Direct mecA detection from blood culture bottles by branched-DNA signal amplification

Journal of Clinical Microbiology

Volumn 37, Issue 12, 1999, Pages 4192-4193

  Zheng, X ^a,d   Kolbert, C P ^a   Varga Delmore, P ^b   Arruda, J ^b   Lewis, M ^b   Kolberg, J ^b   Cockerill, F R ^a   Persing, D H ^a,c  

^a MAYO CLINIC   (United States)

^b BAYER CORPORATION   (United States)

^c GLAXOSMITHKLINE   (United States)

^d QUEEN S MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC THERAPY; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD CULTURE; DNA DETERMINATION; GENE AMPLIFICATION; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; STAPHYLOCOCCUS;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STAPHYLOCOCCI; STAPHYLOCOCCUS;

EID: 0032745677     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.12.4192-4193.1999     Document Type: Article

References (18)

1. Ahmadinejad, M., J. W. Snyder, and M. J. Perlin. 1998. A combined molecular approach to screen for mec gene variants from methicillin-resistant Staphylococcus aureus. Diagn. Microbiol. Infect. Dis. 30:17-20.
2. Boyce, J. 1998. Are the epidemiology and microbiology of methicillin-resistant Staphylococcus aureus changing? JAMA 279:623-624.
3. Carroll, K. C., R. B. Leonard, P. L. Newcomb-Gayman, and D. R. Hillyard. 1996. Rapid detection of the staphylococcal mecA gene from BACTEC blood culture bottles by the polymerase chain reaction. Am. J. Clin. Pathol. 106:600-605.
4. de Lencastre, H., B. L. M. de Jonge, P. R. Matthews, and A. Tomasz. 1994. Molecular aspects of methicillin resistance in Staphylococcus aureus. J. Antimicrob. Chemother. 33:7-24.
5. de Lencastre, H., A. M. Sa Figueiredo, C. Urban, J. Rahal, and A. Tomasz. 1991. Multiple mechanisms of methicillin resistance and improved methods for detection in clinical isolates of Staphylococcus aureus. Antimicrob. Agents Chemother. 35:632-639.
6. Geba, D. J., J. R. Uhl, C. A. Gustaferro, and D. H. Persing. 1994. Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. J. Clin. Microbiol. 32:1768-1772.
7. Herold, B. C., L. C. Immergluck, M. C. Maranan, D. S. Lauderdale, R. E. Gaskin, S. Boyle-Vavra, C. D. Leitch, and R. S. Daum. 1998. Community-acquired methicillin-resistant Staphylococcus aureus in children with no identified predisposing risk. JAMA 279:593-598.
8. Kolbert, C. P., J. Arruda, P. Varga-Delmore, X. Zheng, M. Lewis, J. Kolberg, and D. H. Persing. 1998. Branched-DNA assay for detection of the mecA gene in oxacillin-resistant and oxacillin-sensitive staphylococci. J. Clin. Microbiol. 36:2640-2644.
9. Kolbert, C. P., J. E. Connolly, M. J. Lee, and D. H. Persing. 1995. Detection of the staphylococcal mecA gene by chemiluminescent DNA hybridization. J. Clin. Microbiol. 33:2179-2182.
10. Marshall, S. A., W. W. Wilke, M. A. Pfaller, and R. N. Jones. 1998. Staphylococcus aureus and coagulase negative staphylococci from blood stream infections: frequency of occurrence, antimicrobial susceptibility, and molecular (mecA) characterization of oxacillin resistance in the SCOPE program. Diagn. Microbiol. Infect. Dis. 30:205-214.
11. Montanari, M. P., E. Tonin, F. Biavasco, and P. E. Varaldo. 1990. Further characterization of borderline methicillin-resistant Staphylococcus aureus and analysis of penicillin-binding proteins. Antimicrob. Agents Chemother. 34:911-913.
12. Mulligan, M. E., K. A. Murray-Leisure, B. S. Ribner, H. C. Standiford, J. F. John, J. A. Korvick, C. A. Kauffman, and V. L. Yu. 1993. Methicillin-resistant Staphylococcus aureus: a consensus review of the microbiology, pathogenesis, and epidemiology with implications for prevention and management. Am. J. Med. 94:313-328.
13. Ramotar, K., M. Bobrowsks, P. Jessamine, and B. Toye. 1998. Detection of methicillin resistance in coagulase-negative staphylococci initially reported as methicillin susceptible using automated methods. Diagn. Microbiol. Infect. Dis. 30:267-273.
14. Salisbury, S. M., L. M. Sabatini, and C. A. Spiegel. 1995. Identification of methicillin-resistant staphylococci by multiplex polymerase chain reaction assay. Am. J. Clin. Pathol. 107:368-373.
15. Tomasz, A., H. B. Drugeon, H. M. de Lencastre, D. Jabes, L. McDougal, and J. Bille. 1989. New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Antimicrob. Agents Chemother. 33:1869-1874.
16. Ubukata, K., R. Nonoguchi, M. Matsuhashi, and M. Konno. 1989. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein. J. Bacteriol. 171:2882-2885.
17. York, M. K., L. Gibbs, F. Chehab, and G. F. Brooks. 1996. Comparison of PCR detection of mecA with standard susceptibility testing methods to determine methicillin resistance in coagulase-negative staphylococci. J. Clin. Microbiol. 34:249-253.
18. Zaher, A., S. Al-Thawadi, and N. Cimolai. 1997. β-lactamase negative, methicillin-resistant Staphylococcus aureus lacking the mecA gene determinant. J. Antimicrob. Chemother. 30:108-109.