Phosphorylation by DNAPK inhibits the DNA-binding function of p53/T antigen complex in vitro

Anticancer Research

Volumn 19, Issue 3 A, 1999, Pages 2079-2083

  Sheppard, Hilary M ^a   Liu, Xuan ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

DNAPK; p53; T antigen

Indexed keywords

DNA DEPENDENT PROTEIN KINASE; DOUBLE STRANDED DNA; PROTEIN MDM2; PROTEIN P53;

ARTICLE; CONFORMATIONAL TRANSITION; DNA BINDING; DNA STRAND BREAKAGE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION;

ANIMALS; ANTIGENS, POLYOMAVIRUS TRANSFORMING; CELL TRANSFORMATION, VIRAL; DNA; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; MACROMOLECULAR SUBSTANCES; MICE; MICE, SCID; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN-SERINE-THREONINE KINASES; STRUCTURE-ACTIVITY RELATIONSHIP; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0032781658     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Ko LJ, and Prives C: p53: Puzzle and paradigm. Genes De7 10: 1054 1072, 1996.
2. Levine AJ: p53, the cellular gatekeeper for the growth and division. Cell 88: 323-331, 1997.
3. Bargonetti J, Reynisdottir I, Friedman P, and Prives C: Site-specific binding of wild-type p53 J to cellular DNA is inhibited by SV40 T antigen and mutant p53. Genes Dev6: 1886-1898, 1992.
4. Sheppard HM, Corneillie SI, Espiritu C, Gatti A and Liu X: New insights into the mechanism of inhibition of p53 by SV40 large T antigen. Mol Cell Biol. in press. 1999.
5. Nelson WG, and Kastan MB: DNA strand breaks: the DNA template alterations that trigger p53-dependent DNA damage response pathways. Mol Cell Biol 14: 1815-1823, 1994.
6. Milczareck GJ, Martinez J, and Bowden GT: p53 phosphorylation: biochemical and functional consequences. Life Sci 60: 1-11, 1997.
7. Lees-Miller SP, Kazuysasu S, Ullrich SJ, Appella E, and Anderson CW: Human DNA- activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of p53. Mol Cell Biol 12: 5041-5049, 1992.
8. Gottileb TM and Jackson SP: The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72: 131-142, 1993.
9. Lees-Miller SP, Godbout R, Chan DW, Weinfeld M, Day III RS, Barron GM, and Allalunis- Turner J: Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line. Science 267: 1183-1185, 1995.
10. Kirchgessner CU, Patil CK, Evans JW, Cuomo CA, Fried LM, Carter T, Oettinger MA, and Brown JM: DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267: 1178-1183, 1995.
11. Peterson SR, Kurimasa A, Oshimura M, Dynan WS, Bradbury EM, Chen DJ: Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand-break-repair mutant mammalian cells. PNAS USA 92: 3171-3174, 1995.
12. Fulop GM and Phillips RA: The seid mutation in mice causes a general defect in DNA repair. Nature 347: 479-482, 1990.
13. Biedermann KA, Sun JR, Giaccia AJ, Tosto LM, Brown JM: seid mutation in mice confers hypersensitivity to ionizing radiation and a deficiency in DNA double-strand break repair. PNAS USA 88: 1394-1397, 1991.
14. Shieh SY, Ikeda M, Taya Y, Prives C: DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2. Cell 91: 325-334, 1997.
15. Mayo LD, Turchi JJ, Berberich SJ: Mdm-2 phosphorylation by DNA-dependent protein kinase prevents interaction with p53. Cancer Res. 57: 5013-5016, 1997.
16. Prives C: Signalling to p53:Breaking the MDM2-p53 circuit. Cell 95: 5-8, 1998.
17. Dignam JD, Lebovitz RM, and Roeder RG: Accurate transcript initiation by RNA Pol II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 11: 1475-1489, 1983.
18. Liu X, and Berk AJ: Reversal of in vitro p53 squelching by both TFIIB and TFIID. Mol Cell Biol 15: 6474-6478, 1995.
19. Anderson CW, and Lees-Miller SP: The nuclear serine/threonine protein kinase DNA-PK. Crit Revs Eukar Gene Exp 2: 283-314, 1992.
20. Jayaraman L, Murthy KGK, Zhu C, Curran T, Xanthoudakis S, and Prives C: Identification of redox/repair protein Ref-1 as a potent activator of p53. Genes Dev 11: 558-570, 1997.