Comparison between McCoy cell line and HeLa cell line for detecting Helicobacter pylori cytotoxicity: Clinical and pathological relevance

Italian Journal of Gastroenterology and Hepatology

Volumn 31, Issue 8, 1999, Pages 651-658

  Hua Xiang Xia, H ^a   Gallagher, C ^a   Hyde, D ^a   Talley, N J ^a   Keane, C T ^a   O'Morain, C A ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Cytotoxicity; Gastroduodenal diseases; Helicobacter pylori; Mccoy cells

Indexed keywords

ARTICLE; CELL CULTURE; CELL LINE; CELL SUSPENSION; CELL VACUOLE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; DIGESTIVE TRACT ENDOSCOPY; DISEASE ACTIVITY; DISEASE SEVERITY; DYSPEPSIA; GASTRITIS; HELA CELL; HELICOBACTER PYLORI; HISTOLOGY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MICROSCOPY; NONHUMAN; PEPTIC ULCER; STOMACH MUCOSA;

ADOLESCENT; ADULT; AGED; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BIOLOGICAL ASSAY; CELL LINE; CYTOTOXINS; DYSPEPSIA; FEMALE; GASTRIC MUCOSA; GASTRITIS; HELA CELLS; HELICOBACTER PYLORI; HUMANS; MALE; MICE; MIDDLE AGED; PEPTIC ULCER;

EID: 0032710678     PISSN: 11258055     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Leunk RD, Johnson PT, David BC, Kraft WG, Morgan DR. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol 1988;26:93-9.
2. Figura N, Guglielmetti P, Rossolini A, Barberi A, Cusi G, Musmanno RA, et al. Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only. J Clin Microbiol 1989;27:225-6.
3. Cover TL, Dooley CP, Blaser MJ. Characterization of human sérologie response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect Immun 1990;58:603-10.
4. Fox JG, Correa P, Taylor NS, Thompson N, Fontham E, Janney F, et al. High prevalence and persistence of cytotoxin-positive Helicobacter pylori strains in a population with high prevalence of antral gastritis. Am J Gastroenterol 1992;87:1554-60.
5. Cover TL, Cao P, Lind CD, Tham KT, Blaser MJ. Correlation between vacuolating production by Helicobacter pylori isolates in vitro and in vivo. Infect Immun 1993;6:5008-12.
6. Xiang Z, Censini S, Bayeli PF, Telford JL, Figura N, Rappuoli R, et al. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that cagA is not necessary for expression of the vacuolating cytotoxin. Infect Immun 1995;63:94-8.
7. Tee W, Lambert JR, Dwyer B. Cytotoxin production by Helicobacter pylori from patients with upper gastrointestinal tract diseases. J Clin Microbiol 1995;33:1203-5.
8. Rudi J, Kolb C, Maiwald M, Zuna I, von Herbay A, Galle PR, et - al. Serum antibodies against Helicobacter pylori proteins VacA and CagA are associated with increased risk for gastric adenocarcinoma. Dig Dis Sei 1997;42:1652-9.
9. Leunk RD, Ferguson MA, Morgan DR, Low DE, Simor AE. Antibody to cytotoxin in infection by Helicobacter pylori. J Clin Microbiol 1990;28:1181-4.
10. Yamaoka Y, Kodama T, Graham DY, Kashima K. Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains. J Clin Microbiol 1998;36:3433-4.
11. Xiang Z, Bugnoli M, Ponzetto A, Morgando A, Figura N, Covacci A, et al. Detection of an enzyme immunoassay of an immune response to a recombinant fragment of the 128 kilodalton protein (CagA) of Helicobacter pylori. Eur J Clin Microbiol Infect Dis 1993;12:739-45.
12. n Cover TL, Glupczynski Y, Lage AP, Burette A, Tummuru MKR, Perez-Perez GI, et al. Serologie detection of infection with cagA+ Helicobacter pylori strains. J Clin Micro 1995:33:1496-500.
13. Weel JF, van der Hülst RW, Gerrits Y, Roorda P, Feller M, Dankert J, et al. The interrelationship between cytotoxin-associated gene A, vacuolating cytotoxin, and Helicobacter pylori-related diseases. J Infect Dis 1996;173:1171-5.
14. Owen RJ, Hurtado A, Banatvala N, Abdi Y, Davies GR, Feldman R, et al. Conservation of the cytotoxin-associated (cagA) gene of Helicobacter pylori and investigation of association with vacuolating-cytotoxin activity and gastroduodenal disease. FEMS Immunol Med Microbiol 1994:9:307-15.
15. Figura N, Armellini D, Bugnoli M, Bayeli PF, Gennari C, Crabtree JE. Activity of omeprazole on Helicobacter pylori and relation to toxicity of strains. J Clin Pathol 1994:47:440-2.
16. Pagliaccia C, De Bernard M, Lupetti P, Ji X, Burroni D, Cover TL, et al. The m2 form of the Helicobacter pylori cytotoxin has cell type-specific vacuolating activity. Proc Natl Acad Sei USA 1998:95:10212-7.
17. Schue V, Green GA, Monteil H. Comparison of the ToxA test with cytotoxicity assay and culture for the detection of Clostridium difficile-associated diarrhoea disease. J Med Microbiol 1994;41:316-8.
18. Talley AR, Garcia-Ferrer F, Laycock KA, Essary LR, Holcomb WL, Floweras BE, et al. Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture. Am J Ophthalmol 1994;! 17:50-7.
19. Dixon MF, Genta RM, Yardley JH, Correa P, and the participants in the International Workshop on the Histopathology of Gastritis, Houston 1994. Classification and grading of gastritis. The updated Sydney System. Am J Surg Pathol 1996:20:1161-81.
20. Kamiya S, Kai M, Ozawa A, Kobayashi H, Kobayashi H, Shirai T, et al. Characteristics of vacuolating toxin produced by Helicobacter pylori. Eur J Gastroenterol Hepatol 1994;6(Suppl. l):S23-7.
21. Cover TL, Blaser MJ. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J Biol Chem 1992:267:10570-5.
22. Atherton JC, Cao P, Peek Jr RM, Tummuru MKR, Blaser MJ, Cover TL. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J Biol Chem 1995; 270:17771-7.
23. Atherton JC, Peek RM, Tham KT, Cover TL, Blaser MJ. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 1997:112:92-9.
24. Go MF, Cissell L, Graham DY. Failure to confirm association of vac A gene mosaicism with duodenal ulcer disease. Scand J Gastroenterol 1998:33:132-6.
25. Figura N, Vindigni C, Present! L, Carducci A. New acquisitions in Helicobacter pylori characteristics. Ital J Gastroenterol Hepatol 1998;30(Suppl. 3):S254-8.
26. Cover TL, Cao P, Murthy UK, Sipple MS, Blaser MJ. Serum neu-tralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori. J Clin Invest 1992;90:913-8.
27. Crabtree JE, Taylor JD, Wyatt JI, Heatley RV, Shallcross TM, Tompkins DS, et al. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulcération, and gastric pathology. Lancet 1991;338:332-5.
28. Crabtree JE, Farmery SM, Lindley IJD, Figura N, Peichl P, Tompkins DS. CagA/cytotoxic strains of Helicobacter pylori and interleukin-8 in gastric epithelial cell lines. J Clin Pathol 1994;47:945-50.
29. Zhang QB, Nakashabendi IM, Mokhashi MS, Dawodu JB, Gemmell CG, Russell RI. Association of cytotoxin production and neutrophil activation by strains of Helicobacter pylori isolated from patients with peptic ulcération and chronic gastritis. Gut 1996;38:841-5.