A simplified and efficient method for obtaining metaphase chromosomes from individual human blastomeres

Fertility and Sterility

Volumn 72, Issue 6, 1999, Pages 1127-1133

  Verlinsky, Yury ^a   Evsikov, Sergei ^a  

^a REPRODUCTIVE GENETICS INSTITUTE   (United States)

Author keywords

Chromosomal translocations; Karyotyping; Preimplantation genetic diagnosis; Visualization of chromosomes; Whole chromosome painting

Indexed keywords

ARTICLE; CHROMOSOME CONDENSATION; CHROMOSOME TRANSLOCATION; FERTILIZATION IN VITRO; HUMAN; HUMAN CELL; KARYOTYPING; METAPHASE CHROMOSOME; PRENATAL DIAGNOSIS; PRIORITY JOURNAL;

EID: 0032729275     PISSN: 00150282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0015-0282(99)00394-5     Document Type: Note

References (16)

1. Ménézo Y.J.R., Bellec V., Zaroukian A., Benkhalifa M. Embryo selection by IVF, co-culture and transfer at the blastocyst stage in case of translocation. Hum Reprod. 12:1997;2802-2803.
2. Pierce K.E., Fitzgerald L.M., Seibel M.M., Zilberstein M. Preimplantation genetic diagnosis of chromosomal balance in embryos from a patient with a balanced reciprocal translocation. Mol Hum Reprod. 4:1998;167-172.
3. Iwarsson E., Ährlund-Richter L., Inzunza J., Rosenlund B., Fridström M., Hillensjö T., et al. Preimplantation genetic diagnosis of a large pericentric inversion of chromosome 5. Mol Hum Reprod. 4:1998;719-723.
4. Munné S., Scott R., Sable D., Cohen J. First pregnancies after preconception diagnosis of translocations of maternal origin. Fertil Steril. 69:1998;675-681.
5. Conn C.M., Harper J.C., Winston R.M.L., Delhanty J.D.A. Infertile couples with robertsonian translocations preimplantation genetic analysis of embryos reveals chaotic cleavage divisions . Hum Genet. 102:1998;117-123.
6. Munné S., Morrison L., Fung J., Márquez C., Weier U., Bahçe M., et al. Spontaneous abortions are reduced after preconception diagnosis of translocations. J Assist Reprod Genet. 15:1998;290-296.
7. Verlinsky Y., Evsikov S. Karyotyping of human oocytes by chromosomal analysis of the second polar bodies. Mol Hum Reprod. 5:1999;89-95.
8. Evsikov S., Verlinsky Y. Visualization of chromosomes in single human blastomeres. J Assist Reprod Genet. 16:1999;133-137.
9. Tsunoda Y., Yasui T., Nakamura K., Uchida T., Sugie T. Effect of cutting the zona pellucida on the pronuclear transplantation in the mouse. J Exp Zool. 240:1986;119-125.
10. Cieslak J., Ivakhnenko V., Wolf G., Sheleg S., Verlinsky Y. Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching. Fertil Steril. 71:1999;308-313.
11. Czolwska R., Tarkowski A. First meiosis of early dictyate nuclei from primordial oocytes in mature and activated mouse oocytes. Zygote. 4:1996;73-80.
12. Dyban A.P. An improved method for chromosome preparation from preimplantation mammalian embryos, oocytes or isolated blastomeres. Stain Technol. 58:1983;69-72.
13. Campbell K.H.S., Loi P., Otaegui P.J., Wilmut I. Cell cycle co-ordination in embryo cloning by nuclear transfer. Rev Reprod. 1:1996;40-46.
14. Campbell K.H.S. Maternal effects upon the development of embryos created by nuclear transfer. Lauria A., Gandolfi F., Enne G., Gianaroli L. Gametes development and function . 1998;439-456 Serono Symposia, Rome.
15. Willadsen S., Levron J., Munné S., Schimmel T., Márquez C., Scott R., et al. Rapid visualization of metaphase chromosomes in single human blastomeres after fusion with in-vitro matured bovine eggs. Hum Reprod. 14:1999;470-475.
16. Kamiguchi Y., Mikamo K. An improved, efficient method for analyzing human sperm chromosomes using zona-free hamster ova. Am J Hum Genet. 38:1986;724-740.