FSH immunoneutralization acutely impairs spermatogonial development in normal adult rats

Journal of Andrology

Volumn 20, Issue 6, 1999, Pages 756-762

  Meachem, S J ^a,b   McLachlan, R I ^a   Stanton, P G ^a   Robertson, D M ^a   Wreford, N G ^b  

^a MONASH MEDICAL CENTRE   (Australia)

^b MONASH UNIVERSITY   (Australia)

Author keywords

Germ cells; Maintenance; Optical disector; Passive immunization; Stereology

Indexed keywords

FOLLITROPIN; IMMUNOGLOBULIN; POLYCLONAL ANTIBODY;

ANIMAL EXPERIMENT; ARTICLE; CELL COUNT; CELL POPULATION; CELL SURVIVAL; CONTROLLED STUDY; GERM CELL; MALE; NONHUMAN; PACHYTENE; PRIORITY JOURNAL; RAT; SEMINIFEROUS TUBULE; SPERMATID; SPERMATOCYTE; SPERMATOGENESIS; SPERMATOGONIUM; STEREOLOGY; TESTIS DEVELOPMENT;

ANIMALS; ANTIBODIES; APOPTOSIS; FOLLICLE STIMULATING HORMONE; IMMUNIZATION, PASSIVE; IN SITU NICK-END LABELING; MALE; RATS; RATS, SPRAGUE-DAWLEY; SHEEP; SPERMATOCYTES; SPERMATOGONIA; TESTIS;

EID: 0032727733     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Awoniyi CA, Santulli R, Chandrashekar V, Schanbacher BD, Zirkin BR. Quantitative restoration of advanced spermatogenic cells in adult male rats made azoospermic by active immunization against luteinising hormone or gonadotrophin-releasing hormone. Endocrinology 1989; 125:1303-1309.
2. Bartlett JMS, Weinbauer GF, Nieschlag E. Differential effects of follicle-stimulating hormone and testosterone on the maintenance of spermatogenesis in the adult hypophysectomised rat. J Endocr 1989;121: 49-58.
3. Chandolia RK, Weinbauer GF, Fingscheidt U, Bartlett JMS, Nieschlag E. Effects of flutamide on testicular involution induced by an antagonist of gonadotrophin-releasing hormone and on stimulation of spermatogenesis by follicle-stimulating hormone in rats. J Reprod Fert 1991; 93:313-323.
4. De Franca LR, Jackson A, Russell LD. Hormone sensitivity of germ cells in stage XIV and in Stage I of the rat spermatogenic cycle. Tissue Cell 1994;26:375-383.
5. Dym M, Raj M, Lin YC, Chemes HE, Kotite NJ, Nayfeh SN, French FS. Is FSH required for the maintenance of spermatogenesis in adult rats? J Reprod Fert (Suppl) 1979;26:175-181.
6. Graf KM, Dias JA, Griswold MA. Decreased spermatogenesis as the result of an induced autoimmune reaction directed against the gonadotrophin receptors in male rats. J Androl 1997;18:174-185.
7. Hasegawa M, Wilson G, Russell LD, Meistrich ML. Radiation-induced cell death in the mouse testis: relationship to apoptosis. Radiat Res 1997;147:457-467.
8. Heckert LL, Griswold MD. Expression of follicle-stimulating hormone receptor mRNA in rat testes and Sertoli cells. Mol Endocrinol 1991; 5:670-677.
9. Henriksen K, Kangasniemi M, Parvinen M, Kaipa A, Hakovirta H. In vitro, follicle-stimulating hormone prevents apoptosis and stimulates deoxyribonucleic acid synthesis in the rat seminiferous epithelium in a stage-specific fashion. Endocrinology 1996;137:2140-2149.
10. Kangasniemi M, Kaipa A, Toppari J, Perheentupa A, Huhtaniemi IT, Parvinen M. Cellular regulation of FSH binding in rat seminiferous tubules. J Androl 1990;11:336-343.
11. Kerr JB, Maddocks S, Sharpe RM. Testosterone and FSH have independent, synergistic, and stage-dependent effects upon spermatogenesis in the rat testis. Cell Tissue Research 1992;268:179-189.
12. Kumar TR, Wang Y, Lu N, Matzuk M. Follicle stimulating is required for ovarian follicle maturation but not male fertility. Nature Genetics 1997;15:201-204.
13. Louis BG, Fritz IB. Follicle-stimulating hormone and testosterone independently increase the production of androgen-binding protein by Sertoli cells in culture. Endocrinology 1979;104:454-460.
14. McKinney M, Parkinson AA. Simple, nonchromatographic procedure to purify immunoglobulins from serum and ascites fluid. J Immunological Methods 1987;96:271-278.
15. McLachlan RI, Wreford NG, de Kretser DM, Robertson DM. The effects of recombinant human FSH on the restoration of spermatogenesis in the GnRH immunized adult rat. Endocrinology 1995;136:4035-4043.
16. McLachlan RI, Wreford NG, Meachem SJ, De Kretser DM, Robertson DM. Effects of testosterone on spermatogenic cell populations in the adult rat. Biol Reprod 1994a;51:945-955.
17. McLachlan RI, Wreford NG, Tsonis C, de Kretser DM, Robertson DM. Testosterone effects on spermatogenesis in the gonadotrophin-releasing hormone-immunised rat. Biol Reprod 1994b;54:36-44.
18. Meachem SJ, McLachlan RI, deKretser DM, Robertson DM, Wreford NG. Neonatal exposure of rats to recombinant human follicle-stimulating hormone increases adult Sertoli and spermatogenic cell numbers. Biol Reprod 1996;54:36-44.
19. Meachem SJ, Wreford NG, Roberston DM, McLachlan RI. Androgen action on the restoration of spermatogenesis in adult rats: effects of human chorionic gonadotrophin, testosterone, and flutamide administration on germ cell number. Int 3 Andrology 1997;20:70-79.
20. Meachem SJ, Wreford NO, Stanton PG, Robertson DM, McLachlan RI. FSH is required for the initial phase of spermatogenic restoration in adult rats following gonadotropin suppression. J Androl 1998;19:725-735.
21. Muffly KE, Nazian SJ, Cameron DF. Effects of follicle-stimulating hormone on the junction-related Sertoli cell cytoskeleton and daily sperm production in testosterone-treated hypophysectomized rats. Biol Reprod 1994;51:158-166.
22. O'Donnell L, McLachlan RI, Wreford NG, Robertson DM. Testosterone promotes the conversion of round spermatids between stages VII-VIII of the rat spermatogenic cycle. Endocrinology 1994;135:2608-2614.
23. Parvinen M, Marana R, Robertson DM, Hansson V, Ritszen EM. Functional cycle of rat Sertoli cells: differential binding and action of follicle-stimulating hormone at various stages of the spermatogenic cycle. In: Steinberger A, Steinberger E, eds. Testicular Development, Structure and Function. New York: Raven Press; 1980:425-432.
24. Raj MHG, Dym M. The effects of selective withdrawal of FSH or LH on spermatogenesis in the immature rat. Biol Reprod 1976;14:489-494.
25. Russell LD, Attlin R, Sinha Hikim A, Clegg E. Staging for laboratory species. In: Russell LD, Ettlin RA, Sinha Hikim AP. Clegg ED, eds. Histological and Histopathological Evaluation of the Testis. Florida: Cache River Press; 1990:62-118.
26. Russell LD, Corbin TJ, Borg KE, de Franca LR, Grasso P, Bartke A. Recombinant human follicle-stimulating hormone is capable of exerting a biological effect in the adult hypophysectomized rat by reducing the numbers of degenerating cells. Endocrinology 1993;133: 2062-2070.
27. Sharpe RM, Atanassova N, McKinnell C, Parte P, Turner PJ, Fisher KJ, Kerr JB, Groome NP, Macpherson S, Millar MR, Saunders PTK. Abnormalities in functional development of the Sertoli cell in rats treated neonatally with diethylstilbestrol: a possible role for estrogens in Serloli cell development. Biol Reprod 1998;59:1084-1094.
28. Shennawy EI, Gates RJ, Russell LD. Hormonal regulation of spermatogenesis in the hypophysectomized rat: cell viability after hormonal replacement in adults after intermediate periods of hypophysectomy. J Androl 1998;19:320-334.
29. Shetty J, Marathe GP, Digne RR. Specific immunoneutralization of FSH leads to apoptotic cell death of the pachytene spermatocytes and spermatogonial cells in the rat. Endocrinology 1996;137:2179-2182.
30. Sinha Hikim AP, Swerdloff RS. Temporal and stage-specific effects of recombinant human follicle-stimulating hormone on the maintenance of spermatogenesis in gonadotrophin-releasing hormone antagonisttreated rat. Endocrinology 1995;136:253-261.
31. Verhoeven G, Callieau J. Follicle-stimulating hormone and androgens increase the concentration of the androgen receptor in Sertoli cell. Endocrinology 1988;122:1541-1550.
32. Wreford NG. Theory and practice of stereological techniques applied to the estimation of cell number and nuclear volume in the testis. Microsc Res Tech 1995;32:423-436.