Visualization of chromosomes in single human blastomeres

Journal of Assisted Reproduction and Genetics

Volumn 16, Issue 3, 1999, Pages 133-137

  Evsikov, Sergei ^a   Verlinsky, Yury ^a  

^a REPRODUCTIVE GENETICS INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BLASTOMERE; CHROMOSOME ANALYSIS; CHROMOSOME TRANSLOCATION; CONTROLLED STUDY; CYTOPLASM; EMBRYO; EMBRYO CULTURE; FERTILIZATION IN VITRO; HAMSTER; HUMAN; HUMAN CELL; METAPHASE CHROMOSOME; MOUSE; NONHUMAN; OOCYTE; PREIMPLANTATION EMBRYO; PREMATURE CHROMOSOME CONDENSATION; PRIORITY JOURNAL; ZYGOTE;

ANIMALS; BLASTOMERES; CHROMOSOME ABERRATIONS; CHROMOSOME DISORDERS; CHROMOSOME PAINTING; CHROMOSOMES; CRICETINAE; ENZYME INHIBITORS; FEMALE; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MALE; METAPHASE; MICE; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; OKADAIC ACID; PREGNANCY; PREIMPLANTATION DIAGNOSIS;

ANIMALIA; CRICETINAE;

EID: 0033017394     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022579731014     Document Type: Article

References (16)

1. Verlinsky Y, Ginsberg N, Lifchez A, Valle J, Moise J, Strom CM: Analysis of the first polar body: Preconception genetic diagnosis. Hum Reprod 1990;5:826-829
2. Hardy K, Martin KL, Leese HJ, Winston RM, Handyside AH: Human preimplantation development in vitro is not adversely affected by biopsy at the 8-cell stage. Hum Reprod 1990;5: 708-714
3. Durban M, Benet J, Sarquella J, Egozcue J, Navarro J: Chromosome studies in first polar bodies from hamster and human oocytes. Hum Reprod 1998;13:583-587
4. Munné S, Scott R, Sable D, Cohen J: First pregnancies after preconception diagnosis of translocations of maternal origin. Fertil Steril 1998;69:675-681
5. Campbell KHS: Cloning Dolly: implications for human medicine. In Fertility and Reproductive Medicine, RD Kempers, et al. (eds). Amsterdam, Elsevier, 1988, pp 3-11
6. Tsunoda Y, Yasui T, Nakamura K, Uchida T, Sugie T: Effect of cutting the zona pellucida on the pronuclear transplantation in the mouse. J Exp Zool 1986;240:119-125
7. Tsunoda Y, Shioda M, Onodera K, Nakamura K, Uchida T: Differential sensitivity of mouse pronuclei and zygote cytoplasm to Hoechst staining and ultraviolet irradiation. J Reprod Fertil 1988;82:173-178
8. Verlinsky Y, Evsikov S: Karyotyping of human oocytes by chromosomal analysis of the second polar bodies. Mol Hum Reprod 1999 (in press)
9. Kato Y, Tsunoda Y: Synchronous division of mouse two-cell embryos with nocodazole in vitro. J Reprod Fertil 1992;95: 39-43
10. Santaló J, Veiga A, Calafell JM, Calderon G, Vidal F, Barri PN, Giménes C, Egozcue J: Evaluation of cytogenetic analysis for clinical preimplantation diagnosis. Fertil Steril 1995;64: 44-50
11. Kamiguchi Y, Mikamo K: An improved, efficient method for analyzing human sperm chromosomes using zona-free hamster ova. Am J Hum Genet 1986;38:724-740
12. Dyban AP: An improved method for chromosome preparation from preimplantation mammalian embryos, oocytes or isolated blastomeres. Stain Technol 1983;58:69-72
13. Steinmann KE, Belinsky GS, Lee D, Schlegel R: Chemically induced premature mitosis: Differential response in rodent and human cells and the relationship to cyclin B synthesis and p34 (cdc2)/cyclin B complex formation. Proc Natl Acad Sci USA 1991;88:6843-6847
14. Dyban AP, De Sutter P, Dozortsev D, Verlinsky Y: Visualization of second polar body chromosomes in fertilized and artificially activated mouse oocytes treated with okadaic acid. J Assist Reprod Genet 1992;9:572-579
15. Chesnel F, Eppig JJ: Induction of precocious germinal vesicle breakdown (GVB) by GVB-incompetem mouse oocytes: Possible role of mitogen-activated protein kinases rather than p34(cdc2) kinase. Biol Reprod 1995;52:895-902
16. Rao PN: The phenomenon of premature chromosome condensation. In Premature Chromosome Condensation: Application in Basic, Clinical, and Mutation Research, PN Rao et al. (eds). New York, Academic Press, 1982, pp 1-41