A conserved acidic motif in the N-terminal domain of nitrate reductase is necessary for the inactivation of the enzyme in the dark by phosphorylation and 14-3-3 binding

Plant Physiology

Volumn 119, Issue 1, 1999, Pages 219-229

  Pigaglio, Emmanuelle ^a,b   Durand, Nathalie ^a   Meyer, Christian ^a  

^a CEMAGREF   (France)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; CASEIN KINASE II; DELETION MUTAGENESIS; ENZYME INACTIVATION; ENZYME STRUCTURE; NICOTIANA PLUMBAGINIFOLIA; NITRATE REDUCTASE; PHOSPHORYLATION; PROMOTER REGION;

BRASSICA OLERACEA VAR. BOTRYTIS; CAULIFLOWER MOSAIC VIRUS; EMBRYOPHYTA; NICOTIANA PLUMBAGINIFOLIA; NICOTIANA TABACUM; TABACUM;

EID: 0032840889     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.119.1.219     Document Type: Article

References (48)

1. Bachmann M, Huber JL, Liao P-C, Gage DA, Huber SC (1996a) The inhibitor protein of phosphorylated nitrate reductase from spinach (Spinacia oleracea) leaves is a 14-3-3 protein. FEBS Lett 387: 127-131
2. Bachmann M, Shiraishi N, Campbell WH, Yoo B-C, Harmon AC, Huber SC (1996b) Identification of Ser-543 as the major regulatory phosphorylation site in spinach leaf nitrate reductase. Plant Cell 8: 505-517
3. Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12: 8711-8721
4. Bullock WO, Fernandez JM, Short JM (1987) XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Biotechniques 5: 376-378
5. Campbell WH (1996) Nitrate reductase biochemistry comes of age. Plant Physiol 111: 355-361
6. Chen B, Przybyla AE (1994) An efficient site-directed mutagenesis method based on PCR. Biotechniques 17: 657-659
7. Coïc Y, Lesaint C (1975) La nutrition minérale et en eau des plantes en horticulture avancée. Doc Tech Commerc Potasses Alsace 23: 1-22
8. Crawford NM (1995) Nitrate: nutrient and signal for plant growth. Plant Cell 7: 859-868
9. Crété P, Caboche M, Meyer C (1997) Nitrite reductase expression is regulated at the post-transcriptional level by the nitrogen source in Nicotiana plumbaginifolia and Arabidopsis thaliana. Plant J 11: 625-634
10. Davies SP, Carling D, Hardie DG (1989) Tissue distribution of the AMP-activated protein kinase and lack of activation by cyclic-AMP-dependent protein kinase, studied using a specific and sensitive peptide assay. Eur J Biochem 186: 123-128
11. 2 fixation in the control of nitrate-reductase activity in barley leaves. Planta 190: 277-283
12. Douglas P, Morrice N, MacKintosh C (1995) Identification of a regulatory phosphorylation site in the hinge 1 region of nitrate reductase from spinach (Spinacea oleracea) leaves. FEBS Lett 377: 113-117
13. 2+ ions. Biochem J 325: 101-109
14. Gabard J, Marion-Poll A, Chérel I, Meyer C, Müller AJ, Caboche M (1987) Isolation and characterization of Nicotiana plumbaginifolia nitrate reductase-deficient mutants: genetic and biochemical analysis of the NIA complementation group. Mol Gen Genet 209: 596-606
15. Glaab J, Kaiser WM (1993) Rapid modulation of nitrate reductase in pea roots. Planta 191: 173-179
16. Glaab J, Kaiser WM (1995) Inactivation of nitrate reductase involves NR-protein phosphorylation and subsequent 'binding' of an inhibitor protein. Planta 195: 514-518
17. Hoff T, Truong H-N, Caboche M (1994) The use of mutants and transgenic plants to study nitrate assimilation. Plant Cell Environ 17: 489-506
18. Huber JL, Huber SC, Campbell WH, Redinbaugh MG (1992) Reversible light/dark modulation of spinach leaf nitrate reductase activity involves protein phosphorylation. Arch Biochem Biophys 296: 58-65
19. Huber JL, Redinbaugh MG, Huber SC, Campbell WH (1994) Regulation of maize leaf nitrate reductase activity involves both gene expression and protein phosphorylation. Plant Physiol 106: 1667-1674
20. Kaiser WM, Brendle-Behnisch E (1991) Rapid modulation of spinach leaf nitrate reductase activity by photosynthesis. II. In vitro modulation by ATP and AMP. Plant Physiol 96: 368-375
21. 2 prevents nitrate reduction in leaves. Plant Physiol 91: 970-974
22. Kaiser WM, Huber SC (1994a) Posttranslational regulation of nitrate reductase in higher plants. Plant Physiol 106: 817-821
23. 2+ and 5′-AMP. Planta 193: 358-364
24. Kaiser WM, Huber SC (1997) Correlation between apparent activation state of nitrate reductase (NR), NR hysteresis and degradation of NR protein. J Exp Bot 48: 1367-1374
25. Kaiser WM, Spill D, Brendle-Behnisch E (1992) Adenine nucleotides are apparently involved in the light-dark modulation of spinach-leaf nitrate reductase. Planta 186: 236-240
26. Kaiser WM, Spill D, Glaab J (1993) Rapid modulation of nitrate reductase in leaves and roots: indirect evidence for the involvement of protein phosphorylation/dephosphorylation. Physiol Plant 89: 557-562
27. Kojima M, Wu S-J, Fukui H, Sugimoto T, Nanmori T, Oji Y (1995) Phosphorylation/dephosphorylation of Komatsuna (Brassica campestris) leaf nitrate reductase in vivo and in vitro in response to environmental light conditions: effects of protein kinase and protein phosphatase inhibitors. Physiol Plant 93: 139-145
28. LaBrie S, Crawford NM (1994) A glycine to aspartic acid change in the MoCo domain of nitrate reductase reduces both activity and phosphorylation levels in Arabidopsis. J Biol Chem 269: 14497-14501
29. Li XZ, Oaks A (1994) Induction and turnover of nitrate reductase in Zea mays. Influence of light. Plant Physiol 106: 1145-1149
30. Lillo C (1993) Magnesium and calcium inhibition of squash leaf NADH nitrate reductase. Plant Cell Physiol 34: 1181-1185
31. Lillo C, Kazazaic S, Ruoff P, Meyer C (1997) Characterization of nitrate reductase from light- and dark-exposed leaves. Plant Physiol 114: 1377-1383
32. Lin R, Beauparlant P, Makris C, Meloche S, Hiscott J (1996) Phosphorylation of IκBα in the C-terminal PEST domain by casein kinase II affects protein stability. Mol Cell Biol 16: 1401-1409
33. MacKintosh C (1992) Regulation of spinach-leaf nitrate reductase by reversible phosphorylation. Biochim Biophys Acta 1137: 121-126
34. MacKintosh C, Douglas P, Lillo C (1995) Identification of a protein that inhibits the phosphorylated form of nitrate reductase from spinach (Spinacia oleracea) leaves. Plant Physiol 107: 451-457
35. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
36. Meyer C, Gonneau M, Caboche M, Rouzé P (1995) Identification by mutational analysis of four critical residues in the molybdenum cofactor domain of eukaryotic nitrate reductase. FEBS Lett 370: 197-202
37. Moorhead G, Douglas P, Morrice N, Scarabel M, Aitken A, MacKintosh C (1996) Phosphorylated nitrate reductase from spinach leaves is inhibited by 14-3-3 proteins and activated by fusicoccin. Curr Biol 6: 1104-1113
38. Nussaume L, Vincentz M, Meyer C, Boutin JP, Caboche M (1995) Post-transcriptional regulation of nitrate reductase by light is abolished by an N-terminal deletion. Plant Cell 7: 611-621
39. Pearson RB, Kemp BE (1991) Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations. Methods Enzymol 200: 62-81
40. Rechsteiner M, Rogers SW (1996) PEST sequences and regulation by proteolysis. Trends Biochem Sci 21: 267-271
41. Rost B, Sander C (1994) Combining evolutionary information and neural networks to predict protein secondary structure. Proteins 19: 55-72
42. Schwarz EM, van Antwerp D, Verma IM (1996) Constitutive phosphorylation of IκBα by casein kinase II occurs preferentially at serine 293: requirement for degradation of free IκBα. Mol Cell Biol 16: 3554-3559
43. Spill D, Kaiser WM (1994) Partial purification of two proteins (100 kDa, 67 kDa) cooperating in the ATP-dependent inactivation of spinach leaf nitrate reductase. Planta 192: 183-188
44. Su W, Huber SC, Crawford NM (1996) Identification in vitro of a post-translarional regulatory site in the hinge 1 region of Arabidopsis nitrate reductase. Plant Cell 8: 519-527
45. Vaucheret H, Chabaud M, Kronenberger J, Caboche M (1990) Functional complementation of tobacco and Nicotiana plumbaginifolia nitrate reductase deficient mutants by transformation with the wild-type allele of the tobacco structural gene. Mol Gen Genet 220: 468-474
46. Vaucheret H, Vincentz M, Kronenberger J, Caboche M, Rouzé P (1989) Molecular cloning and characterization of the homologous genes coding for nitrate reductase in tobacco. Mol Gen Genet 216: 10-15
47. Vincentz M, Caboche M (1991) Constitutive expression of nitrate reductase allows normal growth and development of Nicotiana plumbaginifolia plants. EMBO J 10: 1027-1035
48. Vincentz M, Moureaux T, Leydecker M-T, Vaucheret H, Caboche M (1993) Regulation of nitrate and nitrite reductase expression of Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites. Plant J 3: 315-324