High-Performance Liquid Chromatographic Analysis of Chlorhexidine in Saliva after Mouthrinsing

Caries Research

Volumn 33, Issue 2, 1999, Pages 156-163

  Miyazaki, T ^a   Ohmoto, S ^a   Tsuchiya, Hironori ^a  

^a ASAHI UNIVERSITY   (Japan)

Author keywords

Chlorhexidine; High performance liquid chromatography; Quantitative analysis; Retention; Saliva

Indexed keywords

CHLORHEXIDINE; HYDROXYAPATITE; TOPICAL ANTIINFECTIVE AGENT;

ADSORPTION; ARTICLE; CHEMISTRY; EPITHELIUM CELL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MALE; MIDDLE AGED; MOUTH HYGIENE; PROTEIN BINDING; SALIVA;

ADSORPTION; ANTI-INFECTIVE AGENTS, LOCAL; CHLORHEXIDINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DURAPATITE; EPITHELIAL CELLS; HUMANS; MALE; MIDDLE AGED; MOUTHWASHES; PROTEIN BINDING; SALIVA;

EID: 0032615794     PISSN: 00086568     EISSN: None     Source Type: Journal    
DOI: 10.1159/000016510     Document Type: Article

References (20)

1. Bonesvoll P, Gjermo P: A comparison between chlorhexidine and some quaternary ammonium compounds with regard to retention, salivary concentration and plaque-inhibiting effect in the human mouth after mouth rinses. Arch Oral Biol 1978;23:289-294.
2. Bonesvoll P, Lökken P, Rølla G, Paus PN: Retention of chlorhexidine in the human oral cavity after mouth rinses. Arch Oral Biol 1974;19: 209-212.
3. Cohen RE, Levine MJ: Salivary glycoproteins; in Tenovuo JO (ed): Human Saliva: Clinical Chemistry and Microbiology. Boca Raton, CRC Press, 1989, vol 1, pp 101-130.
4. Gjermo P: Chlorhexidine and related compounds. J Dent Res 1989;68(special issue):1602-1608.
5. Huizinga ED, Ruben J, Arends J: The effect of an antimicrobial-containing varnish on root demineralization in situ. Caries Res 1990;24:130-132.
6. Jenkins S, Addy M, Wade W: The mechanism of action of chlorhexidine: A study of plaque growth on enamel inserts in vivo. J Clin Periodontol 1988;15:415-424.
7. Jensen JE, Christensen F: A study of the elimination of chlorhexidine from the oral cavity using a new spectrophotometric method. J Periodontal Res 1971;6:306-311.
8. Katz S, Park KK, Stookey GK, Schemehorn BR: Development and initial testing of a model for in vitro formation of pit and fissure caries. Caries Res 1986;20:424-428.
9. Lam YWF, Chan DCN, Rodriguez SY, Lintakoon JH, Lain T-H: Sensitive high-performance liquid chromatographic assay for the determination of chlorhexidine in saliva. J Chromatogr 1993;612:166-171.
10. Levine MJ: Salivary macromolecules; in Malamud D, Tabak L (eds): Saliva as a Diagnostic Fluid. New York, New York Academy of Sciences, 1993, pp 11-16.
11. Malamud D, Tabak L: Guidelines for saliva nomenclature and collection; in Malamud D, Tabak L (eds): Saliva as a Diagnostic Fluid. New York, New York Academy of Sciences, 1993, pp xi-xii.
12. Pesonen T, Holmalahti J, Pohjola J: Determination of chlorhexidine in saliva using high-performance liquid chromatography. J Chromatogr B 1995;665:222-225.
13. Sebille B, Zini R, Madjar C-V, Thuaud N, Tillement J-P: Separation procedures used to reveal and follow drug-protein binding. J Chromatogr 1990;531:51-77.
14. Slomiany BL, Murty VL, Piotrowski J, Slomiany A: Salivary mucins in oral mucosal defense. Gen Pharmacol 1996;27:761-771.
15. Steinberg D, Kopec LK, Bowen WH: Adhesion of Actinomyces isolates to experimental pellicles. J Dent Res 1993;72:1015-1020.
16. Tsuchiya H, Sato M, Iinuma M, Yokoyama J, Ohyama M, Tanaka T, Takase I, Namikawa I: Inhibition of the growth of cariogenic bacteria in vitro by plant flavanones. Experientia 1994; 50:846-849.
17. Tsuchiya H, Sato M, Kato H, Okubo T, Juneja LR, Kim M: Simultaneous determination of catechins in human saliva by high-performance liquid chromatography. J Chromatogr B 1997; 703:253-258.
18. de Vries J, Ruben J, Arends J: Determination of chlorhexidine in saliva and in aqueous solutions. Caries Res 1991;25:410-414.
19. Walton JG, Thompson JW, Seymour RA: Textbook of Dental Pharmacology and Therapeutics, ed 2. Oxford, Oxford University Press, 1994, pp 147-149.
20. Webb BC, Willcox MDP, Thomas CJ, Harty DWS, Knox KW: The effect of sodium hypochlorite on potential pathogenic traits of Candida albicans and other Candida species. Oral Microbiol Immunol 1995;10:334-341.