Identification of the TIMP-2 binding site on the gelatinase A hemopexin C-domain by site-directed mutagenesis and the yeast two-hybrid system

Annals of the New York Academy of Sciences

Volumn 878, Issue , 1999, Pages 747-753

  Overall, Christopher M ^a   King, Angela E ^a   Bigg, Heather F ^a   McQuibban, Angus ^a   Atherstone, Juliet ^a   Sam, Douglas K ^a   Ong, Aldrich D ^a   Lau, Tim T Y ^a   Wallon, U Margaretha ^a   DeClerck, Yves A ^b   Tam, Eric ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GELATINASE A; HEMOPEXIN; TISSUE INHIBITOR OF METALLOPROTEINASE 2;

BINDING SITE; CONFERENCE PAPER; SITE DIRECTED MUTAGENESIS;

EID: 0032816546     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1999.tb07780.x     Document Type: Conference Paper

References (11)

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2. 2. Brown, P.D., A.T. Levy, I.M.K. Margulies, L.A. Liotta & W.G. StetlerStevenson, 1990. Independent expression and processing of Mr 72,000 type IV collagenase and interstitial collagenase in human tumorigenic cell lines. Cancer Res. 50: 6184-6191.
3. 3. Gilles, C., M. Polette, P. Birembaut & E.W. Thompson. 1997. Collagen type I-induced MT1-MMP expression and MMP-2 activation: implications in the metastatic progression of breast carcinoma. Lab. Invest. 76: 651-660.
4. 4. Overall, C.M., U.M. Wallon, B. Steffensen, Y. DeClerk, H. Tschesche & R. Abbey. 1999. Substrate and TIMP interactions with human gelatinase A recombinant COOH-terminal hemopexin-like and fibronectin type II-like domains: both the N- and C-domains of TIMP-2 bind the C-domain of gelatinase A. In Inhibitors of Matrix Metalloproteinases in Development and Disease. D. Edwards, S. Hawkes & R, Kokha, Eds. Gordon and Breach. Amsterdam. In press.
5. 5. Steffensen, B., H.F. Bigg & C.M. Overall. 1998. The involvement of the fibronectin Type II-like modules of human gelatinase A in cell surface localization and activation. J. Biol. Chem. 273: 20622-20628.
6. 2+ for fibronectin and heparin binding: binding properties of recombinant gelatinase A C-domain to extracellular matrix and basement membrane components. J. Biol. Chem. 272: 7473-7481.
7. 7. DeClerck, Y.A., T. Yean, H.S. Lu, J. Ting & K.E. Langley. 1991. Inhibition of autoproteolytic activation of the interstitial procollagenase by recombinant metalloproteinase inhibitor TIMP-2. J. Biol. Chem. 266: 3893-3899.
8. 8. Willenbrock, F., T. Crabbe, P.M. Slocombe, C.W. Sutton, A.J.P. Docherty, M.I. Cockett, M. O'Shea, K. Brocklehurst, I.R. Phillips & G. Murphy, 1993. The activity of the tissue inhibitors of metalloproteinases is regulated by C-terminal domain interactions: a kinetic analysis of the inhibition of gelatinase A. Biochemistry 32: 4330-4337.
9. 9. Bigg, H.F., Y.E. Shi, Y.E. Liu, B. Steffensen & C.M. Overall. 1997. Specific, high affinity binding of TIMP-4 to the COOH terminal hemopexin-like domain of human gelatinase A: TIMP-4 binds progelatinase A and the COOH terminal domain in a similar manner to TIMP-2. J. Biol. Chem. 272: 15496-15500.
10. 10. Li, J., P. Brick, M.C. O'Hare, T. Skarzynski, L.F. Lloyd, V.A. Curry, I.M. Clark, H.F. Bigg, B.L. Hazleman, T.E. Cawston & D.M. Blow. 1995. Structure of full-length porcine synovial collagenase reveals a C-terminal domain containing a calcium-linked, four bladed beta-propeller. Structure 3: 541-549.
11. 11. Overall, C.M., A.E. King, D.K. Sam, A.D. Ong, T.T.Y. Lau, U.M. Wallon, Y.A. DeClerck & J. Atherstone. 1999. Identification of the TIMP-2 binding site on the hemopexin carboxyl domain of human gelatinase A by site-directed mutagenesis. The hierarchical role in binding TIMP-2 of the unique cationic clusters of hemopexin modules III and IV. J. Biol. Chem. 274: 4421-4429.