Generation and monitoring of cell lines producing humanized antibodies

Clinical Cancer Research

Volumn 5, Issue 10 SUPPL., 1999, Pages

  Losman, Michele J ^a   Qu, Zhengxing ^a   Krishnan, Indira S ^a   Wang, Jingrong ^a   Hansen, Hans J ^a   Goldenberg, David M ^b   Leung, Shui On ^a  

^a IMMUNOMEDICS INC   (United States)

^b GARDEN STATE CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HUMAN MONOCLONAL ANTIBODY; IMMUNOGLOBULIN G; METHOTREXATE; MONOCLONAL ANTIBODY HMN 3; MONOCLONAL ANTIBODY HMU 9; MONOCLONAL ANTIBODY HW 12; UNCLASSIFIED DRUG;

ANIMAL CELL; ASSAY; CELL LINE; CONFERENCE PAPER; CULTURE MEDIUM; GENE AMPLIFICATION; HUMAN; IMMUNOGLOBULIN PRODUCTION; MONITORING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY;

ANIMALS; ANTIBODIES, MONOCLONAL; BLOTTING, WESTERN; CELL LINE; DNA, COMPLEMENTARY; HUMANS; METHOTREXATE; MICE;

EID: 0032740887     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (30)

1. Leung, S. O., Shevitz, J., Pelligrini, H. C., Dion, A. S., Shih, L. B., Goldenberg, D. M., and Hansen, H. J. Chimerization of LL2, a rapidly internalizing antibody specific for B-cell lymphoma. Hybridoma, 13: 469-476, 1994.
2. Leung, S. O., Goldenberg, D. M., Dion, A. S., Pelligrini, M. C., Shevitz, J., Shih, L. B., and Hansen, H. J. Construction and characterization of a humanized internalizing B-cell (CD22)-specific leukemia/lymphoma antibody, LL2. Mol. Immunol., 32: 1413-1427, 1995.
3. Juweid, M., Sharkey, R. M., Markowitz, A., Behr, T., Swayne, L. C., Dunn, R., Hansen, H. J., Shevitz, J., Leung, S. O., Rubin, A. D., Herskovic, T., Hanley, D., and Goldenberg, D. M. Treatment of non-Hodgkin's lymphoma with radiolabeled murine, chimeric, or humanized LL2, an anti-CD22 monoclonal antibody. Cancer Res., 55: 5899s-5907s, 1995.
4. Hansen, H. J., Goldenberg, D. M., Newman, E. S., Grebeneau, R., and Sharkey, R. M. Characterization of second-generation monoclonal antibodies against carcinoembryonic antigen. Cancer (Phila.), 71: 3478-3485, 1993.
5. Becker, W., Goldenberg, D. M., and Wolf, F. The use of monoclonal antibodies and antibody fragments in the imaging of infectious lesions. Semin. Nucl. Med., 24: 142-153, 1994.
6. Becker, W., Bair, J., Behr, T., Repp, R., Streckenbach, H., Beck, H., Gramatzki, M., Winship, M. J., Goldenberg, D. M., and Wolf, F. Detection of soft-tissue infections and osteomyelitis using a technetium-99m-labeled anti-granulocyte monoclonal antibody fragment. J. Nucl. Med., 35: 1436-1443, 1994.
7. Gold, D. V., Nocera, M., Stephens, R., and Goldenberg, D. M. Murine monoclonal antibodies to colon-specific antigen-p. Cancer Res., 50: 6405-6409, 1993.
8. Pant, K. D., Dahlman, H. L., and Goldenberg, D. M. A putatively new antigen (CSAp) associated with gastrointestinal and ovarian neoplasia. Immunol. Commun., 6: 411-421, 1977.
9. Pant, K. D., Dahlman, H. L., and Goldenberg, D. M. Further characterization of CSAp, an antigen associated with gastrointestinal and ovarian tumors. Cancer (Phila.), 42: 1626-1634, 1978.
10. Sharkey, R. M., Gold, D. V., Anninipot, R., Vagg, R., Ballance, C., Newman, E., Ostella, F., Hansen, H. J., and Goldenberg, D. M. Comparison of tumor targeting in nude mice of murine monoclonal antibodies directed aginst different human colorectal cancer antigens. Cancer Res., 50: 828-834, 1990.
11. Primus, F. J., Newell, K. D., Blue, A., and Goldenberg, D. M. Immunological heterogeneity of carcinoembryonic antigen: antigenic determinants on carcinoembryonic antigen distinguished by monoclonal antibodies. Cancer Res., 43: 686-692, 1983.
12. Blumenthal, R. D., Kashi, R., Stephens, R., Sharkey, R. M., and Goldenberg, D. M. Improved radioimmunotherapy of colorectal cancer xenografts using antibody mixtures against carcinoembryonic antigen and colon-specific antigen-p. Cancer Immunol. Immunother., 32: 303-310, 1991.
13. Pant, K. B., Shochat, D., Nelson, M. O., and Goldenberg, D. M. Colon-specific antigen-p (CSAp). I. Initial clinical evaluation as a marker for colorectal cancer. Cancer (Phila.), 50: 919-926, 1992.
14. Sharkey, R. M., Goldenberg, D. M., Vagg, R., Pawlyk, D., Wong, G. Y., Siegel, J. A., Murthy, S., Levine, G. M., Izon, D., Gascon, P., Burger, K., Swayne, L. C., and Hansen, H. J. Phase I clinical evaluation of a new murine monoclonal antibody (Mu-9) against colon-specific antigen-p for targeting gastrointestinal carcinomas. Cancer (Phila.), 73: 864-877, 1994.
15. Losman, M. J., Novick, K. E., Goldenberg, D. M., and Monestier, M. Mimicry of a carcinoembryonic antigen epitope by a rat monoclonal anti-idiotype antibody. Int. J. Cancer, 56: 580-584, 1994.
16. Sharkey, R. M., Goldenberg, D. M., Murthy, S., Pinsky, H., Vagg, R., Pawlyk, D., Siegel, J. A., Wong, G. Y., Gascon, P., Izon, D., Vezza, M., Burger, K., Swayne, L. C., Pinsky, C. M., and Hansen, H. J. Clinical evaluation of tumor targeting with a high-affinity anticarcinoembryonic-antigen-specific murine monoclonal antibody, MN-14. Cancer (Phila.), 71: 2082-2096, 1993.
17. 2. Cancer (Phila.), 78: 157-168, 1996.
18. Sharkey, R. M., Karaway, H., Griffiths, G. L., Behr, T. M., Blumenthal, R. D., Mattes, M. J., Hansen, H. J., and Goldenberg, D. M. Development of a streptavidin-anti-carcinoembryonic antigen antibody, radiolabeled biotin pretargeting method for radioimmunotherapy of colorectal cancer. Studies in a human colon cancer xenograft model. Bioconjugate Chem., 8: 595-604, 1997.
19. Krishnan, I. S., Hansen, H. J., Losman, M. J., Goldenberg, D. M., and Leung, S. O. Chimerization of Mu-9: a colon-specific antigen-p antibody reactive with gastrointestinal carcinomas. Cancer (Phila.), 80: 2667-2674, 1997.
20. Leung, S. O., Losman, M. J., Krishnan, I. S., Sharkey, R. M., Behr, T. M., Goldenberg, D. M., and Hansen, H. J. Humanization of an anti-carcinoembryonic antigen anti-idiotype antibody as a tumor vaccine and for targeting applications. Proc. Am. Assoc. Cancer Res., 37: 468, 1996.
21. Gillies, S. D., Lo, K. M., and Wesolowski, J. High-level expression of chimeric antibodies using adapted cDNA variable region cassettes. J. Immunol. Methods, 125: 191-202, 1989.
22. Losman, M. J., Hansen, H. J., Dworak, H., Krishnan, I. S., Qu. Z., Shih, L. B., Zeng, L., Goldenberg, D. M., and Leung, S. O. Generation of a high-producing clone of a humanized anti-B-cell lymphoma monoclonal antibody (hLL2). Cancer (Phila.), 80: 2660-2666, 1997.
23. Orlandi, R., Gussow, D. H., Jones, P. T., and Winter, G. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA. 86: 3833-3867, 1989.
24. Wahl, G. M., Robert de Saint Vincent, B., and DeRose, M. L. Effect of chromosomal position on amplification of transfected genes in animal cells. Nature (Lond.), 307: 516-520, 1984.
25. Kaufman, R. J., and Sharp, P. A. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase cDNA gene. J. Mol. Biol., 159: 601-621, 1982.
26. Ruiz, J. C., and Wahl, G. M. Formation of an inverted duplication can be an initial step in gene amplification. Mol. Cell. Biol., 8: 4302-4313, 1998.
27. Duenas, M., Ayala, M., Vazquez, J., Ohlin, M., Soderlind, E., Borrebaeck, C. A., and Gavilondo, J. V. A point of mutation in a murine immunoglobulin V-region strongly influences the antibody yield in Escherichia coli. Gene (Amst.), 158: 61-66, 1995.
28. Forsberg, G., Forsgren, M., Jaki, M., Norin, M., Sterky, C., Enhorning, A., Larsson, K., Ericsson, M., and Bjork, P. Identification of framework residues in a secreted recombinant antibody fragment that control production level and localization in Escherichia coli. J. Biol. Chem., 272: 12430-12436, 1997.
29. Chen, C., Martin, T. M., Stevens, S., and Rittenberg, M. B. Defective secretion of an immunoglobulin caused by mutations in the heavy chain complementarity-determining region 2. J. Exp. Med., 180: 577-586, 1994.
30. th ed. Bethesda, MD: United States Department of Health and Human Services, United States Government Printing Office, 1991.