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Volumn 286, Issue 5443, 1999, Pages 1365-1367

Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90(Rsk)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOSTATIC AGENT; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE;

EID: 0032698220     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5443.1365     Document Type: Article
Times cited : (140)

References (29)
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    • 32P]ATP, and 250 μM S6 peptide (Upstate Biotechnology, Waltham, MA). Where indicated in Fig. 2, assays also contained 10 units of MAPK (New England Biolabs, Beverly, MA) or 2.5 ng of activated PDK-1 (Upstate Biotechnology). Reactions were incubated at 30°C for 60 min and quenched by the addition of EDTA to 60 mM. The beads were removed by centrifugation at 16,000g, and 10 μl of the supernatant were spotted onto p81 paper in duplicate, washed, dried, and counted by the Cerenkov method. Protein immunoblots were performed essentially as described elsewhere (3, 4).
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    • xe antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and antibody to cydin B2 were used as previously described (3).
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    • note
    • Rsk (9) and E. Erikson for a critical reading of the manuscript. Supported by grant DK28353 from NIH. S.D.G. and M.S.S. are Associates and J.LM. is an Investigator of the Howard Hughes Medical Institute.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.