Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence

Journal of Clinical Microbiology

Volumn 34, Issue 8, 1996, Pages 1885-1891

  Erhardt, Andreas ^a   Schaefer, Stephan ^a   Athanassiou, Nikoleta ^a   Kann, Michael ^a   Gerlich, Wolfram H ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DIGOXIGENIN; ETHIDIUM BROMIDE; STREPTAVIDIN; VIRUS DNA;

ARTICLE; CHEMOLUMINESCENCE; COLORIMETRY; DNA HYBRIDIZATION; DNA PROBE; HEPATITIS B VIRUS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRUS DETECTION;

HEPATITIS B VIRUS;

EID: 0029890734     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.8.1885-1891.1996     Document Type: Article

References (37)

1. Barlet, V., J. P. Zarski, M. A. Thelu, and J. M. Seigneurin. 1991. Advantage of PCR for detecting low amounts of HBV DNA in patients' sera. Res. Virol. 142:373-379.
2. Berninger, M., M. Hammer, B. Hoyer, and J. L. Gerin. 1982. An assay for the detection of the DNA genome of hepatitis B virus in serum. J. Med. Virol. 9:57-68.
3. Bronstein, I., and L. Kricka. 1989. Clinical applications of luminescent assays for enzymes and enzyme labels. J. Clin. Lab. Anal. 3:316-322.
4. Carman, W. F., M. R. Jacyna, S. Hadziyannis, P. Karayiannis, M. Mc-Garvey, A. Makris, and H. C. Thomas. 1989. Mutation preventing formation of e antigen in patients with chronic HBV infection. Lancet 336:588-591.
5. Chazouillières, O., D. Mamish, M. Kim, K. Carey, L. Ferrell, J. R. Roberts, N. L. Ascher, and T. L. Wright. 1994. "Occult" hepatitis B virus as a source of infection in liver transplant recipients. Lancet 343:142-146.
6. Dulbecco, R. 1980. The nature of viruses, p. 853-884. In B. D. Davis, R. Dulbecco, H. N. Eisen, and H. S. Ginsberg (ed.), Microbiology, 3rd ed. Harper and Row Publishers Inc., Philadelphia.
7. Durrant, I. 1990. Light-based detection of biomolecules. Nature (London) 346:297-298.
8. Escarceller, M., F. Rodriguez-Frias, R. Jardi, B. San Segundo, and R. Eritja. 1992. Detection of hepatitis B virus DNA in human serum samples: use of digoxigenin-labelled oligonucleotides as modified primers for the polymerase chain reaction. Anal. Biochem. 206:36-42.
9. Fox, J. C., and V. C. Emery. 1992. Quantification of viruses in clinical samples. Rev. Med. Virol. 2:195-203.
10. Garcia, F., F. Garcia, M. C. Bernal, A. Leyla, G. Piedrola, and M. C. Maroto. 1995. Evaluation of enzyme immunoassay for hepatitis B virus DNA based on anti-double-stranded DNA. J. Clin. Microbiol. 33:413-415.
11. Gerken, G., P. Paterlini, M. Manns, C. Housset, S. Terre, H. P. Dienes, G. Hess, W. H. Gerlich, P. Berthelot, and K. H. Meyer zum Büschenfelde. 1991. Assay of hepatitis B virus DNA by polymerase chain reaction and its relationship to pre-S- and S-encoded viral surface antigens. Hepatology 13:158-166.
12. Gerlich, W. H., K. H. Heermann, R. Thomssen, and the Eurohep Group. 1995. Quantitative assays for hepatitis B virus DNA: standardization and quality control. Virol. Hepatitis Rev. 1:53-57.
13. Heermann, K. H., Y. Hagos, and R. Thomssen. 1994. Liquid-phase hybridization and capture of hepatitis B virus DNA with magnetic beads and fluorescence detection of PCR product. J. Virol. Methods 50:43-57.
14. Hoofnagle, J. H., and A. M. Di Bisceglie. 1991. Serologic diagnosis of acute and chronic viral hepatitis. Semin. Liver Dis. 11:73-83.
15. Jalava, T., P. Lehtovaara, A. Kallio, M. Ranki, and H. Söderlund. 1993. Quantification of hepatitis B virus DNA by competitive amplification and hybridization on microplates. BioTechniques 15:134-139.
16. Jenison, S. A., S. M. Lemon, L. N. Baker, and J. E. Newbold. 1987. Quantitative analysis of hepatitis B virus DNA in saliva and semen of chronically infected homosexual men. J. Infect. Dis. 156:299-307.
17. Kaneko, S., R. H. Miller, A. M. Di Bisceglie, S. M. Feinstone, J. H. Hoofnagle, and R. H. Purcell. 1990. Detection of hepatitis B virus DNA in serum by polymerase chain reaction. Gastroenterology 99:799-804.
18. Kaneko, S., R. H. Miller, S. M. Feinstone, M. Unoura, K. Kobayashi, N. Hattori, and R. H. Purcell. 1989. Detection of hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. Proc. Natl. Acad. Sci. USA 86:312-316.
19. Keller, G. H., D. P. Huang, J. W. Shih, and M. M. Manak. 1990. Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization. J. Clin. Microbiol. 28:1411-1416.
20. Kuhns, M. C., A. L. McNamara, R. P. Perrillo, C. M. Cabal, and C. R. Campbell. 1989. Quantitation of hepatitis B viral DNA by solution hybridization: comparison with DNA polymerase and hepatitis B e antigen during antiviral therapy. J. Med. Virol. 27:274-281.
21. Larzul, D., D. Chevrier, and J. L. Guesdon. 1989. A non-radioactive diagnostic test for the detection of HBV DNA sequences in serum at the single molecule level. Mol. Cell. Probes 3:45-57.
22. Matthews, J. A., A. Batki, C. Hynds, and L. J. Kricka. 1985. Enhanced chemiluminescent method for the detection of DNA dot-hybridization assays. Anal. Biochem. 151:205-209.
23. Paterlini, P., G. Gerken, E. Nakajima, S. Terre, A. D'Errico, W. Grigioni, B. Nalpas, D. Franco, J. Wands, M. Kew, et al. 1990. Polymerase chain reaction to detect hepatitis B virus DNA and RNA sequences in primary liver cancers from patients negative for hepatitis B surface antigen. N. Engl. J. Med. 323:80-85.
24. Quint, W. G. V., R. A. Heijtink, J. Schirm, W. H. Gerlich, and H. G. M. Niesters. 1995. Reliability of methods for hepatitis B virus DNA detection. J. Clin. Microbiol. 33:225-228.
25. Renz, M., and C. Kurz. 1984. A colorimetric method for DNA hybridization. Nucleic Acids Res. 12:3435-3444.
26. Repp, R., S. Rhiel, K. H. Heermann, S. Schaefer, C. Keller, P. Ndumbe, F. Lampert, and W. H. Gerlich. 1993. Genotyping by multiplex polymerase chain reaction for detection of endemic hepatitis B virus transmission. J. Clin. Microbiol. 31:1095-1102.
27. Schaefer, S. Unpublished data.
28. Scotto, J., M. Hadchouel, C. Hery, J. Yvart, P. Tiollais, and C. Bréchot. 1983. Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: comparison with results for other viral markers. Hepatology 3:279-284.
29. Ulrich, P. P., R. A. Ramesh, B. Seto, D. Mack, J. Sninsky, and G. N. Vyas. 1989. Enzymatic amplification of hepatitis B virus DNA in serum compared to infectivity testing in chimpanzees. J. Infect. Dis. 160:37-43.
30. Urdea, M. S., T. Horn, T. J. Fultz, M. Anderson, J. A. Running, S. Hamren, D. Ahle, and C. Chang. 1991. Branched DNA multimers for the sensitive, direct detection of human hepatitis viruses. Nucleic Acids Res. 24:197-200.
31. Urdea, M. S., B. D. Warner, J. A. Running, M. Stempien, J. Clyne, and T. Horn. 1988. A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes. Nucleic Acids Res. 16:4937-4956.
32. Valentine-Thon, E. 1995. Evaluation of Sharp signal system for enzymatic detection of amplified hepatitis B virus DNA. J. Clin. Microbiol. 33:477-480.
33. Valenzuela, P., M. Quiroga, J. Zaldivar, P. Gray, and W. J. Rutter. 1980. The nucleotide sequence of the viral hepatitis B genome and the identification of the major viral genes, p. 57-70. In B. N. Fields, R. Jaenisch, and C. F. Fox (ed.), Animal virus genetics. Academic Press, Inc., New York.
34. Yang, G., P. P. Ulrich, R. A. Aiyer, B. D. Rawal, and G. N. Vyas. 1993. Detection of hepatitis B virus in plasma using flow cytometric analyses of polymerase chain reaction-amplified DNA incorporating digoxigenin-11-dUTP. Blood 81:183-188.
35. Zaaijer, H. L., F. ter-Borg, H. T. Cuypers, M. C. Hermus, and P. N. Lelie. 1994. Comparison of methods for detection of hepatitis B virus DNA. J. Clin. Microbiol. 32:2088-2091.
36. Zarski. J. P., M. Kuhns, L. Berck, F. Degos, S. W. Schalm, P. Tiollais, and C. Bréchot. 1989. Comparison of a quantitative standardized HBV-DNA assay and a classical spot hybridization test in chronic active hepatitis B patients undergoing antiviral therapy. Res. Virol. 140:283-291.
37. Zyzik, E., W. H. Gerlich, A. Uy, H. Köchel, and R. Thomssen. 1986. Assay of hepatitis B virus genome titers in sera of infected subjects. Eur. J. Clin. Microbiol. 5:330-335.