TRI12, a trichothecene efflux pump from Fusarium sporotrichioides: Gene isolation and expression in yeast

Molecular and General Genetics

Volumn 261, Issue 6, 1999, Pages 977-984

  Alexander, N J ^a   McCormick, S P ^a   Hohn, T M ^b  

^a NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Efflux pump; Fusarium sporotrichioides; Major facilitator superfamily; TRI12; Trichothecenes

Indexed keywords

ACYLTRANSFERASE; POLYPEPTIDE; TRICHOTHECENE;

ARTICLE; CELL MEMBRANE TRANSPORT; CULTURE MEDIUM; EFFUSION; FUNGAL GENETICS; FUSARIUM; GENE CLUSTER; GENE ISOLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PUMP; SEQUENCE HOMOLOGY; TOXIN SYNTHESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CLONING, MOLECULAR; DNA, FUNGAL; FUNGAL PROTEINS; FUSARIUM; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC; TRICHOTHECENES;

FUNGI; FUSARIUM SPOROTRICHIOIDES; MYXOGASTRIA;

EID: 0032804706     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380051046     Document Type: Article

References (34)

1. th International Congress Molecular Plant-Microbe Interactions, Knoxville, Tenn. (Abstract B-3)
2. Alexander N, Hohn TM, McCormick SP (1998) The TRI11 gene of Fusarium sporotrichioides encodes a cytochrome P450 mono-oxygenase required for C-15 hydroxylation in trichothecene biosynthesis. Appl Environ Microbiol 64:221-225
3. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410
4. Balzi E, Wang M, Leterme S, Dyck LV, Goffeau A (1994) PDR5, a novel yeast multidrug resistance-conferring transporter controlled by the transcription regulator PDR1. J Biol Chem 269:2206-2214
5. th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington DC, p 370
6. Cundliffe E (1989) How antibiotic-producing organisms avoid suicide. Annu Rev Microbiol 43:207-233
7. Desjardins AE, Hohn TM, McCormick SP (1993) Trichothecene biosynthesis in Fusarium species: chemistry, genetics, and significance. Microbiol Rev 57:595-604
8. Desjardins AE, Proctor RH, Bai G, McCormick SP, Shaner G, Buechley G, Hohn TM (1996) Reduced virulence of trichothecene-nonproducing mutants of Gibberella zeae in wheat field tests. Mol Plant-Microbe Interact 9:775-781
9. Ehrenhofer-Murray AE, Wurgler FE, Sengstag C (1994) The Saccharomyces cerevisiae SGE1 gene product: a novel drug-resistance protein within the major facilitator superfamily. Mol Gen Genet 244:287-294
10. Fried HM, Warner JR (1981) Cloning of yeast gene for trichodermin resistance and ribosomal protein L3. Proc Natl Acad Sci USA 78:238-242
11. Gietz RD, Sugino A (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534
12. Gietz RD, St. Jean A, Woods RA, Schiestl RH (1992) Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res 20:1425
13. Goffeau A, Park J, Paulsen IT, Jonniaux J-L, Dinh T, Mordant P, Saier MH (1997) Multidrug-resistant transport proteins in yeast: complete inventory and phylogenetic characterization of yeast open reading frames within the major facilitator super-family. Yeast 13:43-54
14. Grgurina I, Gross DC, Iacobellis NS, Lavermicocca P, Takemoto JY, Benincasa M (1996) Phytotoxin production by Pseudomonas syringae pv syringae: syringopeptin production by syr mutants defective in biosynthesis or secretion of syringomycin. FEMS Microbiol Lett 138:35-39
15. Hofmann K, Stoffel W (1993) TMbase - a database of membrane- spanning protein segments. Biol Chem Hoppe-Seyler 347:166
16. Hohn TM, McCormick SP, Desjardins AE (1993) Evidence for a gene cluster involving trichothecene pathway biosynthetic genes in Fusarium sporotrichioides. Curr Genet 24:291-295
17. Hohn TM, Desjardins AE, McCormick SP, Proctor RH (1995) Biosynthesis of trichothecenes, genetic and molecular aspects: issues involving toxic microorganisms. In: Eklund M, Richard JL, Katsutoshi M (eds) Molecular approaches to food safety. Alaken Inc., Fort Collins, Colo., pp 239-247
18. Hohn TM, McCormick SP, Alexander NJ, Desjardins AE, Proctor RH (1998) Function and biosynthesis of trichothecenes produced by Fusarium species. In: Kohmoto K, Yoder OC (eds) Molecular genetics of host-specific toxins in plant disease. Kluwer, Dordrecht, pp 17-24
19. Kimura M, Kaneko I, Komiyama M, Takatsuki A, Koshino H, Yoneyama K, Yamaguchi I (1998a) Trichothecene 3-O-acetyltransferase protects both the producing organism and transformed yeast from related mycotoxins. Cloning and characterization of Tri101. J Biol Chem 273:1654-1661
20. Kimura M, Shingu Y, Yoneyama M, Yamaguchi I (1998b) Features of Tri101, the trichothecene 3-O-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum. Biosci Biotechnol Biochem 62:1033-1036
21. Kimura M, Matsumoto G, Shingu Y, Yoneyama K, Yamaguchi I (1998c) The mystery of the trichothecene 3-O-acetyltransferase gene. FEBS Lett 435:163-168
22. Marger MD, Saier MH (1993) A major superfamily of trans-membrane facilitators that catalyse uniport, symport and antiport. Trends Biochem Sci 18:13-20
23. McCormick SP, Hohn TM (1997) Accumulation of trichothecenes in liquid cultures of a Fusarium sporotrichioides mutant lacking a functional trichothecene C-15 hydroxylase. Appl Environ Microbiol 63:1685-1688
24. McCormick SP, Hohn TM, Desjardins AE (1996) Isolation and characterization of Tri3, a gene encoding 15-O-acetyltransferase from Fusarium sporotrichioides. Appl Environ Microbiol 62:353-359
25. McLaughlin CS, Vaughn MH, Campbell JM, Wei CM, Stafford ME, Hansin BS (1977) Inhibition of protein synthesis by trichothecenes. In: Rodricks HV, Hesseltine CW, Mehlman MA (eds) Mycotoxins in human and animal health. Pathotoxin Publishers, Park Forest, Ill., pp 263-273
26. Meyers S, Schauer W, Balzi E, Wagner M, Goffeau A, Golin J (1992) Interaction of the pleiotrophic drug resistance genes PDR1 and PDR5. Curr Genet 21:431-436
27. Paulsen IT, Brown MH, Skurray RA (1996) Proton-dependent multidrug efflux systems. Microbiol Rev 60:575-608
28. Pitkin JW, Panaccione DG, Walton JD (1996) A putative cyclic peptide efflux pump encoded by the TOXA gene of the plant-pathogenic fungus Cochliobolus carbonum. Microbiology 142:1557-1565
29. Proctor RH, Hohn TM, McCormick SP (1995a) Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Mol Plant-Microbe Interact 8:593-601
30. Proctor RH, Hohn TM, McCormick SP, Desjardins AE (1995b) Tri6 encodes an unusual zinc finger protein involved in the regulation of trichothecene biosynthesis in Fusarium sporotrichioides. Appl Environ Microbiol 61:1923-1930
31. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
32. Turgeon BG, Garber RC, Yoder OC (1987) Development of a fungal transformation system based on selection of sequences with promoter activity. Mol Cell Biol 7:3297-3305
33. Ueno Y (1980) Trichothecene mycotoxins: mycology, chemistry, and toxicology. Adv Nutr Sci 3:301-351
34. Ueno Y, Sawano M, Ishii K (1975) Production of trichothecene mycotoxins by Fusarium species in liquid shake culture. Appl Microbiol 30:4-9