Probing the nature of interactions in SH2 binding interfaces - Evidence from electrospray ionization mass spectrometry

Protein Science

Volumn 8, Issue 10, 1999, Pages 1962-1970

  Chung, Evonne W ^a   Henriques, Denise A ^b   Renzoni, Debora ^b   Morton, Craig J ^a,c   Mulhern, Terrence D ^a,d   Pitkeathly, Maureen C ^a   Ladbury, John E ^b   Robinson, Carol V ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY OF MELBOURNE   (Australia)

^d UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Nanoflow electrospray ionization mass spectrometry; Noncovalent interactions; SH2 domains

Indexed keywords

PEPTIDE; PHOSPHOPEPTIDE; PROTEIN; PROTEIN TYROSINE KINASE; TYROSINE; WATER;

ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION;

EID: 0032849955     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.10.1962     Document Type: Article

References (21)

1. Anderegg RJ, Wagner DS. 1995. Mass spectrometric characterization of a protein-ligand interaction. J Am Chem Soc 117:1374-1377.
2. Chung E, Henriques D, Renzoni D, Zvelebil M, Bradshaw JM, Waksman G, Robinson CV, Ladbury JE. 1998. Mass spectrometric and thermodynamic studies reeveal the role of water molecules in complexes formed between SH2 domains and tyrosyl phosphopeptides. Structure 6:1141-1151.
3. Courtneidge SA, Groutebroze L, Cartwright A, Heber A, Scherneck S, Feunteun J. 1991. Identification and characterization of the hamster polyomavirus middle T antigen. J Virol 65:3301-3308.
4. lck. Nature 368:764-769.
5. Goodford PJ. 1985. A computational approach for determining energetically favourable binding sites on biologically important macromolecules (GRID). J Med Chem 28:849-857.
6. Greig MJ, Gaus H, Cummins LL, Sasmor H, Griffey RH. 1995. Measurement of macromolecular binding using electrospray mass spectrometry. Detection of dissociation constants tor oligonucleotide-serumalbumin complexes. J Am Chem Soc 117:10765-10766.
7. Ladbury JE, Chowdhry BZ. 1996. Sensing the heat: The application of isothermal titration calorimetry to thermodynamic studies of biomolecular interactions. Chem Biol 3:791-801.
8. Ladbury JE, Hensmann M, Panayotou G, Campbell ID. 1996. Alternative modes of tyrosyl phosphopeptide binding to a Src family SH2 domain: Implications for regulation of tyrosine kinase activity. Biochemistry 35:11062-11069.
9. Levitt M, Park BH. 1993. Water: Now you see it, now you don't. Structure 1:223-226.
10. Loo JA, Hu P-F, McConnell P, Mueller WT. 1997. A study of src SH2domain protein-phosphopeptide binding interactions by electrospray ionization mass spectrometry. J Am Soc Mass Spectrom 8:234-243.
11. Mulhern TD, Shaw GL, Morton CJ, Day AJ, Campbell ID. 1997. The SH2 domain from the tyrosine kinase Fyn in complex with a phosphotyrosyl peptide reveals insight into domain stability and binding specificity. Structure 5:1313-1323.
12. Nettleton EJ, Sunde M, Lai Z, Kelly JW, Dobson CM, Robinson CV. 1998. Protein subunit interactions and structural integrity of amyloidogenic transthyretins: Evidence from electrospray mass spectrometry. J Mol Biol 281:553-564.
13. Pintar A, Hensmann M, Jumel K, Pitkeathly M, Harding SE, Campbell ID. 1996. Solution studies of the SH2 domain from the Fyn tyrosine kinase: Secondary structure, backbone dynamics and protein association. Eur Biophys J 24:371-380.
14. Przybylski M, Glocker M. 1996. Electrospray mass spectrometry of biomacromolecular complexes with noncovalent interactions - New analytical perspectives for supramolecular chemistry and molecular recognition processes. Angew Chem Int Ed Engl 35:806-826.
15. Robinson CV, Chung EW, Kragelund BB, Knudsen J, Aplin RT, Poulsen FM, Dobson CM. 1996. Probing the nature of non-covalent interactions by mass spectrometry. A study of protein-CoA ligand binding and assembly. J Am Chem Soc 118:8646-8653.
16. Rostom AA, Sunde M, Richardson SJ, Schreiber G, Jarvis S, Bateman R, Dobson CM, Robinson CV. 1998. Dissection of multi-protein complexes using mass spectrometry - Subunit interactions in transthyretin and retinol-binding protein complexes. Protein Struct Funct Genet 2(Suppl.):3-11.
17. Songyang Z, Shoelson SE, Chaudhuri M, Gish G, Pawson T, Haser WG, Cantley LC. 1993. SH2 domains recognize specific phosphopeptide sequences. Cell 72:767-778.
18. Waksman G, Kominos D, Robertson SC, Pant N, Baltimore D, Birge RB, Cowburn D, Hanafusa H, Mayer BJ, Overduin M. et al. 1992. Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides. Nature 358:646-653.
19. Waksman G, Shoelson SE, Pant N, Cowburn D, Kuriyan J. 1993. Binding of a high affinity phosphotyrosyl peptide to the src SH2 domain: Crystal structures of the complexed and peptide-free forms. Cell 72:779-790.
20. Wilm MS, Mann M. 1994. Electrospray and Taylor-Cone theory, Dole's beam of macromolecules at last? Int J Mass Spectrom Ion Process 136:167-180.
21. Wu Q-Y, Gao J-M, Joseph-McCarthy D, Sigal GB, Bruce E, Whitesides GM, Smith RD. 1997. Carbonic anhydrase-inhibitor binding: From solution to the gas phase. J Am Chem Soc 119:1157-1158.