Competitive RT-PCR for measuring metalloproteinase gene expression in human mesangial cells exposed to a hyperglycemic environment

BioTechniques

Volumn 27, Issue 3, 1999, Pages 512-520

  Death, A K ^a   Yue, D K ^a   Turtle, J R ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

METALLOPROTEINASE;

ARTICLE; DIABETES MELLITUS; DIABETIC NEPHROPATHY; DNA TEMPLATE; GENE EXPRESSION; GLUCOSE BLOOD LEVEL; HUMAN; HUMAN CELL; MESANGIUM CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0032849144     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99273st05     Document Type: Article

References (11)

1. Andel Wahab, N. and R.M. Mason. 1996. Modulation of neutral protease expression in human mesangial cells by hyperglycaemic culture. Biochem. J. 320:777-783.
2. Connolly, A.R., L.G. Cleland and B.W. Kirkham. 1995. Mathematical considerations of competitive polymerase chain reaction. J. Immunol. Methods 187:201-211.
3. Lum, Z.P., B.E. Hakala, J.S. Mort and A.D. Recklies. 1996. Modulation of the catabolic effects of interleukin-1β on human articular chondrocytes by transforming growth factor-β. J. Cell Physiol. 166:351-359.
4. McCulloch, R.K., C.S. Choong and D.M. Hurley. 1995. An evaluation of competitor type and size for use in the determination of mRna by competitive PCR. PCR Methods Appl. 4:219-226.
5. McLennan, S.V., E.J. Fisher, D.K. Yue and J.R. Turtle. 1994. High glucose concentration causes a decrease in mesangium degradation: a factor in the pathogenesis of diabetic nephropathy. Diabetes 43:1041-1045.
6. McLennan, S.V., D.K. Yue and J.R. Turtle. 1998. The effect of glucose on matrix metalloproteinase activity in mesangial cells. Nephron 79:293-299.
7. Melhus, H., A. Gobl and S. Ljunghall. 1994. Competitive PCR demonstrates that 9-cis retinoic acid induces cellular retinoic acid-binding protein-II more efficiently than all-trans retinoic acid in human osteosarcoma cells. Biochem. Biophys. Res. Commun. 200:1125-1129.
8. Menzo, S., P. Bannarelli, M. Giacca, A. Manzin, P.E. Varaldo and M. Clementi. 1992. Absolute quantitation of viremia in human imunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction. J. Clin. Microbiol. 30:1752-1757.
9. Ross, R., R. Kleiz and A.B. Reske-Kunz. 1995. A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions. PCR Methods Appl. 4:371-375.
10. Schmidt, D.M. and J.D. Ernst. 1995. A fluorometric assay for the quantification of RNA in solution with nanogram sensitivity. Anal. Biochem. 232:144-146.
11. Yao, P.M., J.M. Buhler, M.P. d'Ortho, F. Lenargy, C. Delclaux, A. Harf and C. Lafume. 1996. Expression of matrix metalloproteinase gelatinases A and B by cultured epithelial cells from human bronchial explants. J. Biol. Chem. 271:15880-15589.