Class II-lndependent generation of CD4 memory T cells from effectors

Science

Volumn 286, Issue 5443, 1999, Pages 1381-1383

  Swain, Susan L ^a   Hu, Hui ^a   Huston, Gail ^a  

^a TRUDEAU INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CD4 ANTIGEN; GAMMA INTERFERON; INTERLEUKIN 2; INTERLEUKIN 4; INTERLEUKIN 5; CYTOKINE; HLA ANTIGEN CLASS 2; LYMPHOCYTE ANTIGEN RECEPTOR;

ANIMAL CELL; ARTICLE; CELL SURVIVAL; CYTOKINE PRODUCTION; EFFECTOR CELL; HELPER CELL; LYMPHOCYTE PROLIFERATION; MAJOR HISTOCOMPATIBILITY COMPLEX; MEMORY CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE SUBPOPULATION; ADOPTIVE TRANSFER; ANIMAL; BIOSYNTHESIS; C57BL MOUSE; CELL DIVISION; CYTOLOGY; IMMUNIZATION; IMMUNOLOGICAL MEMORY; IMMUNOLOGY; MOUSE MUTANT; TH1 CELL; TH2 CELL; TRANSGENIC MOUSE;

ADOPTIVE TRANSFER; ANIMALS; CELL DIVISION; CYTOKINES; HISTOCOMPATIBILITY ANTIGENS CLASS II; IMMUNIZATION; IMMUNOLOGIC MEMORY; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICE, TRANSGENIC; RECEPTORS, ANTIGEN, T-CELL; T-LYMPHOCYTE SUBSETS; TH1 CELLS; TH2 CELLS;

EID: 0032589687     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5443.1381     Document Type: Article

References (29)

1. P. R. Rogers, G. Huston, S. L. Swain, J. Immunol. 161, 3844 (1998); C. Dubey, M. Croft, S. L. Swain, J. Immunol. 155, 45 (1995).
2. P. R. Rogers, G. Huston, S. L. Swain, J. Immunol. 161, 3844 (1998); C. Dubey, M. Croft, S. L. Swain, J. Immunol. 155, 45 (1995).
3. S. L. Swain, Immunity 1, 543 (1994).
4. D. Gray and P. Matzinger, J. Exp. Med. 174, 969 (1991).
5. L. L. Lau, B. D. Jamieson, T. Somasundaram, R. Ahmed, Nature 369, 648 (1994).
6. K. A. Hogquist et al., Cell 76, 17 (1994).
7. C. F. Tanchot, A. Lemonnier, B. Perarnau, A. A. Freitas, B. Rocha, Science 276, 2057 (1997).
8. S. Takeda, H.-R. Rodewald, H. Arakawa, H. Bluethman, T. Shimuzu, Immunity 5, 217 (1996); J. Kirberg, A. Berns, H. von Boehmer, J. Exp. Med. 186, 1269 (1997); T. Brocker, J. Exp. Med. 186, 1223 (1997).
9. S. Takeda, H.-R. Rodewald, H. Arakawa, H. Bluethman, T. Shimuzu, Immunity 5, 217 (1996); J. Kirberg, A. Berns, H. von Boehmer, J. Exp. Med. 186, 1269 (1997); T. Brocker, J. Exp. Med. 186, 1223 (1997).
10. S. Takeda, H.-R. Rodewald, H. Arakawa, H. Bluethman, T. Shimuzu, Immunity 5, 217 (1996); J. Kirberg, A. Berns, H. von Boehmer, J. Exp. Med. 186, 1269 (1997); T. Brocker, J. Exp. Med. 186, 1223 (1997).
11. R. Rooke, C. Waltzinger, C. Benoist, D. Mathis, Immunity 7, 123 (1997).
12. C. Dubey, M. Croft, S. L. Swain, J. Immunol. 157, 3280 (1996).
13. D. Nesic and S. Vukmanovic, J. Immunol. 160, 3705 (1998); M. A. Markiewicz et al., Proc. Natl. Acad. Sci. U.S.A. 95, 3065 (1998).
14. D. Nesic and S. Vukmanovic, J. Immunol. 160, 3705 (1998); M. A. Markiewicz et al., Proc. Natl. Acad. Sci. U.S.A. 95, 3065 (1998).
15. J. Kaye et al., Nature 341, 746 (1989).
16. X. Zhang et al., J. Exp. Med. 185, 1837 (1997).
17. P.-J. Linton, L. Haynes, N. R. Klinman, S. L. Swain, J. Exp. Med. 184, 1891 (1996).
18. + cells were detected in the effector population, but generation of memory was retained (Web table 1). The effector populations responded vigorously to peptide in the presence of freshly added APCs, but addition of peptide to the effector population without fresh APCs resulted in no cytokine production [Web table 2 (25)], further supporting the lack of any functional APCs in the effector population.
19. Y. Shinkai et al., Science 259, 822 (1993).
20. 7 effectors per mouse by intravenous injection. For analysis, recipients were killed at different times and their spleens and lymph node lymphocytes were analyzed by flow cytometry and by their capacity for cytokine production. For flow cytometry, recovered cells were stained with fluorescein isothiocyanate (FITC)-Vα11, phycoerythrin (PE)-Vβ3, cychrome-CD4, and strepavidin-APC-Thy1.1. The majority of cells that were positive for CD4 and for Thy1.1 donor marker were also positive for both chains of Tg TCR. Cells staining for all four markers were considered "donor cells." Donor cell recovery was the product of the fraction of donor cells in each population and in total cell recovery. The mean of results from individual animals and the standard error of that mean are shown in Fig. 1, B and C, whereas values from several individual mice are shown in Figs. 1A, 2A, 3A, and 3B.
21. -/- mice (class II KO) deficient in the β chain of class II on a B6 background. In transfers to such hosts we have never seen any evidence of either host-versus-graft or graft-versus-host response in more than 40 transfers for ATXBM and 10 transfers for class II KO recipients.
22. + cells.
23. B. Rocha, N. Dautigny, P. Pereira, Eur. J. Immunol. 19, 905 (1989).
24. Naïve CD4 T cells from AND mice were labeled with carboxyfluorescein succinimidyl ester and transferred into ATXBM and class II KO hosts. The naïve cells had a short half-life in the dass II KO hosts (11 days) compared to the ATXBM host (>35 days), consistent with a role of dass II in prolonging naïve CD4 life span (H. Hu, J. Decker, G. Huston, S. Swain, unpublished data).
25. Experiments of identical design to those described in Figs. 1C and 2 have been done using Th1 effectors with equivalent results. Memory cells developed and persisted in both ATXBM and dass II KO hosts at similar levels (S. Swain, unpublished data).
26. D. F. Tough and J. Sprent, J. Exp. Med. 179, 1127 (1994).
27. K. Murali-Krishna et al., Science 286, 1377 (1999).
28. H. Hu and S. Swain, data not shown.
29. Supplemental material may be found at www. sciencemag.org/feature/data/1042092.shl