Isolation of rifampin-resistant mutants of Listeria monocytogenes and their characterization by rpoB gene sequencing, temperature sensitivity for growth, and interaction with an epithelial cell line

Journal of Clinical Microbiology

Volumn 37, Issue 9, 1999, Pages 2913-2919

  Morse, Robert ^a   O'Hanlon, Karen ^a   Virji, Mumtaz ^b   Collins, Matthew D ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE; RIFAMPICIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIUM ISOLATION; GENE SEQUENCE; GROWTH REGULATION; IMMUNOFLUORESCENCE; LISTERIA MONOCYTOGENES; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL;

LISTERIA MONOCYTOGENES;

EID: 0032779973     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.9.2913-2919.1999     Document Type: Article

References (32)

1. Aubry-Damon, H., C.-J. Soussy, and P. Courvalin. 1998. Characterization of mutations in the rpoB gene that confer rifampin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 42:2590-2594.
2. B from Listeria monocytogenes and its role in osmotolerance. J. Bacteriol. 180:4547-4554.
3. Bhatnager, N., E. Getachew, S. Straley, J. Williams, M. Meltzer, and A. Fortier. 1994. Reduced virulence of rifampin-resistant mutants of Francisella tularensis. J. Infect. Dis. 170:841-847,
4. Bjorkman, J., D. Hughes, and D. L. Andersson. 1998. Virulence of antibiotic-resistant Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 95:3939-3953.
5. Boisivon, A., C. Guiomar, and C. Carbon. 1990. In vitro bactericidal activity of amoxicillin, gentamicin, rifampin, ciprofloxacin and trimethoprim-sulfa-methoxazole alone or in combination against Listeria monocytogenes. Eur. J. Clin. Microbiol. Infect. Dis. 9:206-209.
6. S-dependent overexpression of ftsZ in an Escherichia coli K12 rpoB mutant that is resistant to the division inhibitors DicB and DicF RNA. Mol. Gen. Genet. 248:190-194.
7. Carter, P. E., F. J. R. Abadi, D. E. Yakuba, and T. H. Pennington. 1994. Molecular characterization of rifampin-resistant Neisseria meningitidis. Antimicrob. Agents Chemother. 38:1256-1261.
8. Chakraborty, T., M. Leimeister-Wachter, E. Domann, M. Hartl, W. Goebel, T. Nichterlein, and S. Notermans. 1992. Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene. J. Bacteriol. 174:568-574.
9. Cossart, P., and M. Lecuit. 1998. Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: bacterial factors, cellular ligands and signaling. EMBO J. 17:3797-3806.
10. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
11. Gaillard, J. L., P. Berche, J. Mounier, S. Richard, and P. Sansonetti. 1987. Penetration of Listeria monocytogenes into the host: a crucial step of the infectious process. Ann. Inst. Pasteur Microbiol. 138:259-264.
12. Ireton, K., and P. Cossart. 1997. Host-pathogen interactions during entry and actin-based movement of Listeria monocytogenes. Annu. Rev. Genet. 31:113-138.
13. Jin, D. J., and C. A. Gross. 1989. Characterization of the pleiotropic phenotypes of rifampin-resistant rpoB mutants of Escherichia coli. J. Bacteriol. 171:5229-5231.
14. Kaufmann, S. H. E. 1991. Heat-shock proteins and pathogenesis of bacterial infections. Springer Semin. Immunopathol. 13:25-36.
15. Lawson, P. A., S. E. Gharbia, H. N. Shah, and D. R. Clark. 1989. Recognition of Fusobacterium nucleatum subgroups FN-1, FN-2 and FN-3 by ribosomal RNA gene restriction patterns. FEMS Microbiol. Lett. 65:41-45.
16. Marron, L., N. Emerson, C. G. M. Gahan, and C. Hill. 1997. A mutant of Listeria monocytogenes LO28 unable to induce an acid tolerance response displays diminished virulence in a murine model. Appl. Environ. Microbiol. 63:4945-4947.
17. McDermot-Lancaster, D., and G. R. F. Hilson. 1987. Rifampin-resistant strains of Mycobacterium leprae may have reduced virulence. J. Med. Microbiol. 24:13-15.
18. Mekalanos, J. J. 1992. Environmental signals controlling expression of virulence determinants in bacteria. J. Bacteriol. 174:1-7.
19. Moorman, D. R., and G. L. Mandell. 1981. Characterization of rifampin-resistant variants obtained from clinical isolates of Staphylococcus aureus. Antimicrob. Agents Chemother. 20:709-713.
20. O'Driscoll, B., C. G. M. Gahan, and C. Hill. 1996. Adaptive acid tolerance response in Listeria monocytogenes: isolation of an acid-tolerant mutant which demonstrates increased virulence. Appl. Environ. Microbiol. 62:1693-1698.
21. 7O-conjugated chemical protease. Proc. Natl. Acad. Sci. USA 95:6021-6026.
22. Portillo-Gomez, L., J. Nair, D. A. Rouse, and S. L. Morris. 1995. The absence of genetic markers for streptomycin and rifampin resistance in Mycobacterium avium complex strains. J. Antimicrob. Chemother. 36:1049-1053.
23. Portnoy, D. A., T. Chakraborty, W. Goebel, and P. Cossart. 1992. Molecular determinants of Listeria monocytogenes pathogenesis. Infect. Immun. 60: 1263-1267.
24. Ripio, M.-T., G. Dominguez-Bernal, M. Lara, M. Súarez, and J.-A. Vázquez-Boland. 1997. A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes. J. Bacteriol. 179:1533-1540.
25. 38 (the rpoS gene product). Nucleic Acids Res. 23:827-834.
26. Tribuddharat, C., and M. Fennewald. 1999. Integron-mediated rifampin resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 43: 960-962.
27. Van Langendonck, N., E. Bottreau, S. Bailly, M. Tabouret, J. Marly, P. Pardon, and P. Velge. 1998. Tissue culture assays using Caco-2 cell line differentiate virulent from non-virulent Listeria monocytogenes strains. J. Appl. Microbiol. 85:337-346.
28. Virji, M., S. M. Watt, S. Barker, K. Makepeace, and R. Doyonnas. 1996. The N-domain of the human CD66a adhesion molecule is a target for Opa proteins of Neisseria meningitidis and Neisseria gonorrhoeae. Mol. Microbiol. 22:929-949.
29. Ward, J. E., and J. Lutkenhaus. 1985. Overproduction of FtsZ induces minicell formation in Escherichia coli. Cell 42:941-949.
30. Wehrli, W. 1983. Rifampin: mechanisms of action and resistance. Rev. Infect. Dis. 5:407-411.
31. B and its role in acid tolerance and virulence of Listeria monocytogenes. J. Bacteriol. 180:3650-3656.
32. Wu, Q.-L., D. Kong, K. Lam, and R. N. Husson. 1997. A mycobacterial extracytoplasmic function sigma factor involved in survival following stress. J. Bacteriol. 179:2922-2929.