phosphoproteins (pp65 and pp150) of human cytomegalovirus and their antigenic properties. Journal of General Virology 68, 1327-1337.
20. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
21. Landini, M. P., Severi, B., Furlini, G. & Badiali De Giorgi, L. (1987). Human cytomegalovirus structural components: intracellular and intraviral localization of p28 and p65-69 by immunoelectron microscopy. Virus Research 8, 15-23.
22. Landini, M. P., Rossier, E. & Schmitz, H. (1988). Antibodies to human cytomegalovirus structural polypeptides during primary infection. Journal of Virological Methods 22, 309-317.
23. Meijer, H., Dreesen, J. C. F. M. & van Boven, C. P. A. (1986). Molecular cloning and restriction endonuclease mapping of the rat cytomegalovirus genome. Journal of General Virology 67, 1327-1342.
24. Meyer, H. H., Ripalti, A., Landini, M. P., Radsak, K., Kern, H. F. & Hensel, G. M. (1997). Human cytomegalovirus late-phase maturation is blocked by stably expressed UL32 antisense mRNA in astrocytoma cells. Journal of General Virology 78, 2621-2631.
25. Mocarski, E. S., Jr (1993). Cytomegalovirus biology and replication. In The Human Herpesviruses, pp. 173-226. Edited by B. Roizman, R. J. Whitley & C. Lopez. New York: Raven Press.
26. Nicholas, J. (1996). Determination and analysis of the complete nucleotide sequence of human herpesvirus 7. Journal of Virology 70, 5975-5989.
27. Rawlinson, W. D., Farrell, H. E. & Barrell, B. G. (1996). Analysis of the complete DNA sequence of murine cytomegalovirus. Journal of Virology 70, 8833-8849.
28. Sanchez, V., Angeletti, P. C., Engler, J. A. & Britt, W. J. (1998). Localization of human cytomegalovirus structural proteins to the nuclear matrix of infected human fibroblasts. Journal of Virology 72, 3321-3329.
29. Schmolke, S., Drescher, P., Jahn, G. & Plachter, B. (1995). Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport. Journal of Virology 69, 1071-1078.
30. Scholl, B.-C, Von Hintzenstern, J., Borisch, B., Traupe, B., Bröker, M. & Jahn, G. (1988). Prokaryotic expression of immunogenic polypeptides of the large phosphoprotein (pp150) of human cytomegalovirus. Journal of General Virology 69, 1195-1204.
31. Spaete, R. R., Gehrz, R. C. & Landini, M. P. (1994). Human cytomegalovirus structural proteins. Journal of General Virology 75, 3287-3308.
32. Stals, F. S., Bosman, F., van Boven, C. P. & Bruggeman, C. A. (1990). An animal model for therapeutic intervention studies of CMV infection in the immunocompromised host. Archives of Virology 114, 91-107.
33. Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 4673-4680.
34. Vink, C., Beuken, E. & Bruggeman, C. A. (1996). Structure of the rat cytomegalovirus genome termini. Journal of Virology 70, 5221-5229.
35. Vink, C., Beuken, E. & Bruggeman, C. A. (1997). Cloning and functional characterization of the origin of lytic-phase DNA replication of rat cytomegalovirus. Journal of General Virology 78, 2963-2973.
36. Zipeto, D., Baldanti, F., Percivalle, E., Gerna, G. & Milanesi, G. (1993). Identification of a human cytomegalovirus mutant in the pp150 matrix phosphoprotein gene with a growth-defective phenotype. Journal of General Virology 74, 1645-1648.