Toluene diisocyanate colocalizes with tubulin on cilia of differentiated human airway epithelial cells

Toxicological Sciences

Volumn 50, Issue 1, 1999, Pages 64-71

  Lange, R W ^a   Lantz, R C ^a   Stolz, D B ^a   Watkins, S C ^a   Sundareshan, P ^a   Lemus, R ^a   Karol, M H ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

Ciliary tubulin; Occupational asthma; Pulmonary epithelial cells; TDI adducted molecules

Indexed keywords

DNA FRAGMENT; TOLUENE DIISOCYANATE; TUBULIN;

ARTICLE; CELLULAR DISTRIBUTION; CILIARY MOTILITY; CYTOTOXICITY; HUMAN; HUMAN CELL; OCCUPATIONAL ASTHMA; RESPIRATORY EPITHELIUM; SIGNAL TRANSDUCTION;

BRONCHI; CELLS, CULTURED; CILIA; DOSE-RESPONSE RELATIONSHIP, DRUG; EPITHELIUM; FLOW CYTOMETRY; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; TOLUENE 2,4-DIISOCYANATE; TUBULIN;

EID: 0032763885     PISSN: 10966080     EISSN: None     Source Type: Journal    
DOI: 10.1093/toxsci/50.1.64     Document Type: Article

References (35)

1. Arnold, R., Humbert, B., Werchau, H., Gallati H., and Konig, W. (1994). Interleukin-8, interleukin-6 and soluble tumor necrosis factor receptor type I release from a human pulmonary epithelial cell line (A549) exposed to respiratory syncytial virus. Immunology 82, 126-133.
2. Chapelin, C., Coste, A. Gilain, L., Poron, F. Verra, F., and Escudier, E. (1996). Modified epithelial cell distribution in chronic airways inflammation. Eur. Respir. J. 9, 2474-2478.
3. Choi, H.-S. H., Lesser, M. Cardozo, C., and Orlowski, M. (1990). Immunohistochemical localization of endopeptidase 24.15 in rat trachea, lung tissue, and alveolar macrophages. Am. J. Respir. Cell Mol. Biol. 3, 619-624.
4. Cunningham, A. C., Kirby, J. A., and Dark, J. H. (1993). An in vitro system to model pulmonary epithelial barrier dysfunction mediated by immune effector cells. J. Heart Lung Transplant. 12, 487-493.
5. Day, B. W., Jin, R., Basalyga, D. M., Kramarik, J. A., and Karol, M. H. (1997). Formation, solvolysis, and transcarbamoylation reactions of bis (S-glutathionyl) adducts of 2,4- and 2,6-diisocyanatoluene. Chem. Res. Toxicol. 10, 424-431.
6. Day, B. W., Jin, R., and Karol, M. H. (1996). In vitro and in vivo reactions of toluene diisocyanate isomers with guinea pig hemoglobin. Chem. Res. Toxicol. 9, 568-573.
7. de Jong, P. M., van Sterkenburg, M. A. J. A., Hesseling, S. C., Kempenaar, J. A., Mulder, A. A., Mommaas, A. M., Dijkman, J. H., and Ponec, M. (1994). Ciliogenesis in human bronchial epithelial cells cultured at the air-liquid interface. Am. J. Resp. Cell Mol. Biol. 10, 271-277.
8. Ebino, K., Kramarik, J., Lemus, R., and Karol, M. H. (1998). A mouse model for study of localized toluene diisocyanate adducts following intrabronchial administration of the chemical: inflammation and antibody production. Inhal. Toxicol. 10, 503-530.
9. Egilmez, Y., and Ilhan, M. (1992). Epithelial modulation of antigen-induced tracheal smooth muscle contractions in actively sensitized guinea-pigs. Arch. Int. Pharmacodyn. Ther. 320, 81-92.
10. Fabbri, L. M., Danieli, D., Crescioli, S., Bevilacqua, P., Meli, S., Saetta, M. and Mapp, C. E. (1988). Fatal asthma in a subject sensitized to toluene diisocyanate. Am. Rev. Respir. Dis. 137, 1494-1498.
11. Gagnaire, F., Ban, M., Cour, C., Micillino, J. C., Bonnet, P., and Hettich, D. (1997). Role of tachykinins and neutral endopeptidase in toluene diisocyanate- induced bronchial hyperresponsiveness in guinea pigs. Toxicology 116, 17-26.
12. Godfrey, R. W. A. (1997). Human airway epithelial tight junctions. Microsc. Res. Tech. 38, 488-499.
13. Gray, T. E., Guzman, K., Davis, C. W., Abdullah, L. H., and Nettesheim, P. (1996). Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 14, 104-112.
14. Guzman, K., Bader, T., and Nettesheim, P. (1996). Regulation of MUC5 and MUC1 gene expression: Correlation with airway mucous differentiation. Am. J. Physiol. 270
15. (Lung Cell. Mol. Physiol. 14), L846-L853.
16. Jin, R., Day, B. W., and Karol, M. H. (1993). Toluene diisocyanate (TDI) protein adducts in the bronchoalveolar lavage of guinea pigs exposed to vapors of the chemical. Chem. Res. Toxicol. 6, 906-912.
17. Kapsenberg, M. L. (1996). Chemicals and proteins as allergens and adjuvants. Toxicol. Lett. 86, 79-84.
18. Karol, M. H., Graham, C., Gealy, R., Macina, O. T, Sussman, N., and Rosenkranz, H. S. (1996). Structure-activity relationships and computer assisted analysis of respiratory sensitization potential. Toxicol. Lett. 86, 187-191.
19. Karol, M. H., and Jin, R. (1991). Mechanisms of immunotoxicity of isocyanates. Chem. Res. Toxicol. 4, 503-509.
20. Karol, M. H., Jin, R., and Lantz, R. C. (1997). Immunohistochemical detection of toluene diisocyanate (TDI) adducts in pulmonary tissue of guinea pigs following inhalation exposure. Inhal. Toxicol. 9, 63-83.
21. Karol, M. H., Tollerud, D. J., Campbell, T. P., Fabbri, L., Maestrelli, P., Saetta, M. and Mapp, C. E. (1994). Predictive value of airway hyperresponsiveness and circulating IgE for identifying types of responses to toluene diisocyanate inhalation challenge. Am. J. Resp. Crit. Care Med. 149, 611-615.
22. Kheradmand, F., Werner, E., Tremble, P., Symons, M., and Werb, Z. (1998). Role of Rac1 and oxygen radicals in collagenase-1 expression induced by cell shape change. Science 280, 898-902.
23. Mapp, C. E., Saetta, M., Maestrelli, P., Di Stefano, A., Chitano, P., Boschetto, P., Ciaccia, A., and Fabbri, L. M. (1994). Mechanisms and pathology of occupational asthma. Eur. Respir. J. 7, 544-554.
24. Mette, S. A., Pilewski, J., Buck, C. A., and Albeda, S. M. (1993). Distribution of integrin cell adhesion receptors on normal bronchial epithelial cells and lung cancer cells in vitro and in vivo. Am. J. Respir. Cell Mol. Biol. 8, 562-572
25. Pavia, D. (1984). Lung mucociliary clearance. In Aerosols and the Lung. (S. W. Clarke and D. Pavia, Eds.), pp. 127-155. Butterworth, London.
26. Peel, M., Marczynski, B., and Baur, X. (1997). Comparison of the binding potential of various diisocyanates on DNA in vitro. J. Toxicol. Environ. Health 52, 517-526.
27. Ravindra, R. (1997). Is signal transduction modulated by an interaction between heterotrimeric G-proteins and tubulin? Endocrinology 7, 127-143.
28. Redlich, C. A., Karol, M. H., Graham, C., Homer, R. J., Holm, C. T., Wirth, J. A., and Cullen, M. R. (1997). Airway isocyanate-adducts in asthma induced by exposure to hexamethylene diisocyanate. Scand. J. Work Environ. Health 23, 227-231.
29. Tarkington, B. K., Wu, R., Sun, W. M., Nikula, K. J., Wilson, D. W., and Last, J. A. (1994). In vitro exposure of tracheobronchial epithelial cells and of tracheal explants to ozone. Toxicology 88, 51-68.
30. Uchida, D. A., Irvin, C. G., Ballowe, C., Larsen, G., and Cott, G. R. (1996). Cationic proteins increase the permeability of cultured rabbit tracheal epithelial cells: Modification by heparin and extracellular calcium. Exp. Lung Res. 22, 85-99.
31. Weibel, E. R. (1985). Lung cell biology. In Handbook of Physiology, Section 3: The Respiratory System. (A. P. Fishman, Ed.), pp. 47-91. American Physiology Society, Bethesda, MD.
32. Wheatley, D. N., Wang, A. M., and Strugnell, G. E. (1996). Expression of primary cilia in mammalian cells. Cell. Biol. Int. 20, 73-81.
33. Widdicombe, J. H., Coleman, D. L., Finkbeiner, W. E., and Tuet, I. K. (1985). Electrical properties of monolayers cultured from cells of human tracheal mucosa. J. Appl. Physiol. 58, 1729-1735.
34. Wright, D. T., Fischer, B. M., Li, C., Rochelle, L. G., Akley, N. J., and Adler, K. B. (1996). Oxidant stress stimulates mucin secretion and PLC in airway epithelium via a nitric oxide-dependent mechanism. Am. J. Physiol. 271
35. (Lung Cell. Mol. Physiol. 15), L854-L861.