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1842269193
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note
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3 [bis-(sulfosuccinimidyl) suberate] (Pierce, Rockford, IL). Details of the synthesis are reported by J. H. Home and T. Meyer (in preparation).
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7
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0030056047
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RBL cells were grown as described [E. Oancea and T. Meyer, J. Biol. Chem. 271, 17253 (1996)]. Cells were loaded with CAAX green by means of a small-volume electroporation device (M. Teruel and T. Meyer, in preparation) 6 to 8 hours before experiments. The final concentration of CAAX green in electroporated cells was estimated to be 50 μM. Differential interference contrast and confocal fluorescence images were recorded on a Zeiss confocal microscope.
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J. Biol. Chem.
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Oancea, E.1
Meyer, T.2
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10
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1842274076
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note
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The time course of CAAX green processing was determined in digitonin-permeabilized cells (15 μg/ml). Total cell fluorescence was determined from background-subtracted images with Image-1 software. For comparison between experiments, the data were expressed as a percentage of the maximal fluorescence retained, which varied between 60 and 80% of the fluorescence before digitonin permeabilization. The incomplete retention during permeabilization is most likely due to a partial digitonin destruction of cells.
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11
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0024559489
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2+ concentration was calibrated as described [R. Y. Tsien, ibid. 19, 2396 (1980)].
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Biochemistry
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Walker, J.W.1
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12
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0019333905
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2+ concentration was calibrated as described [R. Y. Tsien, ibid. 19, 2396 (1980)].
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Biochemistry
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Tsien, R.Y.1
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13
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1842359731
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note
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2+ increases but not decreases during the time interval.
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15
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0028913955
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Y. Yao, J. Choi, I. Parker, J. Physiol. 482, 533 (1995); P. Thorn, A. M. Lawrie, P. M. Smith, V. D. Gallacher, O. H. Petersen, Cell 74, 661 (1993).
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Choi, J.2
Parker, I.3
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Y. Yao, J. Choi, I. Parker, J. Physiol. 482, 533 (1995); P. Thorn, A. M. Lawrie, P. M. Smith, V. D. Gallacher, O. H. Petersen, Cell 74, 661 (1993).
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Thorn, P.1
Lawrie, A.M.2
Smith, P.M.3
Gallacher, V.D.4
Petersen, O.H.5
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18
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0026438544
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M. Iino and M. Endo, ibid. 360, 76 (1992); E. A. Finch, T. J. Turner, S. M. Goldin, Science 252, 443 (1991); I. Bezprozvanny, J. Watras, B. E. Ehrlich, Nature 351, 751 (1991); J. H. Horne and T. Meyer, Biochemistry 34, 12738 (1995).
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Nature
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Iino, M.1
Endo, M.2
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19
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0025890114
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M. Iino and M. Endo, ibid. 360, 76 (1992); E. A. Finch, T. J. Turner, S. M. Goldin, Science 252, 443 (1991); I. Bezprozvanny, J. Watras, B. E. Ehrlich, Nature 351, 751 (1991); J. H. Horne and T. Meyer, Biochemistry 34, 12738 (1995).
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Finch, E.A.1
Turner, T.J.2
Goldin, S.M.3
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20
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0026432666
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M. Iino and M. Endo, ibid. 360, 76 (1992); E. A. Finch, T. J. Turner, S. M. Goldin, Science 252, 443 (1991); I. Bezprozvanny, J. Watras, B. E. Ehrlich, Nature 351, 751 (1991); J. H. Horne and T. Meyer, Biochemistry 34, 12738 (1995).
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Nature
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Bezprozvanny, I.1
Watras, J.2
Ehrlich, B.E.3
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21
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0028801206
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M. Iino and M. Endo, ibid. 360, 76 (1992); E. A. Finch, T. J. Turner, S. M. Goldin, Science 252, 443 (1991); I. Bezprozvanny, J. Watras, B. E. Ehrlich, Nature 351, 751 (1991); J. H. Horne and T. Meyer, Biochemistry 34, 12738 (1995).
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Biochemistry
, vol.34
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Horne, J.H.1
Meyer, T.2
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24
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1842309669
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note
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3 was performed in the laboratory of J. Walker (University of Wisconsin, Madison, WI; supported by NIH grant P01-HL-47053). Supported by NIH grants RO1-GM-48113 and GM-51457.
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