Overlapping but distinct specificities of anti-liver-kidney microsome antibodies in autoimmune hepatitis type II and hepatitis C revealed by recombinant native CYP2D6 and novel peptide epitopes

Clinical and Experimental Immunology

Volumn 118, Issue 2, 1999, Pages 290-297

  Klein, Reinhild ^c   Zanger, U M ^a   Berg, T ^b   Hopf, U ^b   Berg, P A ^d  

^a DR MARGARETE FISCHER BOSCH INSTITUTE OF CLINICAL PHARMACOLOGY   (Germany)

^b HUMBOLDT UNIVERSITY   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

^d NONE

Author keywords

Anti liver kidney microsome antibody; Autoimmune hepatitis; Cytochrome P4502D6; Hepatitis C; Immunodominant epitopes

Indexed keywords

DEBRISOQUINE 4 HYDROXYLASE; EPITOPE; MICROSOME ANTIBODY;

ADULT; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ARTICLE; AUTOIMMUNE HEPATITIS; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; FEMALE; HEPATITIS C; HUMAN; HUMAN TISSUE; LIVER MICROSOME; MALE; PRIORITY JOURNAL;

ADOLESCENT; ADULT; AGED; ANTIBODY SPECIFICITY; AUTOANTIBODIES; CHILD; CYTOCHROME P-450 CYP2D6; FEMALE; HEPATITIS C, CHRONIC; HEPATITIS, AUTOIMMUNE; HUMANS; IMMUNE SERA; IMMUNODOMINANT EPITOPES; KIDNEY; MALE; MICROSOMES, LIVER; MIDDLE AGED; PEPTIDES; RECOMBINANT PROTEINS;

EID: 0032756647     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2249.1999.01027.x     Document Type: Article

References (44)

1. 1 Rizzetto M, Swana G, Doniach D. Microsomal antibodies in active chronic hepatitis and other disorders. Clin Exp Immunol 1973; 15:331-4.
2. 2 Peakman M, Lobo-Yeo A, Mieli-Vergani G, Davies ET, Mowat AP, Vergani T. Characterization of anti-liver kidney microsomal antibody in childhood autoimmune chronic active hepatitis: evidence for IgGl subclass restriction, polyclonality and non-cross-reactivity with hepatocyte surface antigens. Clin Exp Immunol 1987; 69:543-9.
3. 3 Homberg JC, Abuaf N, Bernard O et al. Chronic active hepatitis associated with anti-liver/kidney microsome antibody type 1: a second type of 'autoimmune' hepatitis. Hepatology 1987; 6:1333-9.
4. 4 Beaune PH, Dansette PM, Mansuy D et al. Human anti-endoplasmic reticulum autoantibodies appearing in a drug-induced hepatitis are directed against a human liver cytochrome P-450 that hydroxylates the drug. Proc Natl Acad Sci USA 1987; 84:551-5.
5. 5 Crivelli O, Lavarini C, Chiaberge E, Amoroso A, Farci P, Negro F, Rizzetto M. Microsomal autoantibodies in chronic infection with the HBsAg associated delta agent. Clin Exp Immunol 1983; 54:232-8.
6. 6 Alvarez F, Bernard O, Homberg JC, Kreibich G. Anti-Liver kidney microsome antibody recognizes a 50,000 molecular weight protein of the endoplasmic reticulum. J Exp Med 1995; 161:1231-6.
7. 7 Zanger UM, Hauri HP, Loeper J, Homberg JC, Meyer UA. Antibodies against human cytochrome P450dbl in autoimmune hepatitis type II. Proc Natl Acad Sci USA 1988; 27:8256-60.
8. 8 Manns MP, Johnson EF, Griffin KJ, Tan EM, Sullivan KF. Major antigen of liver kidney microsomal autoantibodies in idiopathic autoimmune hepatitis is cytochrome P450dbl. J Clin Invest 1989; 83:1066-72.
9. 9 Gueguen M, Yamamoto AM, Bernard O, Alvarez F. Anti-liver-kidney microsome antibody type 1 recognizes human cytochrome P450dbl. Biochem Biophys Res Commun 1989; 159:542-7.
10. 10 Manns M, Zanger U, Gerken G, Sullivan KF, Meyer zum Büschenfelde KH, Meyer UA, Fichelbaum M. Patients with type II autoimmune hepatitis express functionally intact cytochrome P-450dbl that is inhibited by LKM-1 autoantibodies in vitro but not in vivo. Hepatology 1990; 12:127-32.
11. 11 Manns M, Griffin KJ, Sullivan KF, Johnson EF. LKM-1 autoantibodies recognize a short linear sequence in P450IID6, a cytochrome P-450 monooxygenase. J Clin Invest 1991; 88:1370-8.
12. 12 Gueguen M, Boniface O, Bernard O, Clerc F, Cartwright T, Alvarez F. Identification of the main epitope on human cytochrome P450IID6 recognized by anti-liver kidney rnicrosome antibody. J Autoimmun 1991; 4:607-15.
13. 13 Yamamoto AM, Cresteil D, Boniface O, Clerc FF, Alvarez F. Identification and analysis of cytochrome P450IID6 antigenic sites recognized by anti-liver-kidney microsome type-1 antibodies (LKM1). Eur J Immunol 1993; 23:1105-11.
14. 14 Lenzi M, Ballardini G, Fusconi M, Cassani F, Zauli D, Bianchi FB. Type 2 autoimmune hepatitis and hepatitis C virus infection. Lancet 1990; 335:258-9.
15. 15 Vergani D, Mieli-Vergani G. Type II autoimmune hepatitis. What is the role of hepatitis C virus? Gastroenterology 1993; 104:1870-3.
16. 16 Seelig R, Renz. M, Bunger G, Schroter H, Seelig HP. Anti-LKM-1 antibodies determined by use of recombinant P4502D6 in ELISA and Western blot and their association with anti-HCV and HCV-RNA. Clin Exp Immunol 1993; 92:373-80.
17. 17 Yamamoto AM, Crcsleil D, Homberg JC, Alvarez F. Characterization of anti-liver-kidney microsome antibody (anti-LKM1) from hepatitis C virus-positive and -negative sera. Gastroenterology 1993; 104:1762-7.
18. 18 Ma Y, Peakman M, Lobo-Yeo A, Wen L, Lenzi M, Gäken J et al. Differences in immune recognition of cytochrome P4502D6 by liver kidney microsomal (LKM) antibody in autoimmune hepatitis and chronic hepatitis C virus infection. Clin Exp Immunol 1994; 97:94-99.
19. 19 Klein R, Berg T, Hopf U, Berg PA. Differentiation between anti-liver-kidney microsome antibodies in autoimmune (AI) hepatitis (anti-LKM1) and hepatitis C (anti-LKMx) by Western blotting (WB). J Hepatol 1994; 21 (Suppl. 1):S34.
20. 20 Durazzo M, Philipp T, Van Pelt FNAM et al. Heterogeneity of liver-kidney microsomal autoantibodies in chronic hepatitis C and D virus infection. Gastroenterology 1995; 108:455-62.
21. 21 Muratori L, Lenzi M, Ma Y, Catalela M, Mieli-Vergani G, Vergani D, Bianchi FB. Heterogeneity of liver/kidney microsomal antibody type 1 in autoimmune hepatitis and hepatitis C virus related liver disease. Gut 1995; 37:406-12.
22. 22 Ma Y, Gregorio G, Gäken J, Muratori L, Bianchi FB, Mieli-Vergani G, Vergani D. Establishment of a novel radioligand assay using eucaryotically expressed cytochrome P4502D6 for the measurement of liver kidney microsomal type 1 antibody in patients with autoimmune hepatitis and hepatitis C virus infection. J Hepatol 1997; 26:1396-402.
23. 23 Johnson PJ, McFarlane IG. Meeting report: international autoimmune hepatitis group. Hepatology 1993; 18:998-1005.
24. 324Pro) expressed by recombinant baculovirus in insect cells. Naunyn-Schmiedeberg's Arch Pharmacol 1997; 355:309-18.
25. 25 Parez N, Herzog D, Jacqz-Aigrain E, Homberg JC, Alvarez F. Study of the B cell response lo cytochrome P450IID6 in sera from chronic hepatitis C patients. Clin Exp Immunol 1996; 106:336-43.
26. 26 Engvall E. Enzyme immunoassay ELISA and EMIT. Methods Enzymol 1980; 70:419-39.
27. 27 Klein R, Huizenga JR, Gips CH, Berg PA. Antimitochondrial antibody profiles in patients with primary biliary cirrhosis before orthotopic liver transplantation and liters of antimitochondrial antibody subtypes after transplantation. J Hepatol 1994; 20:181-9.
28. 28 Kronbach T, Mathys D, Gut J, Catin T, Meyer UA. High-performance liquid Chromatographic assays for bufuralol 1′-hydroxylase, debrisoquine 4-hydroxylase, and dextromethorphan O-demethylase in microsomes and purified cytochrome P-450 isozymes of human liver. Anal Biochem 1987; 162:24-32.
29. 29 König V, Bauditz J, Lobeck H et al. Hepatitis C virus reinfection in allografts after orthotopic liver transplantation. Hepatology 1992; 16:1137-43.
30. 30 Yoshiba M, Okamoto H, Mishiro S. Detection of the GBV-C hepatitis virus genome in serum from patients with fulminant hepatitis of unknown etiology. Lancet 1995; 346:1131-2.
31. 31 Berg T, Naumann U, Fukumoto T et al. GB virus C infection in patients with chronic hepatitis B and C before and after liver transplantation. Transplantation 1996; 62:711-4.
32. 32 Shimada T, Yamazaki H, Mimura M, Inui Y, Guengerich FP. Inter-individual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians. J Pharmacol Exp Therap 1994; 270:414-23.
33. 33 Jurado A, Cárdaba B, Jara P et al. Autoimmune hepatitis type 2 and hepatitis C virus infection: study of HLA antigens. J Hepatol 1997; 26:983-91.
34. 34 Miyakawa H, Kitazawa E, Abe K et al. Anti-liver-kidney-microsome-1 autoantibody in HCV-positive sera react predominantly to conformational epitope, not to linear epitope. Hep Res 1998; 10:112-20.
35. 35 Yamamoto AM, Johanet C, Duclos-Vallee JC, Bustarret FA, Alvarez F, Homherg JC, Bach JF. A new approach to cytochrome CYP2D6 antibody detection in autoimmune, hepatitis type-2 (AIH-2) and chronic hepatitis C virus (HVC) infection: a sensitive and quantitative radioligand assay. Clin Exp Immunol 1997; 108:396-400.
36. 36 Duclos-Vallée J-C, Hajoui O, Yamamoto AM, Jacqz-Aigrain E, Alvarez F. Conformational epitopes on CYP2D6 are recognized by liver/kidney microsomal antibodies. Gastroenterology 1995; 108:470-6.
37. 37 Cribb A, Nuss C, Wang R. Antipeptide antibodies against overlapping sequences differentially inhibit human CYP2D6. Drug Metabolism Disposition 1995; 23:671-5.
38. 38 Choudhuri K, Gregorio GV, Mieli-Vergani G, Vergani D. Immunological cross-reactivity to multiple autoantigens in patients with liver-kidney microsomal type 1 autoimmune hepatitis. Hepatology 1989; 28:1177-81.
39. 39 Miyakawa H, Matsushima H, Narita Y et al. Differences in antigenic sites, recognized by anti-liver-kidney microsome-1 (LKM-1) auto-antibody, between HCV-positive and HCV-negative sera in Japanese patients. J Gastroenterol 1998; 33:529-35.
40. 40 Gregorio GV, Pensati P, Iorio R, Vegnente A, Mieli-Vergani G, Vergani D. Autoantibody prevalence in children with liver disease due to chronic hepatitis C (HCV) infection. Clin Exp Immunol 1998; 112:471-6.
41. 41 Heringlake S, Tillmann HL, Cordes-Temme P, Trautwein C, Hunsmann G, Manns MP. GBV-C/HGV is not the major cause of autoimmune hepatitis. J Hepatol 1996; 25:980-4.
42. 42 Miyakawa H, Kitazawa E, Abe K et al. Chronic hepatitis C associated with anti-liver/kidney microsome-1 antibody is not a subgroup of autoimmune hepatitis. J Gastroenlerol 1997; 32:769-76.
43. 43 Muratori L, Lenzi M, Calalela M et al. Interferon therapy in liver/ kidney microsomal antibody type 1-positive patients with chronic hepatitis C. J Hepatol 1994; 21:199-203.
44. 44 Duclos-Vallee JC, Nishiola M, Hosomi N, Arima K, Leclerq A, Bach JF, Yamamoto AM. Interferon therapy in LKM-1 positive patients with chronic hepatitis C: follow-up by a quantitative radioligand assay for CYP2D6 antibody detection. J Hepatol 1998; 28:965-70.