Sterols of Pneumocystis carinii hominis organisms isolated from human lungs

Clinical and Diagnostic Laboratory Immunology

Volumn 6, Issue 6, 1999, Pages 970-976

  Kaneshiro, Edna S ^a   Amit, Zunika ^a   Chandra, Jyotsna ^a   Baughman, Robert P ^b   Contini, Carlo ^c   Lundgren, Bettina ^d  

^a UNIVERSITY OF CINCINNATI   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

^d STATENS SERUM INSTITUT   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL; STEROL; STEROL DERIVATIVE;

ARTICLE; BACTERIUM ISOLATION; CONTROLLED STUDY; GAS CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; LUNG LAVAGE; NONHUMAN; PNEUMOCYSTIS CARINII; PRIORITY JOURNAL; STEROL ANALYSIS; STEROL SYNTHESIS;

EID: 0032707494     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.6.6.970-976.1999     Document Type: Article

References (31)

1. Amit, Z., R. P. Baughman, and E. S. Kaneshiro. Pneumocystis carinii sterols in human bronchoalveolar lavage fluid. Submitted for publication.
2. Banerji, S., E. B. Lugli, and A. E. Wakefield. 1994. Identification of two genetically distinct strains of Pneumocystis carinii in infected ferret lungs. J. Eukaryot. Microbiol. 41:73S.
3. Bartlett, M. S., R. Eichboltz, and J. W. Smith. 1985. Antimicrobial susceptibility of Pneumocystis carinii in culture. Diagn. Microbiol. Infect. Dis. 3:381-387.
4. Baughman, R. P., M. N. Dohn, and P. T. Frame. 1994. The continuing utility of bronchoalveolar lavage to diagnose opportunistic infection in AIDS patients. Am. J. Med. 97:515-522.
5. Baughman, R. P., M. N. Dohn, R. G. Loudon, and P. T. Frame. 1991. Bronchoscopy with bronchoalveolar lavage in tuberculosis and fungal infections. Chest 99:92-97.
6. Baughman, R. P., S. Strohofer, G. Colangelo, and P. T. Frame. 1990. Semiquantitative technique for estimating Pneumocystis carinii burden in the lung. J. Clin. Microbiol. 28:1425-1427.
7. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37:911-917.
8. Chandra, J., Z. Amit, R. P. Baughman, B. Kleykamp, and E. S. Kaneshiro. 1999. Pneumocystis infection is correlated with a reduction of the total sterol content of human bronchoalveolar lavage fluid. J. Eukaryot. Microbiol. 46: 146S-148S.
9. Cushion, M. T. 1998. Pneumocystis carinii, p. 645-683. In L. Collier, A. Balows, and M. Sussman (ed.), Topley and Wilson's microbiology and microbial infections. Medical mycology, 9th ed., vol. 4. Arnold Publishers, Oxford University Press, New York, N.Y.
10. Cushion, M. T., J. Zhang, M. Kaselis, D. Giuntoli, S. L. Stringer, and J. R. Stringer. 1993. Evidence for two genetic variants of Pneumocystis carinii coinfecting laboratory rats. J. Clin. Microbiol. 31:1217-1223.
11. Florin-Christensen, M., J. Florin-Christensen, Y. P. Wu, L. Zhou, A. Gupta, H. Rudney, and E. S. Kaneshiro. 1994. Occurrence of specific sterols in Pneumocystis carinii. Biochem. Biophys. Res. Commun. 198:236-242.
12. Folch, J., M. Lees, and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226:497-509.
13. Furlong, S. T., J. A. Samia, R. M. Rose, and J. A. Fishman. 1994. Phytosterols are present in Pneumocystis carinii. Antimicrob. Agents Chemother. 38:2534-2540.
14. Hoffman, A. G. D., M. G. Lawrence, F. P. Ognibene, A. F. Suffredini, G. Y. Lipschik, J. A. Kovacs, H. Masur, and J. H. Shelhamer. 1992. Reduction of pulmonary surfactant in patients with human immunodeficiency virus infection and Pneumocystis carinii pneumonia. Chest 102:1730-1736.
15. Kaneshiro, E. S. 1998. The lipids of Pneumocystis carinii. Clin. Microbiol Rev. 11:27-41.
16. 15a. Kaneshiro, E. S., et al. Unpublished data.
17. Kaneshiro, E. S., Z. Amit, M. M. Swonger, G. P. Kreishman, E. E. Brooks, M. Kreishman, K. Jayasimhulu, E. J. Parish, H. Sun, S. A. Kizito, and D. H. Beach. 1999. Pneumocysterol [(24Z)-ethylidenelanost-8-en-3β-ol], a rare sterol detected in the opportunistic pathogen Pneumocystis carinii hominis: structural identity and chemical synthesis. Proc. Natl. Acad. Sci. USA 96: 97-102.
18. Kaneshiro, E. S., J. E. Ellis, K. Jayasimhulu, and D. H. Beach. 1994. Evidence for the presence of "metabolic sterols" in Pneumocystis: identification and initial characterization of Pneumocystis carinii sterols. J. Eukaryot. Microbiol. 41:78-85.
19. Kaneshiro, E. S., M. A. Wyder, L. H. Zhou, J. E. Ellis, D. R. Voelker, and S. G. Langreth. 1993. Characterization of Pneumocystis carinii preparations developed for lipid analysis. J. Eukaryot. Microbiol. 40:805-815.
20. Keely, S. P., J. R. Stringer, R. P. Baughman, M. J. Linke, P. D. Walzer, and A. G. Smulian. 1995. Genetic variation among Pneumocystis carinii hominis isolates in recurrent pneumocystosis. J. Infect. Dis. 172:595-598.
21. Kernbaum, S., U. Masliah, L. G. Alcindor, C. Bouton, and D. Cristol. 1983. Phospholipase activities of bronchoalveolar lavage fluid in rat Pneumocystis carinii pneumonia. Br. J. Exp. Pathol. 64:460-470.
22. Latouche, S., P. Roux, J. L. Poirot, I. Lavrard, B. Hermelin, and V. Bertrand. 1994. Preliminary results of Pneumocystis carinii strain differentiation by using molecular biology. J. Clin. Microbiol. 32:3052-3053.
23. Peglow, S. L., A. G. Smulian, M. J. Linke, C. L. Pogue, S. Nurre, J. Crisler, J. Phair, J. W. Gold, D. Armstrong, and P. D. Walzer. 1990. Serologic responses to Pneumocystis carinii antigens in health and disease. J. Infect. Dis. 161:296-306.
24. Pifer, L. L., W. T. Hughes, S. Stagno, and D. Woods. 1978. Pneumocystis carinii infection: evidence for high prevalence in normal and immunosuppressed children. Pediatrics 61:35-41.
25. Sarić, M., and A. B. Clarkson, Jr. 1994. Ornithine decarboxylase in Pneumocystis carinii and implications for therapy. Antimicrob. Agents Chemother. 38:2545-2552.
26. 2 in experimental Pneumocystis carinii pneumonia. Am. Rev. Respir. Dis. 134:526-531.
27. Stringer, J. R., and P. D. Walzer. 1996. Molecular biology and epidemiology of Pneumocystis carinii infection in AIDS. AIDS 10:561-571.
28. 24(25) sterol methyltransferase block sterol synthesis and cell proliferation in Pneumocystis carinii. Antimicrob. Agents Chemother. 41:1428-1432.
29. Vanhanen, H. T., and T. A. Miettinen. 1992. Effects of unsaturated and saturated dietary plant sterols on their serum contents. Clin. Chim. Acta 205:97-107.
30. Wakefield, A. E., T. J. Stewart, E. R. Moxon, K. Marsh, and J. M. Hopkin. 1990. Infection with Pneumocystis carinii is prevalent in healthy Gambian children. Trans. R. Soc. Trop. Med. Hyg. 84:800-802.
31. Walzer, P. D., M. E. Rutledge, and K. Yoneda. 1979. Pneumocystis carinii: new Separation method from lung tissue. Exp. Parasitol. 47:356-368.