Use of rainbow trout primary epidermal cell cultures as an alternative to immortalized cell lines in toxicity assessment: A study with nonoxynol

Environmental Toxicology and Chemistry

Volumn 18, Issue 12, 1999, Pages 2846-2850

  Dowling, Kevin ^a   Mothersill, Carmel ^a  

^a DUBLIN INSTITUTE OF TECHNOLOGY   (Ireland)

Author keywords

Goblet cells; Necrosis; Nonoxynol; Primary epidermal cell cultures Epithelium papulosum cyprini

Indexed keywords

NONOXINOL;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL IMMORTALIZATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOPATHOLOGY; CYTOTOXICITY; DOSE TIME EFFECT RELATION; EPIDERMIS CELL; EPITHELIUM CELL; GOBLET CELL; LD 50; NONHUMAN; PRIORITY JOURNAL; RAINBOW TROUT; TOXICITY TESTING;

ANIMALIA; MYKISS; ONCHORYNCHUS; ONCHORYNCHUS MYKISS; ONCORHYNCHUS MYKISS; OXY; SALMONIDAE;

EID: 0032749654     PISSN: 07307268     EISSN: None     Source Type: Journal    
DOI: 10.1002/etc.5620181227     Document Type: Article

References (34)

1. Windholz M, Budavari S, Blumetti RF, Otterbein ES, eds. 1983. The Merck Index, 10th ed. ES Merck & Co., Rahway, NJ, USA, pp 957.
2. Routledge EJ, Sumpter JA. 1996. Estrogenic activity of surfactants and some of their degeneration products assessed using a recombinant yeast screen. Environ Toxicol Chem 15:241-248.
3. Talmage SS. 1994. Environmental and Human Safety of Major Surfactants: Alcohol Ethoxylates and Alkylphenol Ethoxylates. Lewis, Boca Raton, FL, USA.
4. Muller R, Lloyd R. 1992. Sublethal and chronic effects of pollutants on freshwater fish. In Muller R, Lloyd R, eds, Fishing News by Arrangement with the Food-Agricultural Organisation of the United Nations. Blackwell Scientific, Oxford, UK.
5. Marion M, Denizeau F. 1983. Rainbow trout and human cell culture for the evaluation of the toxicity of aquatic pollutants: A study with cadmium. Aquat Toxicol 3:329-343.
6. Bols NC, Boliska SA, Dixon DG, Hodson PV, Kaiser KLE. 1985. The use of fish cell cultures as identification of contaminant toxicity to fish. Aquat Toxicol 6:147-155.
7. Segner H. 1996. Diagnostic Ecotoxicology Project proposal: Cell-based methodology to develop markers for early, sublethal effects assessment. ENVA-CT96-0223. European Communities Contract. Umweltforschungsentrum, Leipzig, Germany, p 9.
8. Mothersill C, Lyng F, Lyons M, Cottell D. 1995. Growth and differentiation of epidermal cells from the rainbow trout established as explants and maintained in various media. J Fish Biol 46:1011-1025.
9. Bloom W, Fawcett DW. 1970. A Textbook of Histology. WB Saunders, London, UK.
10. Langdon JS. 1983. Environmental management factors in disease of fish. Fish Dis Proc 106:324-353.
11. Iger Y, Loch RA, Van der Meil JC, Wendelaar Bonga SE. 1994. Effects of waterborne cadmium on the skin of the common carp (Cyprinus carpio). Arch Environ Contam Toxicol 26:342-350.
12. Freshney RI. 1994. Cloning and selection of specific cell types. In Freshney RI, Alan R, eds, Cell Culture of Animal Cells. A Manual of Basic Technique, 3rd ed. Liss, New York, NY, USA, pp 137.
13. Soto AM, Justicia H, Wray JW, Sonnenschein C. 1991. P-Nonylphenol: An estrogenic xenobiotic released from 'modified' polystyrene. Environ Health Perspect 92:167-173.
14. Jobling S, Sumpter JP. 1993. Detergent components in sewage effluent are weakly oestrogenic to fish: An in vitro study using rainbow trout (Onchorynchus mykiss) hepatocytes. Aquat Toxicol 27:361-372.
15. Jobling S, Sheanan D, Osborne JA, Matthiessen P, Sumpter JP. 1996. Inhibition of testicular growth in rainbow trout (Onchorynchus mykiss) exposed to estrogenic alkyl phenolic chemicals. Environ Toxicol Chem 15:194-202.
16. Chen TT. 1983. Identification and characterisation of estrogen responsive gene products in the liver of rainbow trout. Can J Biochem Cell Biol 61:802-810.
17. Bigliardi PL, Herron ML, Nelson RD, Dahl MV. 1998. Effects of detergents on proliferation and metabolism of human keratinocytes. Exp Dermatol 3:89-94.
18. Fijan N, Sulimanovic D, Bearzotti M. 1983. Some properties of the Epithlioma Papulosum Cyprini (EPC) cell line from carp (Cyprinus carpio). Anim Virol (Inst Pasteur) 134:207-220.
19. Weiss L. 1988. Cell and Tissue Biology. Urbam and Schwarzenberg, Munich, Germany, pp 667.
20. Schwartzman RA, Cidlowski JA. 1993. Apoptosis: The biochemistry and molecular biology of programmed cell death. Endocr Rev 14:133-151.
21. Kerr JFR, Harmon BV. 1991. Definition and incidence of apoptosis. An historical perspective. In Tomei LD, Cope Fo, eds, Apoptosis. The Molecular Basis of Cell Death. Cold Springs Harbor Laboratory, Cold Springs Harbor, NY, USA, pp 5-9.
22. Martin SJ, Green DR, Cotter T. 1994. Dicing with death: Dissecting the components of the apoptosis machinery. TIBS 19: reviews 26-30.
23. Puck TT, Marcus PI. 1995. A rapid method for viable cell titration and clone production with hela cells in tissue culture: The use of X-irradiated cells to supply conditioning factors. Proc Natl Acad Sci USA 41:432-437.
24. McSweeney JA. 1998. Investigation of the effects of nickle on the growth and survival of primary cells and immortalised cell lines of human and toeleost cell lines. PhD thesis. University of Dublin, Dublin, Ireland.
25. Lyons-Alacantara M, Tarazona JV, Mothersill C. 1996. The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals. Cell Biol Toxicol 12:29-38.
26. Shephard KL. 1994. Functions for fish mucus. Rev Fish Biol Fish 4:401-429.
27. Roy D. 1988. Statistical analysis of anionic detergent-induced changes in the goblet mucus cells of opercular epidermis and gill epithelium of Rita rita (Ham.)(Bargradal: pisces). Ecotoxicol Environ Saf 15:260-271.
28. Roy D. 1990. Detergent induced changes in the protein constituents of various cell types of opercular epidermis of Rita rita. Biomed Environ Sci 3:290-298.
29. Wester PW, Canton JH, Dormans JAMA. 1988. Pathological effects in freshwater fish Poecilla reticulata (guppy) and Oryzias latipes (medaka) following methyl bromide and sodium bromide exposure. Aquat Toxicol 12:323-343.
30. Dietrich D, Schlatter C. 1989. Aluminum toxicity to rainbow trout at low pH. Aquat Toxicol 15:197-212.
31. Wobster G. 1975. Acute toxicity of mercury and mercuric chloride for rainbow trout (Salmo gairdneri) fry and fingerlings. J Fish Res Board Can 32:2005-2013.
32. Lock RCA, Van Overbeeke AP. 1981. Effects of mercuric chloride and methylmercuric chloride on mucus secretion in rainbow trout, Salmo gairdneri Richardson. Comp Biochem Physiol 69:67-73.
33. Lyons-Alacantara M, Mooney R, Lyng F, Cottell D, Mothersill C. 1998. The effects of cadmium exposure on the morphology and function of primary cells from rainbow trout. Cell Biochem Funct 16:1-13.
34. Babich H, Borenfreund E. 1987. Aquatic pollutants tested in vitro with early passage fish cells. ATALA 15:116-122.