Purification of Aspergillus sp xylanase by precipitation with an anionic polymer Eudragit S100

Process Biochemistry

Volumn 34, Issue 6-7, 1999, Pages 577-580

  Gawande, P V ^a   Kamat, M Y ^b  

^a Sr Res Fellow Univ Grant Commn   (India)

^b INSTITUTE OF CHEMICAL TECHNOLOGY   (India)

Author keywords

Affinity precipitation; Anionic polymer; Aspergillus sp; Enzyme purification; Eudragit S100; Xylanase

Indexed keywords

EUDRAGIT; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; ASPERGILLUS; BIOPROCESS; ENZYME PURIFICATION; ENZYME SYNTHESIS; NONHUMAN; PRECIPITATION;

EID: 0032844145     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(98)00123-X     Document Type: Article

References (19)

1. Bastawde K.B. Xylan structure, microbial xylanase, and their mode of action. World J Microbiol Biotechnol. 8:1992;353-368.
2. Wong K.K.Y., Saddler J.N. Multiplicity of β-1,4-xylanase in microorganisms: functions and applications. Microbiol Rev. 52:1988;305-317.
3. Biely P. Microbial xylanolytic systems. Trends Biotechnol. 3:1985;286-290.
4. Haltrich D., Nidetzky B., Kulbe K.D., Steiner W., Zupancic S. Production of fungal xylanases. Biores Technol. 58:1996;137-161.
5. Gawande P.V., Kamat M.Y. Immobilization of Aspergillus sp on nylon bolting cloth for production of xylanase. J. Ferment. Bioeng. 2:1998;243-246.
6. Cesar T., Mrsa V. Purification and properties of the xylanase produced by Thermomyces lanuginous. Enzym Microbiol Technol. 19:1996;289-296.
7. Fujimoto H., Ooi T., Wahg S.L., Takizawa T., Hidaka H., Murao S., Arai M. Purification and properties of three xylanase from Aspergillus aculeatus. Biosci Biotechnol Biochem. 59:1995;538-540.
8. Galaev I.Y., Gupta M. N., Mattiasson B. Use smart polymers for bioseparations. Chemtech. 12:1996;19-25.
9. Schneider M., Guillot C., Lamy B. The affinity precipitation technique. Application to the isolation and purification of trypsin from bovine pancreas. Annals of the NY Academ Sci. 369:1981;257-263.
10. Guoquiang D., Batra R., Kaul R., Gupta M.N., Mattiassion B. Alternative modes of precipitation of Eudragit S 100: a potential ligand carrier for affinity precipitation of protein. Bioseparation. 5:1995;339-350.
11. Mandels M., Sternberg D. Recent advances in cellulase technology. J Ferment Technol. 54:1976;267-286.
12. Gupta M.N., Guogiang D., Kaul R., Mattiasson B. Purification of xylanase from Trichoderma viride by precipitation with an anioinc polymer Eudragit S100. Biotechnol Tech. 8:1994;117-122.
13. Miller L.G. Use of dinitrosalycilic acid reagent for determination of reducing sugar. Analyt Chem. 31:1959;426-428.
14. Lowry O.H., Rosebriugh N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J Biol Chem. 193:1959;265-275.
15. Laemmli UK. Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature. 277:1970;680-685.
16. Schwarz W.H., Bronnenmeier K., Gräbnitz F., Staudenbauer W. L. Activity staining of cellulase in polyacrylamide gels containing mixed linkage β-glucans. Analyt Biochem. 164:1987;72-77.
17. Tyagi R., Gupta M.N. Immobilization of Aspergillus niger xylanase on magnetic latex beads. Biotechnol Appl Biochem. 21:1995;217-222.
18. Lopez C., Blanco A., Pastor F.l.J. Xylanase production by a new alkali-tolerant isolate of Bacillus. Biotechnol Lett. 20:1998;243-246.
19. Chandra R.K., Chandra T.S. Purification and characterization of xylanase from alkali-tolerant Aspergillus fischeri Fxn 1. FEMS Microbiol Lett. 145:1996;457-461.