A nonessential signal peptidase II (Lsp) of Myxococcus xanthus might be involved in biosynthesis of the polyketide antibiotic TA

Journal of Bacteriology

Volumn 181, Issue 18, 1999, Pages 5644-5651

  Paitan, Yossi ^a   Orr, Elisha ^b   Ron, Eliora Z ^a   Rosenberg, Eugene ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL DNA; GLOBOMYCIN; GLUCAN SYNTHASE; POLYKETIDE; PROTEINASE; SIGNAL PEPTIDASE II;

ANTIBIOTIC BIOSYNTHESIS; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENE CLUSTER; GENE MAPPING; GENE OVEREXPRESSION; HYDROPHOBICITY; MYXOCOCCALES; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SOIL MICROFLORA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MYXOCOCCALES; MYXOCOCCUS XANTHUS; NEGIBACTERIA;

EID: 0032836762     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.18.5644-5651.1999     Document Type: Article

References (52)

1. Albertini, A. M., T. Caramori, F. Scoffone, C. Scotti, and A. Galizzi. 1995. Sequence around the 159° region of the Bacillus subtilis genome: the pksX locus spans 33.6-kb. Microbiology 141:299-309.
2. Altschul, S. F., W. Gish, W. Miller, W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 251:403-410.
3. Avery, L., and D. Kaiser. 1983. In situ transposon replacement and isolation of spontaneous tandem duplication. Mol. Gen. Genet. 191:99-109.
4. Braun, V., and K. Rehn. 1969. Chemical characterization, spatial distribution and function of lipoproteins (murein lipoprotein) of E. coli cell wall. The specific effect of trypsin on membrane structure. Eur. J. Biochem. 10:423-438.
5. Fleischmann, R. D., M. D. Adams, O. White, et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
6. Fraser, C. M., J. D. Gocayne, O. White, et al. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
7. Genetics Computer Group. 1991. Sequence analysis software package, version 7. University of Wisconsin Genetics Computer Group, Madison.
8. Guespin-Michel, J., B. Letouvet-Pawlak, and F. Petit. 1993. Protein secretion in myxobacteria, p. 235-255. In M. Dworkin and D. Kaiser (ed.), Myxobacteria II. American Society for Microbiology, Washington, D.C.
9. Guespin-Michel, J. Personal communication.
10. Hanlon, W. A., M. Martinez-Canamero, M. Inouye, and S. Inouye. 1995. MIpA, a lipoprotein required for normal development of Myxococcus xanthus. J. Bacteriol. 177:7150-7154.
11. Hodgkin, J., and D. Kaiser. 1977. Cell-to-cell stimulation of motility in non-motile mutants of Myxococcus xanthus. Proc. Natl. Acad. Sci. USA 74:2938-2942.
12. Hopwood, D. A., and D. H. Sherman. 1990. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu. Rev. Genet. 24:37-66.
13. Innis, M. A., M. Tokunmaga, M. E. Williams, J. M. Loranger, S. Y. Chang, S. Chang, and H. C. Wu. 1984. Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene. Proc. Natl. Acad. Sci. USA 81: 3708-3712.
14. Isaki, L., M. Kawakami, R. Beers, R. Hom, and H. C. Wu. 1990. Cloning and nucleotide sequence of the Enterobacter aerogenes signal peptidase II (lsp) and flanking genes. J. Bacteriol. 172:469-472.
15. Isaki, L., R. Beers, and H. C. Wu. 1990. Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II (lsp) gene. J. Bacteriol. 172:6512-6517.
16. Kashefi, A., and P. L. Hartzell. 1995. Genetic suppression and phenotypic masking of a Myxococcus xanthus frzF-defect. Mol. Microbiol. 15:483-494.
17. Kroos, L., and D. Kaiser. 1984. Construction of Tn5lac, a transposon that fuses lacZ expression to exogenous promoters and its introduction into Myxococcus xanthus. Proc. Natl. Acad. Sci. USA 81:5816-5820.
18. Kroos, L., A. Kuspa, and D. Kaiser. 1986. A global analysis of developmentally regulated genes in Myxococcus xanthus. Dev. Biol. 117:252-266.
19. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydrophobic character of a protein. J. Mol. Biol. 157:105-132.
20. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
21. Miller, K. W., J. Bouvier, P. Stragier, and H. C. Wu. 1987. Identification of the genes in Escherichia coli iles-lsp operon. Analysis of multiple polycistronic mRNA made in vivo. J. Biol. Chem. 262:7391-7397.
22. Muñoa, F. J., K. Miller, R. Beers, M. Graham, and H. C. Wu. 1991 Membrane topology of Escherichia coli prolipoprotein signal peptidase (signal peptidase II). J. Biol. Chem. 266:17667-17672.
23. Norrander, J., T. Kempe, and J. Messing. 1983. Construction of improved M13 vector. Gene 26:101-106.
24. Paitan, Y., E. Orr, E. Z. Ron, and E. Rosenberg. 1999. A NusG-like transcription anti-terminator is involved in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus. FEMS Microbiol. Lett. 170:221-227.
25. Paitan, Y., E. Orr, E. Z. Ron, and E. Rosenberg. 1999. Cloning and characterization of a Myxococcus xanthus cytochrome P-450 hydroxylase required for biosynthesis of the polyketide antibiotic TA. Gene 228:147-153.
26. Paitan, Y., G. Alon, E. Orr, E. Z. Ron, and E. Rosenberg. 1999. The first gene in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus codes for a unique PKS module coupled to a peptide synthetase. J. Mol. Biol. 286:465-474.
27. Paitan, Y., N. Boulton, E. Z. Ron, E. Rosenberg, and E. Orr. 1998. Molecular analysis of the DNA gyrB gene from Myxococcus xanthus. Microbiology 144:1641-1647.
28. Pearson, W. R. 1990. Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 183:63-98.
29. Prágai, Z., H. Tjalsma, A. Bolhuis, J. van Dijl, G. Venema, and S. Bron. 1997. The signal peptidase II (lsp) gene of Bacillus subtilis. Microbiology 143:1327-1333.
30. Quillet, L., L. Bensmail, S. Barray, and J. Guespin-Michel. 1997. Cloning and sequencing of two genes, prtA and prtB, from Myxococcus xanthus, encoding PrtA and PrtB proteases, both of which are required for the protease activity. Gene 198:135-140.
31. Reichenbach, H. 1993. Biology of the myxobacteria: ecology and taxonomy, p. 13-62. In M. Dworkin and D. Kaiser (ed.), Myxobacteria II. American Society for Microbiology, Washington, D.C.
32. Rodriguez-Soto, J. P., and D. Kaiser. 1997. The tgl gene: social motility and stimulation in Myxococcus xanthus. J. Bacteriol. 179:4361-4371.
33. Rodriguez-Soto, J. F., and D. Kaiser. 1997. Identification and localization of Tgl protein, which is required for Myxococcus xanthus social motility. J. Bacteriol. 179:4372-4381.
34. Rosenberg, E., B. Vaks, and A. Zuckerberg. 1973. Bactericidal action of an antibiotic produced by Myxococcus xanthus. Antimicrob. Agents Chemother. 4:507-513.
35. Rosenberg, E, and M. Varon. 1984. Antibiotics and lytic enzymes, p. 109-125. In E. Rosenberg (ed.), Myxobacteria, development and cell interaction. Springer-Verlag, New York, N.Y.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
37. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
38. Sankaran, K., and H. C. Wu. 1994. Signal peptidase II-specific signal peptidase for bacterial lipoproteins, p. 17-29. In G. von Heijne (ed.), Signal peptidases. R. G. Landes Company, Austin, Tex.
39. Scotti, C., M. Piatti, A. Cuzzoni, P. Perani, A. Tognoni, G. Grandi, A. Galizzi, and A. M. Albertini. 1993. A Bacillus subtilis large ORF coding for a polypeptide highly similar to polyketide synthases. Gene 130:65-71.
40. Sedgwick, S. G., and B. A. Morgan. 1994. Locating, DNA sequencing and disrupting yeast genes using tagged Tn1000. Methods Mol. Genet. 3:131-140.
41. Shimkets, L. J. 1990. Social and developmental biology of myxobacteria. Microbiol. Rev. 54:473-501.
42. Sutcliffe, I. C., and R. R. B. Russell. 1995. Lipoproteins of gram-positive bacteria. J. Bacteriol. 177:1123-1128.
43. Tolchinsky, S., N. Fuchs, M. Varon, and E. Rosenberg. 1992. Use of Tn5lac to study expression of genes required for antibiotic TA production. Antimicrob. Agents Chemother. 36:2322-2327.
44. Varon, M., and E. Rosenberg. 1996. Transcriptional regulation of genes required for antibiotic TA synthesis in Myxococcus xanthus. FEMS Microbiol. Lett. 136:203-208.
45. Varon, M., N. Fuchs, M. Monosov, S. Tolchinsky, and E. Rosenberg. 1992. Mutation and mapping of genes involved in antibiotic TA production in Myxococcus xanthus. Antimicrob. Agents Chemother. 36:2316-2321.
46. Varon, M., Y. Paitan, and E. Rosenberg. 1997. Trans-acting regulation of antibiotic TA genes in Myxococcus xanthus. FEMS Microbiol. Lett. 155:141-146.
47. von Heijne, G. 1994. Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J. Mol. Biol. 225:487-494.
48. Witke, C., and F. Götz. 1995. Cloning and nucleotide sequence of the signal peptidase II from Staphylococcus carnosus. FEMS Microbiol. Lett. 126:233-240.
49. Wu, H. C., and M. Tokunaga. 1986. Biogenesis of lipoproteins in bacteria. Curr. Top. Microbiol. Immunol. 125:127-157.
50. Yamagata, H., C. Ippolito, M. Inukai, and M. Inouye. 1982. Temperature-sensitive processing of outer membrane lipoprotein in an Escherichia coli mutant. J. Bacteriol. 152:1163-1168.
51. Zafriri, D., E. Rosenberg, and D. Mirelman. 1981. Mode of action of Myxococcus xanthus antibiotic TA. Antimicrob. Agents Chemother. 19:349-351.
52. Zhao, X. J., and H. C. Wu. 1992. Nucleotide sequence of the Staphylococcus aureus signal peptidase II (lsp) gene. FEBS Lett. 299:80-84.