Expression studies of CLN3 protein

Neuropediatrics

Volumn 28, Issue 1, 1997, Pages 33-36

  Kaczmarski, W ^a   Kida, E ^a   Lach, A ^a   Rubenstein, R ^a   Zhong, N ^a   Wisniewski, K E ^a  

^a NEW YORK STATE INSTITUTE FOR BASIC RESEARCH IN DEVELOPMENTAL DISABILITIES   (United States)

Author keywords

Batten disease; CLN3; Expression systems; Reticulocytes

Indexed keywords

GENE PRODUCT; HYBRID PROTEIN; PALMITOYL PROTEIN THIOESTERASE; THIOL ESTER HYDROLASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; IMMUNOPRECIPITATION; MOLECULAR WEIGHT; NEURONAL CEROID LIPOFUSCINOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS INHIBITION; RABBIT; RETICULOCYTE;

EID: 0030788955     PISSN: 0174304X     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2007-973663     Document Type: Article

References (15)

1. Baker, T. A., A. D Grossman, C. A. Gross: A gene regulating the heat shock response in Escherichia coli also affects proteolysis Proc. Natl. Acad Sci USA 81 (1984) 6779-6783
2. Chang, J.-Y.: Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate. Eur. J. Biochem. 151 (1985) 217-224
3. Ferreira, G. C., P. L. Pedersen. Overexpression of higher eucaryotic membrane proteins in bacteria. J Biol. Chem. 267 (1992) 5460-5466
4. Fangioni, J. V., B. G. Neel: Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal. Biochem 210 (1993) 179-187
5. Gutman, G. A., G. W. Hartfield: Nonrandom utilization of codon pairs in Escherichia coll. Proc. Natl. Acad. Sci. USA 86 (1989) 3699-3703
6. Khosravi-Far, R., C. J. Der: Prenylation analysis of bacterially expressed and insect cell-expressed Ras and Ras-related proteins. Methods in Enzymol. 255 (1995) 46-60
7. Krieg, P., D. Melton: Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic. Acids Res. 12 (1984) 7057-7070
8. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
9. Nagai, K., M. F. Perutz, C. Poyart: Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli. Proc. Natl. Acad. Sci USA 82 (1985) 7252-7255
10. Pelham, H R. B., J. R. Jackson: An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67 (1976) 247-256
11. Pratt, R. D., G C. Ferreira, P. L. Pedersen: Mitochondrial protein transport. J Biol. Chem. 266 (1991) 1276-1280
12. Smith, D. B., K. S. Johnson: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67 (1988) 31-40
13. Studier, F. W., B. A. Moffatt: Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol 189 (1986) 113-130
14. The International Batten Disease Consortium: Isolation of a novel gene underlying Batten disease, CLN3. Cell 82 (1995) 949-957
15. Towbin, H., T. Staehelin, J. Gordon: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci USA 76 (1979) 4350-4354