Expression studies of CLN3 protein (battenin) in fusion with the green fluorescent protein in mammalian cells in vitro

Molecular Genetics and Metabolism

Volumn 66, Issue 4, 1999, Pages 277-282

  Golabek, Adam A ^a,b   Kaczmarski, Wojciech ^a   Kida, Elizabeth ^a,b   Kaczmarski, Aleksandra ^a   Michalewski, Martin P ^a   Wisniewski, Krystyna E ^a,c  

^a NEW YORK STATE INSTITUTE FOR BASIC RESEARCH IN DEVELOPMENTAL DISABILITIES   (United States)

^b MOSSAKOWSKI MEDICAL RESEARCH CENTRE   (Poland)

^c SUNY DOWNSTATE MEDICAL CENTER   (United States)

Author keywords

Batten disease; CLN3; GFP; In vitro expression

Indexed keywords

BATTENIN; CEROID; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; LIPOFUSCIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; CONFERENCE PAPER; GENETIC CODE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; NEURONAL CEROID LIPOFUSCINOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING;

EID: 0032811446     PISSN: 10967192     EISSN: None     Source Type: Journal    
DOI: 10.1006/mgme.1999.2836     Document Type: Conference Paper

References (15)

1. Sharp J D, Wheeler R B, Lake B D, Savukoski M, Järvelä I E, Peltonen L, Gardiner R M, Williams R E. Loci for classical and a variant late infantile neuronal ceroid lipofuscinosis map to chromosome 11p15 and 15q21-23. Hum Mol Genet. 6:1997;591-595.
2. Cell. 82:1995;949-957.
3. Munroe P B, Mitchison H M, O'Rawe A M, Anderson J W, Boustany R-M, Lerner T J, Taschner P EM, de Vos N, Breuning M H, Gardiner R M, Mole S E. Spectrum of mutations in the Batten disease gene, CLN3. Am J Hum Genet. 61:1997;310-316.
4. Janes R W, Munroe P B, Mitchison H M, Gardiner R M, Mole S E, Wallace B A. A model for Batten disease protein CLN3: Functional implications from homology and mutations. FEBS Lett. 399:1996;75-77.
5. Katz M L, Gao C-L, Prabhakaram M, Shibuya H, Liu P-C, Johnson G S. Immunochemical localization of the Batten disease (CLN3) protein in retina. Invest Ophthalmol Vis Sci. 38:1997;2375-2386.
6. Järvelä I, Sainio M, Rantamaki T, Olkkonen V M, Carpen O, Peltonen L, Jalanko A. Biosynthesis and intracellular targeting of the CLN3 protein defective in Batten disease. Hum Mol Genet. 7:1998;85-90.
7. Kaczmarski W, Kida E, Lach A, Rubenstein R, Zhong N, Wisniewski K E. Expression studies of CLN3 protein. Neuropediatrics. 28:1997;33-36.
8. Schägger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 166:1987;368-379.
9. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA. 76:1979;4350-4359.
10. Storrie B, Madden E A. Isolation of subcellular organelles. Methods in Enzymology. 1990;Academic Press, San Diego. p. 203-225.
11. Bordier C. Phase segregation of integral membrane proteins in Triton X-114 solution. J Biol Chem. 256:1981;1604-1607.
12. Town M, Jean G, Cherqui S, Attard M, Forestier L, Whitmore S A, Callen D F, Gribouval O, Broyer M, Bates G P, vant Hoff W, Antignac C. A novel gene encoding an integral membrane protein is mutated in nephropathic cystinosis. Nature Genet. 18:1998;319-324.
13. Granger B L, Green S A, Gabel C A, Howe C L, Mellman I, Helenius A. Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells. J Biol Chem. 265:1990;12036-12043.
14. Peters C, von Figura K. Biogenesis of lysosomal membranes. FEBS Lett. 346:1994;108-114.
15. Hunziker W, Geuze H J. Intracellular trafficking of lysosomal membrane protein. BioEssays. 18:1996;379-389.