Analysis of gene expression in single cells

Current Opinion in Biotechnology

Volumn 10, Issue 6, 1999, Pages 579-582

  Freeman, Tom C ^a   Lee, Kevin ^b   Richardson, Peter J ^c  

^a WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^b NEUROSCIENCE RESEARCH CENTRE   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DNA; MESSENGER RNA;

CELL FUNCTION; CELL STRUCTURE; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; NONHUMAN; PHYSIOLOGY; PRIORITY JOURNAL; SHORT SURVEY; SOMATIC CELL GENETICS;

EID: 0032736613     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(99)00036-1     Document Type: Review

References (26)

1. Ormond (Ed): Flow Cytometry: a Practical Approach. Oxford: Oxford University Press; 1994.
2. Richardson P.J., Lee K., Dixon A.K., Freeman T.C. Analysis of gene expression at the tissue and cellular level using RT-PCR. Livesey R., Hunt S.P. Differential Gene Expression: a Practical Approach. 1999;Oxford University Press, Oxford. in press.
3. Shibata D., Hawes D., Li Z.H., Hernandez A.M., Spruck C.H., Nichols P.W. Specific genetic analysis of microscopic tissue after selective ultraviolet radiation fractionation and the polymerase chain reaction. Am J Pathol. 141:1992;539-543.
4. Becker I., Becker K.F., Rohrl M.H., Minkus G., Schutze K., Hofler H. Single-cell mutation analysis of tumors from stained histologic slides. Lab Invest. 75:1996;801-807.
5. Whetsell L., Maw G., Nadon N., Ringer D.P., Schaefer F.V. Polymerase chain reaction microanalysis of tumors from stained histological slides. Oncogene. 7:1992;2355-2361.
6. Emmert-Buck M.R., Bonner R.F., Smith P.D., Chuaqui R.F., Zhuang Z., Goldstein S.R., Weiss R.A., Liotta L.A. Laser capture microdissection. Science. 274:1996;998-1001.
7. Van Gelder R.N., Von Zastrow M.E., Yool A., Dement W.C., Barchas J.D., Eberwine J.H. Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA. 87:1990;1663-1667.
8. Ghasemzadeh M.B., Sharma S., Surmeier D.J., Eberwine J.H., Chesselet M.F. Multiplicity of glutamate receptor subunits in single striatal neurons: an RNA amplification study. Mol Pharmacol. 49:1996;852-859.
9. Ginsberg S.D., Crino P.B., Hemby S.E., Weingarten J.A., Lee V.M.Y., Eberwine J.H., Trojanowski J.Q. Predominance of neuronal mRNAs in individual Alzheimer's disease senile plaques. Ann Neurol. 45:1999;174-181. The power of RNA amplification is seen in its ability to detect mRNA within senile plaques.
10. Crino P.B., Eberwine J. Molecular characterization of the dendritic growth cone: regulated mRNA transport and local protein synthesis. Neuron. 17:1996;1173-1187.
11. Crino P., Khodakhah K., Becker K., Ginsberg S., Hemby S., Eberwine J. Presence and phosphorylation of transcription factors in developing dendrites. Proc Natl Acad Sci USA. 95:1998;2313-2318. The sensitivity of RNA amplification is shown in its ability to detect gene expression in single dendrites.
12. Cao Y.X., Wilcox K.S., Martin C.E., Rachinsky T.L., Eberwine J., Dichter M.A. Presence of mRNA for glutamic acid decarboxylase in both excitatory and inhibitory neurons. Proc Natl Acad Sci USA. 93:1996;9844-9849.
13. Lambolez B., Audinat E., Bochet P., Crepel F., Rossier J. AMPA receptor subunits expressed by single Purkinje cells. Neuron. 9:1992;247-258.
14. Sucher N.J., Deitcher D.L. PCR and patch-clamp analysis of single neurons. Neuron. 14:1995;1095-1100.
15. Bochet P., Audinat E., Lambolez B., Crepel F., Rossier J., Iino M., Tsuzuki K., Ozawa S. Subunit composition at the single-cell level explains functional properties of a glutamate-gated channel. Neuron. 12:1994;383-388.
16. Massengill J.L., Smith M.A., Son D.I., O'Dowd D.K. Differential expression of K4-AP currents and Kv3.1 potassium channel transcripts in cortical neurons that develop distinct firing phenotypes. J Neurosci. 17:1997;3136-3147.
17. 2+ currents in rat neostriatal cholinergic interneurons through a fast, membrane delimited, G-protein pathway. J Neurosci. 16:1996;2592-2604.
18. •] analyse, using nested PCR, the expression of specific genes in separate nested amplifications using samples from single neurons and relate cell function to gene expression.
19. Mermelstein P.G., Song W.J., Tkatch T., Yan Z., Surmeier D.J. Inwardly rectifying potassium (IRK) currents are correlated with IRK subunit expression in rat nucleus accumbens medium spiny neurons. J Neurosci. 18:1998;6650-6661. Another example of how to relate cell function to gene expression.
20. Dixon A., Richardson K., Lee P.J., Carter K., Freeman T.C., Freeman N.P. Expression profiling of single cells using three prime end amplification (TPEA) PCR. Nucleic Acid Res. 26:1998;4426-4431. Global amplification by PCR on FACS sorted single cells and samples aspirated from single neurons. As this method permits global amplification of all mRNAs it will be suited to the amplification of samples for subsequent analysis on microarrays.
21. Lee K., Dixon A.K., Freeman T.C., Richardson P.J. Identification of an ATP sensitive potassium channel current in rat striatal cholinergic interneurons. J Physiol. 510:1998;441-453.
22. Richardson P.J., Dixon A.K., Lee K., Bell M.I., Cox P.J., Williams R., Pinnock R.D., Freeman T.C. Correlating cell physiology with gene expression in single neurons. J Neurochem. 1999;. in press.
23. 3H]-acetylcholine release in mouse striatum through multiple receptor subtypes. Neurosci. 1999;. in press.
24. Lockhart D.J., Dong H., Byrne M.C., Follettie M.T., Gallo M.V., Chee M.S., Mittmann M., Wang C., Kobayashi M., Horton H. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat Biotechnol. 14:1996;1675-1680.
25. Schena M., Shalon D., Davis R.W., Brown P.O. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science. 270:1995;467-470.
26. Luo L., Salunga R.C., Guo H.Q., Bittner A., Joy K.C., Galindo J.E., Xiao H.N., Rogers K.E., Wan J.S., Jackson M.R. Gene expression profiles of laser-captured adjacent neuronal subtypes. Nat Med. 5:1999;117-122. The potential of laser capture microdissection as a means of obtaining cells and analysing their expression on microarrays. 1000 cells were used and the expression of 477 genes analysed.