Immunoprecipitation analysis of pathological autoantibodies in Graves' patients' sera using biotinylated human thyrotropin receptor labeled with 125I-neutravidiny

Experimental and Clinical Endocrinology and Diabetes

Volumn 107, Issue 8, 1999, Pages 555-560

  Minich, W B ^b   Weymayer, J D ^b   Loos, Ulrich ^a  

^a UNIVERSITY OF ULM   (Germany)

^b NONE

Author keywords

Autoantibodies; Graves' disease; Immunoprecipitation; TSH receptor; Vaccinia virus

Indexed keywords

ACETYL COENZYME A CARBOXYLASE; AGAROSE; AUTOANTIBODY; AVIDIN; BIOTIN DERIVATIVE; CARBOXYL GROUP; CARRIER PROTEIN; HISTIDINE; HYBRID PROTEIN; IODINE 125; NEUTRAVIDIN; NICKEL; RECOMBINANT VACCINE; THYROTROPIN RECEPTOR; UNCLASSIFIED DRUG; VACCINIA VACCINE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; GRAVES DISEASE; HASHIMOTO DISEASE; HELA CELL; HUMAN; HUMAN CELL; IMMUNOASSAY; INSULIN DEPENDENT DIABETES MELLITUS; PRIORITY JOURNAL; PROTEIN IMMOBILIZATION; RADIOIMMUNOPRECIPITATION; RECEPTOR BINDING; RECEPTOR BLOCKING;

EID: 0032724732     PISSN: 09477349     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0029-1232565     Document Type: Article

References (11)

1. Costagliola S, Morgenthaler NG, Hoermann R, Badenhoop K, Struck J, Freitag R, Poertl S, Weglöhner W, Hollidt JM, Quadbeck B, Dumont JE, Schumm-Draeger PM, Bergmann A, Mann K, Vassart G, Usadel K-H: Second generation assay for thyrotropin receptor antibodies has superior diagnostic sensetivity for Graves' disease. J Clin Endocrinol Metab 84: 90-97, 1999
2. Hiller Y, Gershoni JM, Bayer EA, Wilchek M: Biotin binding to avidin. Oligisaccharide side chain not required for ligand association. Biochem J 248: 167-171, 1987
3. Kohn LD, Shimura H, Shimura Y, Hidaka, Giulia C, Napolitano G, Ohmor M, Laglia G, Saji M: The thyrotropin receptor. Vitam Horm 50: 287-384, 1995
4. Metzger C, Michel D, Lüske A, Schlicht HJ, Mertens T: Human cytomegalovirus UL97 kinase confers ganciclovir susceptibility to recombinant vaccinia virus. J Virol 68: 8423-8427, 1994
5. Minich WB, Behr M, Loos U: Expression of a functional tagged human thyrotropin receptor in HeLa cells using recombinant vaccinia virus. Exp Clin Endocrinol Diabetes 5: 282-290, 1997
6. Minich WB, Loos U: Isolation of radiochemically pure 125I-labeled human thyrotropin receptor and its use for the detection of pathological autoantibodies in Graves' patients' sera. J Endocrinol 160: 239-245, 1999
7. Minich WB, Weymayer JD, Loos U: Expression of a biotinylated human thyrotropin receptor in HeLa cells using recombinant vaccinia virus and its application for the detection of Graves' autoantibodies. Thyroid 8 :3-7, 1998
8. Rapoport B, Chasenbalk GD, Jaume JC, McLachlam SM: The thyrotropin (TSH)-hormone receptor: interaction with TSH and autoantibodies. Endocrinol Rev 19: 673-716, 1998
9. Refetoff S, Sunthornthepvarakul T, Gottschalk ME, Hayashi Y: Resistance to thyrotropin and other abnormalities of the thyrotropin receptor. Recent Prog Horm Res 51: 97-122, 1996
10. Shewring G, Rees Smith B: An improved radioreceptor assay for TSH receptor antibodies. Clin Endocrinol 17: 409-414, 1982
11. Tonacchera M, Van Sande J, Parma J, Duprez L, Cetani F, Costagliola S, Dumont JE, Vassart G: TSH receptor and disease. Clin Endocrinol 44: 621-633, 1996