Drug metabolizing capacity of cryopreserved human, rat, and mouse liver parenchymal cells in suspension

Drug Metabolism and Disposition

Volumn 27, Issue 12, 1999, Pages 1415-1422

  Steinberg, Pablo ^a,b   Fischer, Thomas ^a   Kiulies, Sandra ^a   Biefang, Katja ^a   Platt, Karl Ludwig ^a   Oesch, Franz ^a   Böttger, Thomas ^a   Bulitta, Clemens ^a   Kempf, Peter ^a,c   Hengstler, Jan ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b UNIVERSITY OF POTSDAM   (Germany)

^c Chirurgische Abteilung   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

7 ETHOXYCOUMARIN DEETHYLASE; CYTOCHROME P450; ETHOXYRESORUFIN DEETHYLASE; GLUCURONOSYLTRANSFERASE; GLUTATHIONE TRANSFERASE; HYMECROMONE; LACTATE DEHYDROGENASE; PHENOBARBITAL; TESTOSTERONE DERIVATIVE;

ANIMAL CELL; ARTICLE; CELL SUSPENSION; CRYOPRESERVATION; DRUG METABOLISM; ENZYME ACTIVITY; HUMAN; HUMAN CELL; LIVER CELL; LIVER MICROSOME; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; RAT; THAWING;

ANIMALS; BIPHENYL COMPOUNDS; CRYOPRESERVATION; GLUCURONOSYLTRANSFERASE; GLUTATHIONE TRANSFERASE; HUMANS; HYMECROMONE; LIVER; MALE; MICE; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0032698251     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Arand M, Robertson LW and Oesch F (1987) Fluorimetric assay for quantitating phenol sulfotransferase activities in homogenates of cells and tissues. Anal Biochem 163:546-551.
2. Bayliss MK, Bell JA, Wilson K and Park GR (1994) 7-Ethoxycoumarin O-deethylase kinetics in rat, dog and human hepatocyte suspension. Xenobiotica 24:231-241.
3. Bock KW, Burchell B, Dutton GJ, Hanninen O, Owens IS, Siest G and Tephly TR (1983) UDP-glucuronosyltransferases. Guidelines for consistent interim terminology and assay conditions. Biochem Pharmacol 32:953-955.
4. Bock KW, von Clausbruch UC, Kaufmann R, Lilienblum W, Oesch F, Pfeil H and Platt KL (1980) Functional heterogeneity of UDP-glucuronosyltransferase in rat tissues. Biochem Pharmacol 29:495-500.
5. Bradford MM (1969) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 27:502-522.
6. Burke MD and Mayer RT (1974) Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab Dispos 2:583-588.
7. Diener B, Abdel-Latif H, Arand M and Oesch F (1995) Xenobiotic metabolizing enzyme activities and viability are well preserved in EDTA-isolated rat liver parenchymal cells after cryopreservation. Toxicol Appl Pharmacol 130:149-153.
8. Diener B, Traiser M, Arand M, Leissner J, Witsch U, Hohenfellner R, Fändrich F, Vogel I, Utesch D and Oesch F (1994) Xenobiotic metabolizing enzyme activities in isolated and cryopreserved human liver parenchymal cells. Toxicol In Vitro 8:1161-1166.
9. Diener B, Utesch D, Beer N, Dürk H and Oesch F (1993) A method for the cryopreservation of liver parenchymal cells for studies of xenobiotics. Cryobiology 30:116-127.
10. Habig WH, Pabst MJ and Jakoby WB (1974) Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130-7139.
11. Jackson BA, Davies JE and Chipman JK (1985) Cytochrome P450 activity in hepatocytes following cryopreservation and monolayer culture. Biochem Pharmacol 34:3389-3391.
12. Kern A, Bader A, Pilchmayr R and Sewing K-F (1997) Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochem Pharmacol 54:761-772.
13. Kimonen T, Juvonen RO, Alhava E and Pasanen M (1995) The inhibition of CYP enzymes in mouse and human liver by pilocarpine. Br J Pharmacol 114:832-836.
14. Lake BG (1987) Preparation and characterisation of microsomal fractions for studies of xenobiotic metabolism, in Biochemical Toxicology (Snell K and Mullock B eds) pp 183-215, IRL Press, Oxford.
15. Loretz LJ, Li AP, Flye MW and Wilson AGE (1989) Optimization of cryopreservation procedures for rat and human hepatocytes. Xenobiotica 19:489-498.
16. Lubet RA, Nims RW, Mayer RT, Cameron JW and Schechtman LM (1985) Measurement of cytochrome P-450 dependent alkylation of alkoxyphenazones in hepatic S9s and hepatocyte homogenates: Effects of dicumarol. Mutat Res 142:127-131.
17. Mäenpää J, Syngelma T, Honkakoski P, Lang MA and Pelkonen O (1991) Comparative studies on coumarin and testosterone metabolism in mouse and human livers: Differential inhibitions by the anti-P450Coh antibody and metyrapone. Biochem Pharmacol 42:1229-1235.
18. Moldéus P, Högberg J and Orrenius S (1978) Isolation and use of liver cells, in Methods in Enzymology (Fleischer S and Packer L eds) pp 60-71, Academic Press, New York.
19. Novicki DL, Irons GP, Strom SC, Jirtle R and Michalopoulos G (1982) Cryopreservation of isolated rat hepatocytes. In Vitro 18:393-399.
20. Oesch F, Wagner H, Platt KL and Arand M (1992) Improved sample preparation for the testosterone hydroxylation assay using disposable extraction columns. J Chromatogr 582: 232-235.
21. Seglen P (1976) Preparation of isolated rat liver cells, in Methods in Cell Biology (Prescott DM ed) vol 13, pp 29-83, Academic Press, New York.
22. Skett P (1994) Problems in using isolated and cultured hepatocytes for xenobiotic metabolism/ metabolism-based toxicity testing-solutions? Toxicol In Vitro 8:491-504.
23. Steinberg P, Lafranconi WM, Wolf CR, Waxman DJ, Oesch F and Friedberg T (1987) Xenobiotic metabolizing enzymes are not restricted to parenchymal cells in rat liver. Mol Pharmacol 32:463-470.
24. Swales NJ and Utesch D (1998) Metabolic activity of fresh and cryopreserved dog hepatocyte suspensions. Xenobiotica 28:937-948.
25. Utesch D, Diener B, Molitor E, Oesch F and Plan KL (1992) Characterization of cryopreserved rat liver parenchymal cells by metabolism of diagnostic substrates and activities of related enzymes. Biochem Pharmacol 44:309-315.
26. Utesch D, Glatt HR and Oesch F (1987) Rat hepatocyte-mediated bacterial mutagenicity in relation to the carcinogenic potency of benzo[a]anthracene, benzo[a]pyrene, and twenty five methylated derivatives. Cancer Res 47:1509-1515.
27. Waxman DJ, Ko A and Walsh C (1983) Regioselectivity and stereoselectivity of androgen hydroxylations catalyzed by cytochrome P-450 isozymes purified from phenobarbital-induced rat liver. J Biol Chem 258:11937-11947.
28. Weaver RJ, Thompson S, Smith G, Dickins M, Elcombe CR, Mayer RT and Burke MD (1994) A comparative study of constitutive and induced alkoxyresorufin O-dealkylation and individual cytochrome P-450 forms in cynomolgus mokey (Macaca fascicularis), human, mouse, rat and hamster liver microsomes. Biochem Pharmacol 47:763-773.
29. Wood AW, Ryan DE, Thomas PE and Levin W (1983) Regio- and stereoselective metabolism of two C19 steroids by five highly purified and reconstituted rat hepatic cytochrome P-450 isozymes. J Biol Chem 258:8839-8847.