Analysis of Pseudomonas aeruginosa clinical isolates for possible variations within the virulence genes exotoxin A and exoenzyme S

Journal of Surgical Research

Volumn 82, Issue 1, 1999, Pages 95-105

  Rumbaugh, Kendra P ^a   Hamood, Abdul N ^a   Griswold, John A ^b  

^a TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^b TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER SCHOOL OF MEDICINE   (United States)

Author keywords

Clinical isolates; DNA hybridization; Exoenzyme S; Exotoxin A; P. aeruginosa

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; EXOENZYME S; EXOTOXIN A; PSEUDOMONAS EXOTOXIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CONTROLLED STUDY; DNA HYBRIDIZATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SOUTHERN BLOTTING; TRACHEA; TRACHEITIS; URINARY TRACT; URINARY TRACT INFECTION; WOUND INFECTION;

EID: 0032817057     PISSN: 00224804     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsre.1998.5523     Document Type: Article

References (49)

1. Bodey G., Bolivar R., Fainstein V., Jadeja L. Infections caused byPseudomonas aeruginosa. Rev. Infect. Dis. 5:1983;279.
2. Woods D. E., Iglewski B. H. Toxins ofPseudomonas aeruginosa: Rev. Infect. Dis. 5:1983;S715.
3. Cash H. A., Straus D. C., Bass J. A. Pseudomonas aeruginosa. Can. J. Microbiol. 29:1983;448.
4. Liu P. V. Extracellular toxins ofPseudomonas aeruginosa. J. Infect. Dis. 130:1974;S94.
5. Iglewski B. H., Kabat D. NAD-dependent inhibition of protein synthesis byPseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA. 2:1975;2284.
6. Iglewski B. H., Sadoff J. C., Bjorn M. J., Maxwell E. S. Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA. 75:1978;3211.
7. Gray G. L., Smith D. H., Baldridge J. S., Harkins R. N., Vasil M. L., Chen E. Y., Heynecker H. L. Cloning, nucleotide sequence, and expression inEscherichia coliPseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA. 81:1984;2645.
8. Coburn J., Dillon S. T., Iglewski B. H., Gill M. D. Exoenzyme S ofPseudomonas aeruginosa. Infect. Immun. 57:1989;996.
9. Frank D. W., Iglewski B. H. Cloning and sequence analysis of atransPseudomonas aeruginosa. J. Bacteriol. 173:1991;6460.
10. Nicas T. I., Iglewski B. H. Contribution of exoenzyme S to the virulence ofPseudomonas aeruginosa. Antibiot. Chemother. 36:1984;40.
11. Nicas T. I., Iglewski B. H. The contribution of exoproducts to virulence ofPseudomonas aeruginosa. Can. J. Microbiol. 31:1985;387.
12. Wick M. J., Frank D. W., Story D., Iglewski B. H. Structure, function, and regulation ofPseudomonas aeruginosa. Annu. Rev. Microbiol. 44:1990;335.
13. Liu P. V. Exotoxins ofPseudomonas aeruginosa: J. Infect. Dis. 128:1973;506.
14. Thompson M. R., Bjorn M. J., Sokol P. A., Lile J. D., Iglewski B. H. Exoenzyme S: An ADP-ribosyl transferase protein produced byPseudomonas aeruginosa. Novel ADP-Ribosylation of Regulatory Enzymes and Proteins. 1980;Elsevier/North-Holland, Amsterdam. p. 425-433.
15. Hamood A. H., Griswold J. A., Duhan C. M. Production of extracellular virulence factors byPseudomonas aeruginosa. J. Surg. Res. 61:1996;425.
16. Bartlet D. H., Silverman M. Nucleotide sequence of IS492, a novel insertion sequence causing variation in extracellular polysaccharide production in the marine bacteriumPseudomonas atlantica. J. Bacteriol. 171:1989;1763.
17. Nash J. H. E., Krishnapillai V. Identification of an insertion sequence in the chromosome ofPseudomonas aeruginosa. J. Bacteriol. 152:1982;514.
18. Pritchard A. E., Vasil M. L. Possible insertion sequences in a mosaic genome organization upstream of the exotoxin A gene inPseudomonas aeruginosa. J. Bacteriol. 172:1990;2020.
19. Woods D. E., Sokol P. A., Bryan L. E., Storey D. G., Mattingly S. J., Vogel H. J., Ceri H. In vivo regulation of virulence inPseudomonas aeruginosa. J. Infect. Dis. 163:1991;143.
20. Sokol P. A., Luan M., Storey D. G., Thirukkumaran P. Genetic rearrangement associated within vivoPseudomonas aeruginosa. J. Bacteriol. 176:1994;553.
21. Gomer J. S., Jarvis W. R., Emori T. G., Horan T. C., Hughes J. M. CDC definitions for nosocomial infections. Am. J. Infect. Control. 16:1988;128.
22. Liu P. V. The roles of various fractions ofPseudomonas aeruginosain vitroin vivo. J. Infect. Dis. 116:1966;481.
23. Bjorn M. J., Pavloviskis O. R., Thompson M. R., Iglewski B. H. Production of exoenzyme S duringPseudomonas aeruginosa. Infect. Immun. 24:1979;837.
24. Frank D. W., Nair G., Schweizer H. P. Construction and characterization of chromosomal insertional mutations of thePseudomonas aeruginosatrans. Infect. Immun. 62:1994;554.
25. Wick M. J., Iglewski B. H. Determination of the amino acid change responsible for the nontoxic, cross-reactive exotoxin A protein (CRM 66) ofPseudomonas aeruginosa. J. Bacteriol. 170:1988;5385.
26. Hamood A. N., Wick M. J., Iglewski B. H. Secretion of toxin A fromPseudomonas aeruginosaEscherichia coli. Infect. Immun. 58:1990;1133.
27. Miller J. H. Experiments in Molecular Genetics. 1972;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
28. Ausubel F., Brent R., Kingston R., Moor D., Seidman J., Smith J., Stauhle K. Current Protocols in Molecular Biology. 1988;Wiley-Interscience, New York.
29. Ohman D. E., Sadoff J. C., Iglewski B.H. Toxin A deficient mutants ofPseudomonas aeruginosa. Infect. Immun. 28:1980;899.
30. Nicas T. I., Iglewski B. H. Isolation and characterization of transposon induced mutants ofPseudomonas aeruginosa. Infect. Immun. 45:1984;470.
31. Kreig D. P., Bass J. A., Mattingly S. J. Aeration selects for mucoid phenotype ofPseudomonas aeruginosa. J. Clin. Microbiol. 24:1986;986.
32. White B. A. PCR Protocols: Current Methods and Applications. 1993;Humana Press, Totowa.
33. Goldberg J. B., Ohman D. E. Activation of an elastase precursor by thelasAPseudomonas aeruginosa. J. Bacteriol. 169:1987;4532.
34. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
35. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London). 227:1970;680.
36. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and application. Proc. Natl. Acad. Sci. USA. 76:1979;4350.
37. Vasil M. L., Chamberlain C., Grant C. C. Molecular studies ofPseudomonas. Infect. Immun. 52:1986;538.
38. Hamood A. N., Griswold J. A. DNA hybridization analysis of thePseudomonas aeruginosalasB. Can. J. Microbiol. 41:1995;910.
39. West S. E., Sample A., Runyen-Janecky L. ThevfrPseudomonas aeruginosa. J. Bacteriol. 176:1994;7532.
40. Hamood A. N., Colmer J. A., Ochsner U. A., Vasil M. L. Isolation and characterization of aPseudomonas aeruginosaptxR, Mol. Microbiol. 21:1996;97.
41. Cunliffe H. C., Merriman T. R., Lamont I. L. Cloning and characterization ofpvdS,Pseudomonas aeruginosa: J. Bacteriol. 177:1995;2744.
42. Prince R. W., Cox C. D., Vasil M. L. Coordinate regulation of siderophore and exotoxin A production: Molecular cloning and sequencing of thePseudomonas aeruginosa fur. J. Bacteriol. 175:1993;2589.
43. Colmer J. A., Hamood A. N. Characterization ofptxS;Pseudomonas aeruginosaptxR. Mol. Gen. Genet. 258:1998;250.
44. Stieritz D. D., Holder I. A. Experimental studies of the pathogenesis of infections due toPseudomonas aeruginosa: J. Infect. Dis. 131:1975;688.
45. Pavloviskis O. R., Iglewski B. H., Gordon F. B. Pseudomonas aeruginosa. J. Infect. Dis. 25:1972;631.
46. Pavloviskis O. R., Iglewski B. H., Pollack M. Mechanism of action ofPseudomonas aeruginosa. Infect. Immun. 19:1978;29.
47. Bjorn M., Vasil M., Sadoff J., Iglewski B. H. Incidence of exotoxin A production byPseudomonas. Infect. Immun. 16:1977;362.
48. Wick M. J., Frank D. W., Story D., Iglewski B. H. Identification ofregB,Pseudomonas aeruginosa. Mol. Microbiol. 4:1990;489.
49. Frank D. W. The exoenzyme S regulon ofPseudomonas aeruginosa. Mol. Microbiol. 26:1997;621.