Cloning, sequencing, expression and allelic sequence diversity of ERG3 (C-5 sterol desaturase gene) in Candida albicans

Gene

Volumn 236, Issue 1, 1999, Pages 43-51

  Miyazaki, Yoshitsugu ^a   Geber, Antonia ^b   Miyazaki, Haruko ^a   Falconer, Derek ^c   Parkinson, Tanya ^c   Hitchcock, Christopher ^c   Grimberg, Brian ^a   Nyswaner, Katherine ^a   Bennett, John E ^a  

^a Sect Clin and Exp Neuropsychol   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^c PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

Author keywords

Darlington; Ergosterol; GAL1; Heterozygosity; Yeast

Indexed keywords

ALLELE; ARTICLE; CANDIDA ALBICANS; CELL TRANSFORMATION; ENZYME ACTIVITY; GAS CHROMATOGRAPHY; GENE EXPRESSION; MASS SPECTROMETRY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STEROL SYNTHESIS;

AMINO ACID SEQUENCE; AZOLES; BLOTTING, SOUTHERN; CANDIDA ALBICANS; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, FUNGAL; GENOTYPE; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PHENOTYPE; SEQUENCE HOMOLOGY, AMINO ACID; STEROLS; TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC;

EID: 0032783722     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00263-2     Document Type: Article

References (32)

1. Arthington B.A., Bennett L.G., Skatrud P.L., Guynn C.J., Barbuch R.J., Ulbright C.E., Bard M. Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase. Gene. 102:1991;39-44.
2. Arthington-Skaggs B.A., Crowell D.N., Yang H., Sturley S.L., Bard M. Positive and negative regulation of a sterol biosynthesis gene (ERG3) in the post-squalene portion of the yeast ergosterol pathway. FEBS Lett. 392:1996;161-165.
3. Birky C.W. Jr. Heterozygosity, heteromorphism and phylogenetic trees in an asexual eukaryote. Genetics. 144:1996;427-437.
4. Daum G., Lees N.D., Martin M., Dickson R. Biochemistry, cell biology and molecular biology of lipids of Saccharomyces cerevisiae. Yeast. 14:1998;1471-1510.
5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:1984;387-395.
6. Fujimura H., Sakuma Y. Simplified isolation of chromosomal and plasmid DNA from yeast. BioTechniques. 14:1993;538-539.
7. Gachotte D., Hasselstein T., Bard M., Lacroute F., Benveniste P. Isolation and characterization of an Arabidopsis thaliana cDNA encoding a Δ7-sterol-C-5-desaturase by functional complementation of a defective yeast mutant. Plant J. 9:1996;391-398.
8. Geber A., Hitchcock C.A., Swartz J.E., Pullen F.S., Marsden K.E., Kwon-Chung K.J., Bennett J.E. Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition and antifungal susceptibility. Antimicrob. Agents Chemother. 39:1995;2708-2717.
9. Gietz R.D., Woods R.A. High efficiency transformation in yeast. Johnston J.W. Molecular Genetics of Yeast: Practical Approaches. 1994;121-134 Oxford University Press.
10. Gorman J.A., Gorman J.W., Koltin Y. Direct selection of galactokinase-negative mutants of Candida albicans using 2- deoxy-galactose. Curr. Genet. 21:1992;203-206.
11. Goshorn A.K., Grindle S.M., Scherer S. Gene isolation by complementation in Candida albicans and applications to physical and genetic mapping. Infect. Immun. 60:1992;876-884.
12. Hemmi K.C., Julmanop C., Hirata D., Tsuchiya E., Takemoto J.Y., Miyakawa T. The physiological roles of membrane ergosterol as revealed by the phenotypes of syr1/erg3 null mutants of Saccharomyces cerevisiae. Biosci. Biotech. Biochem. 59:1995;482-486.
13. Howell S.A., Mallet A.I., Noble W.C. A comparison of the sterol content of multiple isolates of the Candida albicans Darlington strain with other clinically azole-sensitive and -resistant strains. J. Appl. Bacteriol. 69:1990;692-696.
14. Jute T.H., Osawa S. CUG codons in Candida spp. J. Mol. Evolution. 42:1996;321-322.
15. Kelly R., Miller S.M., Kurtz M.B., Kirsch D.R. Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants. Mol. Cell. Biol. 7:1987;199-208.
16. Kirsch D.R., Kelly R., Kurtz M.B. The Genetics of Candida. 1990;CRC Press, Boca Raton, FL.
17. Kirsch D.R., Lai M.H., O'Sullivan J. Isolation of the gene for cytochrome P450L1A1 (lanosterol 14alpha-demethylase) from Candida albicans. Gene. 68:1988;229-237.
18. Losberger C., Ernst J.F. Sequence of the Candida albicans gene encoding actin. Nucleic Acids Res. 17:1989;9488.
19. Miyasaki S.H., White T.C., Agabian N. A fourth secreted aspartyl proteinase gene (SAP4) and a CARE2 repetitive element are located upstream of the SAP1 gene in Candida albicans. J. Bacteriol. 176:1994;1702-1710.
20. Pujol C., Reynes J., Renaud F., Raymond M., Tibayrenc M., Ayala F.J., Janbon F., Mallie M., Bastide J.M. The yeast Candida albicans has a clonal mode of reproduction in a population of infected human immunodeficiency virus-positive patients. Proc. Natl. Acad. Sci. USA. 90:1993;9456-9459.
21. Rustchenko E.P., Howard D.H., Sherman F. Chromosomal alterations of Candida albicans are associated with the gain and loss of assimilating functions. J. Bacteriol. 176:1994;3231-3241.
22. Rustchenko-Bulgac E.P., Sherman F., Hicks J.B. Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J. Bacteriol. 172:1990;1276-1283.
23. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual. 2nd edition :1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Sikorski R.E., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:1989;19-27.
25. Smith S.J., Crowley J.H., Parks L.W. Transcriptional regulation by ergosterol in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 16:1996;5427-5432.
26. Taylor F.R., Rodriguez R.J., Parks L.W. Requirement for a second sterol biosynthetic mutation for viability of a sterol C-14 demethylation defect in Saccharomyces cerevisiae. J. Bacteriol. 155:1983;64-68.
27. Varma A., Edman J.C., Kwon-Chung J.K. Molecular and genetic analysis of URA5 transformants of Cryptococcus neoformans. Infect. Immun. 60:1992;1101-1108.
28. Warnock D.W., Johnson E.M., Richardson M.D., Vickers C.F.H. Modified response to ketoconazole of Candida albicans from a treatment failure. Lancet. i:1983;642-643.
29. Watson P.E., Rose M.E., Ellis S.W., England H., Kelly S.L. Defective sterol C5-6 desaturation and azole resistance: a new hypothesis for the mode of action of azole antifungals. Biochem. Biophys. Res. Commun. 164:1989;1160-1175.
30. Wong B., Leeson S., Grindle S., Magee B., Brooks E., Magee P.T. D-Arabitol metabolism in Candida albicans: construction and analysis of mutants lacking D-arabitol dehydrogenase. J. Bacteriol. 177:1995;2971-2976.
31. White T.C. The presence of an R467K amino acid substitution and loss of allelic variation correlate with an azole-resistant lanosterol 14-α demethylase in Candida albicans. Antimicrob. Agents Chemother. 41:1997;1488-1494.
32. Zaret K.S., Sherman F. DNA sequence required for efficient transcription termination in yeast. Cell. 28:1982;563-573.