Purification and ligand binding of EmrR, a regulator of a multidrug transporter

Journal of Bacteriology

Volumn 181, Issue 16, 1999, Pages 5131-5133

  Brooun, Alexei ^a   Tomashek, John J ^a   Lewis, Kim ^a  

^a TUFTS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,4 DINITROPHENOL; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; GLYCOPROTEIN P;

ARTICLE; CONTROLLED STUDY; EQUILIBRIUM DIALYSIS; ESCHERICHIA COLI; LIGAND BINDING; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REPRESSOR GENE;

EID: 0032839007     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.16.5131-5133.1999     Document Type: Article

References (21)

1. Ahmed, M., C. M. Borsch, S. S. Taytor, N. Vazquez-Laslop, and A. A. Neyfakh. 1994. A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates. J. Biol. Chem. 269: 28505-28513.
2. Clifton, J., A. Brooun, K. Lewis, and G. Petsko. Unpublished data.
3. Dalrymple, B. P., and Y. Swadling. 1997. Expression of a Butyrivibrio fibrisolvens E14 gene (cinB) encoding an enzyme with cinnamoyl ester hydrolase activity is negatively regulated by the product of an adjacent gene (cinR). Microbiology 143:1203-1210.
4. del Castillo, I., J. E. Gonzalez-Pastor, J. L. San Milian, and F. Moreno. 1991. Nucleotide sequence of the Escherichia coli regulatory gene mprA and construction and characterization of mprA-deficient mutants. J. Bacteriol. 173: 3924-3929.
5. Furukawa, H., J. T. Tsay, S. Jackowski, Y. Takamura, and C. O. Rock. 1993. Thiolactomycin resistance in Escherichia coli is associated with the multidrug resistance efflux pump encoded by emrAB. J. Bacteriol. 175:3723-3729.
6. Grkovic, S., M. H. Brown, N. J. Roberts, I. T. Paulsen, and R. A. Skurray. 1998. QacR is a represser protein that regulates expression of the Staphylococcus aureus multidrug efflux pump QacA. J. Biol. Chem. 273:18665-18673.
7. Levy, S. B. 1998. The challenge of antibiotic resistance. Sci. Am. 278:46-53.
8. Lewis, K. 1994. Multidrug resistance pumps in bacteria: variations on a theme. Trends Biochem. Sci. 19:119-123.
9. Lewis, K. 1999. Multidrug resistance: versatile drug sensors of bacterial cells. Curr. Biol. 9:R403-R407.
10. Lomovskaya, O., F. Kawai, and A. Matin. 1996. Differential regulation of the mcb and emr operons of Escherichia coli: role of mcb in multidrug resistance. Antimicrob. Agents Chemother. 40:1050-1052.
11. Lomovskaya, O., and K. Lewis. 1992. Emr, an Escherichia coli locus for multidrug resistance. Proc. Natl. Acad. Sci. USA 89:8938-8942.
12. Lomovskaya, O., K. Lewis, and A. Matin. 1995. EmrR is a negative upstream regulator of the E. coli multidrug resistance pump EmrAB. J. Bacteriol. 177:2328-2334.
13. Mao, W., and D. A. Siegele. 1998. Genetic analysis of the stationary phase-induced mcb operon promoter in Escherichia coli. Mol. Microbiol. 27:415-424.
14. Markham, P. N., M. Ahmed, and A. A. Neyfakh. 1996. The drug-binding activity of the multidrug-responding transcriptional regulator BmrR resides in its C-terminal domain. J. Bacteriol. 178:1473-1475.
15. Martin, R G., K. W. Jair, R. E. Wolf, Jr., and J. L. Rosner. 1996. Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli. J. Bacteriol. 178:2216-2223.
16. Martin, R. G., and J. L. Rosner. 1995. Binding of purified multiple antibiotic-resistance represser protein (MarR) to mar operator sequences. Proc. Natl. Acad. Sci. USA 92:5456-5460.
17. Miller, P. F., and M. C. Sulavik. 1996. Overlaps and parallels in the regulation of intrinsic multiple-antibiotic resistance in Escherichia coli. Mol. Microbiol. 21:441-448.
18. Nikaido, H. 1998. Multiple antibiotic resistance and efflux. Curr. Opin. Microbiol. 1:516-523.
19. Paulsen, I. T., M. H. Brown, and R. A. Skurray. 1996. Proton-dependent multidrug efflux systems. Microbiol. Rev. 60:575-608.
20. Seoane, A. S., and S. B. Levy. 1995. Characterization of MarR, the repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli. J. Bacteriol. 177:3414-3419.
21. Zheleznova, E. A., P. N. Markham, A. A. Neyfakh, and R. G. Brennan. 1999. Structural basis of multidrug recognition by BmrR, a transcription activator of a multidrug transporter. Cell 96:353-362.