MboII endonuclease heat inactivation before agarose gel electrophoresis to prevent artifactual bands in restriction patterns

BioTechniques

Volumn 27, Issue 5, 1999, Pages 886-887

  Giammanco, Giovanni M ^a   Grimont, Francine ^a   Grimont, Patrick A D ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ENDONUCLEASE;

AGAR GEL ELECTROPHORESIS; ARTICLE; ARTIFACT; BACTERIUM IDENTIFICATION; DNA CLEAVAGE; ESCHERICHIA COLI; HEAT; NONHUMAN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032746794     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99275bm01     Document Type: Article

References (5)

1. Blattner, F.R., G. Plunkett III, C.A. Bloch, N.T. Perna, V. Burland, M. Riley, J. Collado-Vides, J.D. Glasner et al. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1474.
2. Garcìa-Arata, M.I., P. Gerner-Smidt, F. Baquero and A. Ibrahim. 1997. PCR-amplified 16S and 23S rDNA restriction analysis for identification of Acinetobacter strains at the DNA group level. Res. Microbiol. 148:777-784.
3. Sektas, M., T. Kaczorowski and A.J. Podhajska. 1992. Purification and properties of the MboII, a class-IIS restriction endonuclease. Nucleic Acids Res. 20:433-438.
4. Sektas, M., T. Kaczorowski and A.J. Podhajska. 1995. Interaction of the MboII restriction endonuclease with DNA. Gene 157:181-185.
5. Szybalsky, W., S.C. Kim, N. Hasan and A.J. Podhajska. 1991. Class-IIS restriction enzymes - a review. Gene 100:13-26.