An immobilized Co2+ affinity matrix for purification of 6xhistidine-tagged proteins

American Biotechnology Laboratory

Volumn 15, Issue 1, 1997, Pages 12-14

  Yang, T E Tuan ^a   Nelson, Paul S ^a   Bush, Gay L ^b   Meyer, David I ^b   Kain, Steven R ^a  

^a CLONTECH Laboratories Inc   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 2442434736     PISSN: 07493223     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Porath J, Carlsson J, Olsson I, Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 1975; 258-598-9.
2. Ford CF, Suominen I, Glatz CE. Fusion tails for the recovery and purification of recombinant proteins. Prot Express Purif 1991; 2:95-107.
3. Hochuli E, Dobeli H, Schacher A. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J Chromatogr 1987; 411:177-84.
4. Hochuli E, Bannwarth W, Dobeli H, Gentz R, Stuber D. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 1988; 6:1321-5.
5. Bush GL, Tassin A-M, Friden H, Meyer DI. Secretion in yeast Purification and in vitro translocation of chemical amounts of prepro-α-factor. J Biol Chem 1991; 266:13811-4.