Monomer-trimer equilibrium of the ectodomain of SIV gp41: Insight into the mechanism of peptide inhibition of HIV infection

Protein Science

Volumn 8, Issue 9, 1999, Pages 1904-1907

  Caffrey, Michael ^a   Kaufman, Joshua ^b   Stahl, Stephen ^b   Wingfield, Paul ^b   Gronenborn, Angela M ^a   Clore, G Marius ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES   (United States)

Author keywords

gp41; HIV; Monomer; Peptide inhibition; SIV; Trimer

Indexed keywords

CARBON; CARBON 13; GLYCOPROTEIN GP 41;

ARTICLE; CIRCULAR DICHROISM; EQUILIBRIUM CONSTANT; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN DOMAIN; SIMIAN IMMUNODEFICIENCY VIRUS; ULTRACENTRIFUGATION;

HUMAN IMMUNODEFICIENCY VIRUS; SIMIAE; SIMIAN IMMUNODEFICIENCY VIRUS;

EID: 0032843108     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.9.1904     Document Type: Article

References (17)

1. Blacklow S, Lu M, Kim P. 1995. A trimeric subdomain of the simian immunodeficiency virus envelope glycoprotein. Biochemistry 34:14955-14962.
2. Caffrey M, Cai M, Kaufman J, Stahl SJ, Wingfield PT, Gronenborn AM, Clore GM. 1997. Determination of the secondary structure and global topology of the 44 kDa ectodomain of gp41 of the simian immunodeficiency virus by multidimensional nuclear magnetic resonance spectroscopy. J Mol Biol 271:819-826.
3. Caffrey M, Cai M, Kaufman J, Stahl S, Wingfield P, Covell D, Gronenborn AM, Clore GM. 1998a. Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41. EMBO J 17:4572-4584.
4. 15N relaxation data in large proteins: Application to the 44 kDa ectodomain of SIV gp41. J Magn Reson 135:368-372.
5. Chan DC, Fass D, Berger JM, Kim PS. 1997. Core structure of gp41 from the HIV envelope glycoprotein. Cell 89:263-273.
6. Clore GM, Gronenborn AM. 1998. Determining the structures of large proteins and protein complexes by NMR. Trends Biotech 16:22-34.
7. Frankel AD, Young JA. 1998. HIV-1: Fifteen proteins and an RNA. Ann Rev Biochem 67:1-25.
8. Kilby JM, Hopkins S, Venetta TM, DiMassimo B, Cloud GA, Lee JY, Alldredge L, Hunter E, Lambert D, Bolognesi D. 1998. Potent suppression of HIV-1 replication in humans by T-20, a peptide inhibitor of gp41-mediated virus entry. Nat Medicine 4:1302-1307.
9. Lu M, Blacklow S, Kim P. 1995. A trimeric structural domain of the HIV-1 transmembrane glycoprotein. Nature Struct Biol 2:1075-1082.
10. Malashkevich VN, Chan DC, Chutowski CT, Kim PS. 1998. Crystal structure of the simian immunodeficiency virus (SIV) gp41 core: Conserved helical interactions underlie the broad inhibitory activity of gp41 peptides. Proc Natl Acad Sci USA 95:9134-9139.
11. McRorie DK, Voelker PJ. 1993. Publication 362784 Beckman Instruments Inc., Fullerton, California.
12. Tan K, Liu JH, Wang JH, Shen S, Lu M. 1997. Atomic structure of a thermostable subdomain of HIV-1 gp41. Proc Natl Acad Sci USA 94:12203-12308.
13. Weissenhorn W, Dessen A, Harrison SC, Skehel JJ, Wiley DC. 1997. Atomic structure of the ectodomain from HIV-1 gp41. Nature (London) 387:426-430.
14. Weissenhorn W, Wharton S, Calder L, Earl P, Moss B, Aliprandis E, Skehel JJ, Wiley D. 1996. The ectodomain of HIV-1 envelope subunit gp41 forms a soluble, α-helical, rod-like oligomer in the absence of gp120 and the N-terminal fusion peptide. EMBO 15:1507-1514.
15. Wild CT, Oas T, McDanal CB, Bolognesi D, Matthews TJ. 1992. A synthetic peptide inhibitor of human immunodeficiency virus replication: Correlation between solution structure and viral inhibition. Proc Natl Acad Sci USA 89:10537-10541.
16. Wild CT, Shugars DC, Greenwell TK, McDanal CB, Matthews TJ. 1994. Peptides corresponding to a predictive α-helical domain of human immunodeficiency virus type 1 gp41 are potent inhibitors of virus infection. Proc Natl Acad Sci USA 91:9770-9774.
17. Wingfield PT, Stahl SJ, Kaufman J, Zlotnick A, Hyde CC, Gronenborn AM, Clore GM. 1997. The extracellular domain of immunodeficiency virus gp41 protein. Expression in Escherichia coli, purification, and crystallization. Protein Sci 6:1653-1660.