Random transposition by Tn916 in Desulfitobacterium dehalogenans allows for isolation and characterization of halorespiration-deficient mutants

Journal of Bacteriology

Volumn 181, Issue 22, 1999, Pages 6882-6888

  Smidt, Hauke ^a   Song, Donglin ^a,b   Van Der Oost, John ^a   De Vos, Willem M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b WUHAN INSTITUTE OF VIROLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

FUMARIC ACID; HALOGENATED HYDROCARBON; MANDELIC ACID DERIVATIVE; NITRATE; STREPTOMYCIN;

ARTICLE; BACTERIUM MUTANT; ELECTRON TRANSPORT; ENTEROCOCCUS FAECALIS; ENZYME ACTIVITY; GENE DISRUPTION; GENE INSERTION; GRAM POSITIVE BACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RESPIRATORY CHAIN; TRANSPOSON;

BACTERIA (MICROORGANISMS); DESULFITOBACTERIUM DEHALOGENANS; ENTEROCOCCUS FAECALIS; POSIBACTERIA; PROKARYOTA;

EID: 0032718713     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.22.6882-6888.1999     Document Type: Article

References (25)

1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. H. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
2. Andrews, S. C., B. C. Berks, J. McClay, A. Ambler, M. A. Quail, P. Golby, and J. R. Guest. 1997. A 12 cistron Escherichia coli operon (hyf) encoding a putative proton translocating formate hydrogenlyase system. Microbiology 143:3633-3647.
3. Berks, B. C. 1996. A common export pathway for proteins binding complex redox cofactors? Mol. Microbiol. 22:393-404.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Clewell, D. B., S. E. Flannagan, and D. D. Jaworski. 1995. Unconstrained bacterial promiscuity: The Tn916-Tn1545 family of conjugative transposons. Trends Microbiol. 3:225-236.
6. de Vos, W. M., M. Kleerebezem, and O. P. Kuipers. 1997. Expression systems for industrial Gram-positive bacteria with low guanine and cytosine content. Curr. Opin. Biotechnol. 8:547-553.
7. El Fantroussi, S., H. Maveau, and S. N. Agathos. 1998. Anaerobic dechlorinating bacteria. Biotechnol. Prog. 14:167-188.
8. Fetzner, S. 1998. Bacterial dehalogenation. Appl. Microbiol. Biotechnol. 50:633-657.
9. Flannagan, S. E., L. A. Zitzow, Y. A. Su, and D. B. Clewell. 1994. Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis. Plasmid 32:350-354.
10. Gawron-Burke, C., and D. B. Clewell. 1984. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia colt: strategy for targeting and cloning of genes from gram-positive bacteria. J. Bacteriol. 159:214-221.
11. Gribble, G. W. 1996. Naturally occurring organohalogen compounds - a comprehensive survey. Fortschr. Chem. Org. Naturst. 68:1-423.
12. Hileman, B. 1993. Concerns broaden over chlorine and chlorinated hydrocarbons. Chem. Eng. News. 71:11-20.
13. Hill, C., G. Venema, C. Daly, and G. F. Fitzgerald. 1988. Cloning and characterization of the tetracycline resistance determinant of and several promoters from within the conjugative transposon Tn919. Appl. Environ. Microbiol. 54:1230-1236.
14. Holliger, C., G. Wohlfarth, and G. Diekert. 1998. Reductive dechlorination in the energy metabolism of anaerobic bacteria. FEMS Microbiol. Rev. 22:383-398.
15. Jacob, A. E., and S. J. Hobbs. 1974. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J. Bacteriol. 117:360-372.
16. Mohn, W. W., and J. M. Tiedje. 1992. Microbial reductive dehalogenation. Microbiol. Rev. 56:482-507.
17. Neumann, A., G. Wohlfarth, and G. Diekert. 1998. Tetrachloroethene dehalogenase from Dehalospirillum multivorans: cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli. J. Bacteriol. 180:4140-4145.
18. Renault, P., J. F. Nogrette, N. Galleron, J. J. Godon, and S. D. Ehrlich. 1995. Specificity of insertion of Tn1545 transposon family in Lactococcus lactis subsp. lactis. Dev. Biol. Stand. 85:535-541.
19. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
20. Sawers, G. 1994. The hydrogenases and formate dehydrogenases of Escherichia coli. Antonie Leeuwenhoek 66:57-88.
21. Scott, J. R., F. Bringel, D. Marra, G. Van Alstine, and C. K. Rudy. 1994. Conjugative transposition of Tn916: preferred targets and evidence for conjugative transfer of a single strand and for a double-stranded circular intermediate. Mol. Microbiol. 11:1099-1108.
22. Triglia, T., M. G. Peterson, and D. J. Kemp. 1988. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16:8186.
23. Utkim, I., D. D. Dalton, and J. Wiegel. 1995. Specificity of reductive dehalogenation of substituted ortho-chlorophenols by Desulfitobaclerium dehalogenans JW/IU-DC1. Appl. Environ. Microbiol. 61:346-351.
24. Utkin, I., C. Woese, and J. Wiegel. 1994. Isolation and characterization of Desulfitobacterium dehalogenans gen. nov., sp. nov., an anaerobic bacterium which reductively dechlorinates chlorophenolic compounds. Int. J. Syst. Bacteriol. 44:612-619.
25. van de Pas, B. A., H. Smidt, W. R. Hagen, J. van der Oost, G. Schraa, A. J. M. Stams, and W. M. de Vos. 1999. Purification and molecular characterization of ortho-chlorophenol reductive dehalogenase, a key enzyme of halorespiration in Desulfitobacterium dehalogenans. J. Biol. Chem. 274:20287-20292.