Quantitative autoradiography with short-lived positron emission tomography tracers: A study on muscarinic acetylcholine receptors with N- [11C]methyl-4-piperidylbenzilate

Journal of Pharmacology and Experimental Therapeutics

Volumn 290, Issue 2, 1999, Pages 917-922

  Sihver, Sven ^a,b   Sihver, Wiebke ^a,b   Bergström, Mats ^a,b,c   Höglund, A Urban ^a   Sjöberg, Pernilla ^c   Långström, Bengt ^b,c   Watanabe, Yasuyoshi ^a,b,d  

^a UPPSALA UNIVERSITY   (Sweden)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c UPPSALA UNIVERSITY HOSPITAL   (Sweden)

^d OSAKA BIOSCIENCE INSTITUTE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

1 METHYL 4 PIPERIDYL BENZILATE; CARBON 11; MUSCARINIC M4 RECEPTOR; MUSCARINIC RECEPTOR; TRACER;

ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; BINDING AFFINITY; BINDING SITE; BRAIN SLICE; BRAIN TISSUE; IMAGING; LIGAND BINDING; MALE; NONHUMAN; POSITRON EMISSION TOMOGRAPHY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; RAT; RECEPTOR BINDING; THICKNESS;

ANIMALS; AUTORADIOGRAPHY; BENZILATES; CEREBRAL CORTEX; HALF-LIFE; IMAGE PROCESSING, COMPUTER-ASSISTED; MALE; NEOSTRIATUM; PIPERIDINES; RADIOPHARMACEUTICALS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, MUSCARINIC; SPINAL CORD; TOMOGRAPHY, EMISSION-COMPUTED;

EID: 0032808422     PISSN: 00223565     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Biegon A, Duvdevani R, Greenberger V and Segal M (1988) Aging and brain cholinergic muscarinic receptors: An autoradiographic study in the rat. J Neurochem 51:1381-1385.
2. Cortes R and Palacios JM (1986) Muscarinic cholinergic receptor subtypes in the rat brain. I. Quantitative autoradiographic studies. Brain Res 362:227-238.
3. 3H-labeled compounds in rat tumours. Int J Radiat Appl Instrum Part B 15:577-585.
4. 11C with double-tracer applications. Int J Appl Radiat Isot 35:129-134.
5. 11C]raclopride in man. Synapse 20:200-208.
6. Flynn DD, Reever CM and Ferrari-DiLeo G (1997) Pharmacological strategies to selectively label and localize muscarinic receptor subtypes. Drug Dev Res 40:104-116.
7. 3H]-Quinuclidinyl benzilate binding to muscarinic receptors in rat brain: Comparison of results from intact brain slices and homogenates. Br J Pharmacol 66:451-456.
8. 3H]raclopride to rat and human brain membranes. J Neurochem 55:2048-2057.
9. Höglund AU and Baghdoyan HA (1997) M2, M3 and M4 but not M1, muscarinic receptor subtypes are present in rat spinal cord. J Pharmacol Exp Ther 281:470-477.
10. Hulme EC and Birdsall NJM (1993) Strategy and tactics in receptor-binding studies, in Receptor-Ligand Interactions. A Practical Approach (Hulme EC ed) pp 63-176, Oxford University Press, Oxford.
11. Jerusalinsky D and Harvey AL (1994) Toxins from mamba venoms: Small proteins with selectivities for different subtypes of muscarinic acetylcholine receptors. Trends Pharmacol Sci 15:424-430.
12. Jolkkonen M, van Giersbergen PLM, Hellman U, Wernstedt C and Karlsson E (1994) A toxin from the green mamba Dendroaspis angusticeps: Amino acid sequence and selectivity for muscarinic m4 receptors. FEBS Lett 352:91-94.
13. Kloog Y, Egozi Y and Sokolovsky M (1979) Characterization of muscarinic acetylcholine receptors from mouse brain: Evidence for regional heterogeneity and isomerization. Mol Pharmacol 15:545-558.
14. Kuhar MJ (1985) Receptor localization with the microscope, in Neurotransmitter Receptor Binding (Yamamura HI, Enna SJ and Kuhar MJ eds) pp 153-176, Raven Press, New York.
15. 11C)methionine. J Nucl Med 28:1037-1040.
16. Matsumura K, Bergström M, Onoe H, Takechi H, Westerberg G, Antoni G, Bjurling P, Jacobson GB, Långström B and Watanabe Y (1995) In vitro positron emission tomography (PET): Use of positron emission tracers in functional imaging in living brain slices. Neurosci Res 22:219-229.
17. Mulholland GK, Jewett DW, Otto CA, Kilbourn MR, Sherman PS and Kuhl DE (1988) Synthesis and regional brain distribution of [C-11]N-methyl-4-piperidyl benzilate ([C-11]NMPB) in the rat. J Nucl Med 29:768.
18. 11C]Ro15-1788. Neurosci Res 25:145-154.
19. Murata T, Matsumura K, Sihver S, Onoe H, Bergström M, Sihver W, Yonekura Y, Långström B and Watanabe Y (1998) Triazolam-induced modulation of muscarinic acetylcholine receptor in living brain slices as revealed by a new positron-based imaging technique. J Neural Transm 105:1117-1127.
20. 11C]Ro 15-1788. Nucl Med Biol 24:723-731.
21. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ and Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76-85.
22. Tang C, Biemond I and Lamers CBHW (1995) Localisation and quantification of cholecystocinin receptors in rat brain with storage phosphor autoradiography. BioTechniques 18:886-889.
23. 3H-labeled ligands visualized by imaging plates. NeuroReport 3:961-964.