Structural changes induced by catalytic turnover at the molybdenum site of Arabidopsis nitrate reductase [5]

Journal of the American Chemical Society

Volumn 121, Issue 41, 1999, Pages 9730-9731

  George, Graham N ^a   Mertens, Jeffrey A ^b   Campbell, Wilbur H ^b  

^a SLAC NATIONAL ACCELERATOR LABORATORY   (United States)

^b MICHIGAN TECHNOLOGICAL UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MOLYBDENUM; NITRATE REDUCTASE;

AMINO ACID SEQUENCE; ARABIDOPSIS; CATALYSIS; CONFORMATION; ENZYME ANALYSIS; ENZYME STRUCTURE; LETTER; NONHUMAN; REDUCTION;

ARABIDOPSIS;

EID: 0032704113     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja990310l     Document Type: Letter

References (28)

1. (a) Campbell, W. H. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1999, 50, 227-303.
2. (b) Campbell, W. H. Plant Physiol. 1996 111, 355-361.
3. Hille, R. Chem. Rev. 1996, 96, 2757-2816 and references therein.
4. (a) Bray, R. C. Q. Rev. Biophys. 1988, 21, 299-329 and references therein.
5. (b) Cramer. S. P.; Wahl, R.; Rajagopalan, K. V. J. Am. Chem. Soc. 1981, 103, 7721-7727.
6. (c) George, G. N.; Kipke, C. A.; Prince, R. C.; Sunde, R. A.; Enemark, J. H.; Cramer, S. P. Biochemistry 1989, 28, 5075-5080.
7. (d) Garton, S. D.; Garrett, R. M.; Rajagopalan, K. V.; Johnson, M. K. J. Am. Chem. Soc. 1997, 119, 2590-2591.
8. (e) Raimsimring, A. M.; Pacheo, A.; Enemark, J. H. J. Am. Chem. Soc. 1998, 120, 11263-11278.
9. George, G. N.; Garrett, R. M.; Prince, R. C.; Rajagopalan, K. V. J. Am. Chem. Soc. 1996, 118, 8588-8592.
10. George, G. N.; Garrett, R. M.; Graf, T.; Prince, R. C.; Rajagopalan, K. V. J. Am. Chem. Soc. 1998, 120, 4522-4523.
11. Kisker, C.; Schindelin, H.; Pacheco, A.; Wehbi, W. A.; Garrett, R. M.; Rajagopalan, K. V.; Enemark, J. E.; Rees, D. C. Cell 1997, 91, 973-983.
12. George, G. N.; Pickering, I. J.; Kisker, C. Inorg. Chem. 1999, 38, 2539-2540.
13. The assimilatory nitrate reductase from Chlorella vulgaris has previously been studied by EXAFS spectroscopy [Cramer, S. P.; Solomonson, L. P.; Adams, M. W. W.; Mortenson, L. E. J. Am. Chem. Soc. 1984, 106, 1467-1471]. This study indicated an oxidized active site possessing two Mo=O and between two and three Mo-S ligands, and a reduced active site with a single Mo=O, about three Mo-S ligands and probably one Mo-O ligand.
14. 12 No smoothing or related manipulation was performed upon any of the data.
15. Su W.; Mertens, J. A. ;Kanamaru, K.; Campbell, W. H.; Crawford, N. M. Plant Physiol. 1997, 115, 1135-1143.
16. http://ssrl.slac.stanford.edu/exafspak.html
17. (a) Rehr, J. J.; Mustre de Leon, J.; Zabinsky, S. I.; Albers, R. C. J. Am. Chem. Soc. 1991, 113, 5135-5140.
18. (b) Mustre de Leon, J.; Rehr, J. J.; Zabinsky, S. I.; Albers, R. C. Phys. Rev. 1991, B44, 4146-4156.
19. George, G. N. JBIC 1997, 2, 790-796.
20. George, G. N.; Hilton, J.; Temple, C.; Prince, R. C.; Rajagopalan, K. V. J. Am. Chem. Soc. 1999, 121, 1256-1266.
21. For a definition of the EXAFS error function used, and the curve-fitting methods employed see ref 4.
22. 8 indicates a significantly improved signal to noise in the present work. This can be attributed to advances in synchrotron radiation sources, modern detector technology [Cramer, S. P.; Tench, O.; Yocum, M.; George, G. N. Nucl. Instrum. Methods Phys. Res. 1988, A266, 586-591]. and the availability of stable cryostat systems.
23. Das, S. K.; Chaudhury, P. K.; Biswas, D.; Sarkar, S. J. Am. Chem. Soc. 1994, 116, 9061-9070.
24. Schultz, B. E.; Gheller, S. F.; Mutterties, M. C.; Scott, M. J.; Holm, R. H. J. Am. Chem. Soc. 1993, 115, 2714-2722.
25. Oku, H.; Ueyama, N.; Nakamura, A. Inorg. Chem. 1995, 34, 3667-3676.
26. 6 suggest that a square-based pyramidal site of the type suggested for oxidized sulfite oxidase could show quite small distortion of Mo-S bond lengths from Mo=O trans effects, which might not be detected by EXAFS (i.e. <0.1 A°).
27. Solomonson, L. P.; Barber, M. J.; Howard, W. D.; Johnson J. L.; Rajagopalan, K. V. J. Biol. Chem. 1984. 259, 849-853.
28. Stiefel, E. I. In Comprehensive Coordination Chemistry; Wilkinson, G., Ed.; Pergamon Press: Oxford, 1987 pp 1375-1420.