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Volumn 276, Issue 5319, 1997, Pages 1690-1693

Elementary calcium-release units induced by inositol trisphosphate

Author keywords

[No Author keywords available]

Indexed keywords

EGTAZIC ACID; INOSITOL 1,4,5 TRISPHOSPHATE; INOSITOL TRISPHOSPHATE;

EID: 0030816488     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5319.1690     Document Type: Article
Times cited : (92)

References (24)
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    • note
    • 3 [bis-(sulfosuccinimidyl) suberate] (Pierce, Rockford, IL). Details of the synthesis are reported by J. H. Home and T. Meyer (in preparation).
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    • RBL cells were grown as described [E. Oancea and T. Meyer, J. Biol. Chem. 271, 17253 (1996)]. Cells were loaded with CAAX green by means of a small-volume electroporation device (M. Teruel and T. Meyer, in preparation) 6 to 8 hours before experiments. The final concentration of CAAX green in electroporated cells was estimated to be 50 μM. Differential interference contrast and confocal fluorescence images were recorded on a Zeiss confocal microscope.
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    • The time course of CAAX green processing was determined in digitonin-permeabilized cells (15 μg/ml). Total cell fluorescence was determined from background-subtracted images with Image-1 software. For comparison between experiments, the data were expressed as a percentage of the maximal fluorescence retained, which varied between 60 and 80% of the fluorescence before digitonin permeabilization. The incomplete retention during permeabilization is most likely due to a partial digitonin destruction of cells.
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    • 2+ increases but not decreases during the time interval.
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    • note
    • 3 was performed in the laboratory of J. Walker (University of Wisconsin, Madison, WI; supported by NIH grant P01-HL-47053). Supported by NIH grants RO1-GM-48113 and GM-51457.


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