Thymosin-α1 stimulates maturation of CD34+ stem cells into CD3+4+ cells in an in vitro thymic epithelia organ coculture model

International Journal of Immunopharmacology

Volumn 21, Issue 1, 1999, Pages 15-26

  Knutsen, Alan P ^a,b   Freeman, John J ^a   Mueller, Kathleen R ^a   Roodman, Stanford T ^a   Bouhasin, John D ^a  

^a SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b SAINT LOUIS UNIVERSITY   (United States)

Author keywords

CD34+ stem cells; Cultured thymic epithelia organ culture; Thymopoiesis; Thymosin 1

Indexed keywords

CD34 ANTIGEN; THYMOSIN; CD3 ANTIGEN; DRUG DERIVATIVE; IMMUNOLOGICAL ADJUVANT; INTERLEUKIN 7; THYMOSIN ALPHA1;

ARTICLE; CELL MATURATION; COCULTURE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IN VITRO STUDY; ORGAN CULTURE; PRIORITY JOURNAL; STEM CELL; T LYMPHOCYTE SUBPOPULATION; THYMOCYTE; THYMUS; BIOSYNTHESIS; CD4+ T LYMPHOCYTE; CELL DIVISION; CONFOCAL MICROSCOPY; CYTOLOGY; DRUG EFFECT; EPITHELIUM CELL; FLUORESCENCE MICROSCOPY; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; INFANT; MONOCYTE; PHENOTYPE; STIMULATION;

ADJUVANTS, IMMUNOLOGIC; ANTIGENS, CD3; ANTIGENS, CD34; CD4-POSITIVE T-LYMPHOCYTES; CELL DIVISION; COCULTURE TECHNIQUES; EPITHELIAL CELLS; HUMANS; INFANT; INTERLEUKIN-7; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MONOCYTES; PHENOTYPE; STEM CELLS; STIMULATION, CHEMICAL; THYMOSIN; THYMUS GLAND;

EID: 0032588116     PISSN: 01920561     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0192-0561(98)00060-5     Document Type: Article

References (29)

1. Aspinal, R. (1997). Age-related thymic atrophy in the mouse is due to a deficiency affecting rearrangement of the TCR during intrathymic T cell development. J. Immunol., 158, 3037-3045.
2. 1 antagonism of induced apoptosis of developing thymocytes requires the activation of multiple signal transduction pathways. F.A.S.E.B.J., 10, A441.
3. Baxevanis, C. N., Thanos, D., Reclos, G. J., Anastasopoulos, E., Tsokos, G. C., Papamatheakis, J., & Papmichail, M. (1992). Prothymosin alpha enhances human and murine MHC Class II surface antigen expression and messenger RNA accumulation. J. Immunol., 148, 1979-1984.
4. Cordero, O. J., Sarandeses, C. S., Lopez, J. L, Cancio, E., Reguerio, B. J., & Nogueria, M. (1991). Prothymosin alpha enhances interleukin-2 receptor expression in normal T-lymphocytes. Inter. J. Immunopharm., 13, 1059-1066.
5. Cordero, O. J., Sarandeses, C. S., & Nogueria, M. (1994). Prothymosin alpha receptors on peripheral blood mononuclear cells. F.E.B.S. Letters, 341, 23-27.
6. Eilers, M., Schirm, B., & Bishop, J. M. (1991). The myc protein activates transcription of the alpha-prothymosin gene. E.M.B.O. J., 10, 133-141.
7. Franco, F. J., Diaz, C., Barcia, M., & Freire, M. (1992). Thymosin alpha1 is a native peptide in several tissues. Biochemica et Biophysica Acta, 1120, 43-48.
8. Garaci, E., Rocchi, B., Perroni, L., D'Agostini, C., Soscia, F., Grelli, S., Mastino, A., & Favalli, C. (1994). Combination treatment with zidovudine, thymosin alphal and interferon alpha in human immunodeficiency virus infection. Inter. J. Clin. Lab. Res., 24, 23-28.
9. Godfrey, D. I., & Zlotnik, A. (1993). Controls points in early T-cell development. Immunol. Today, 14, 547-553.
10. Goldstein, A. L. (1994). Clinical applications of thymosin alpha-1. Cancer Invest., 12, 545-547.
11. Gupta, S., Aggarwal, S., & Nguyen, T. (1997). Accelerated spontaneous programmed cell death in lymphocytes in DiGeorge syndrome. J. Allergy Clin. Immunol., 99, S3.
12. Hollander, G. A., Wang, B., Nichogiannopoulou, A., Platenburg, P. P., van Ewijk, W., Burakoff, S. J., Gutierrez-Ramos, J. C., & Terhorst, G. (1995). Developmental control point in induction of thymic cortex regulated by a subpopulation of prothymocytes. Nature, 373, 350-356.
13. + Th2-like T-cell lines in allergic bronchopulmonary aspergillosis. J. Allergy Clin. Immunol., 94, 215-221.
14. - cells in postnatal allogeneic cultured thymic epithelia to evaluate immunodeficiency disorders. Stem Cells, 14, 702-713.
15. Knutsen, A. P., Wall, D., Mueller, K. R., & Bouhasin, J. D. (1996b). Abnormal in vitro thymocyte differentiation in a patient with severe combined immunodeficiency - Nezelof syndrome. J. Clin. Immunol., 16, 151-158.
16. - positive early human thymocytes: T cell receptor and cytokine receptor gene expression. Eur. J. Immunol., 25, 2471-2478.
17. Lai, S. Y., Molden, J., & Goldsmith, M. A. (1997). Shared γc subunit with the human interleukin-7 receptor complex: a molecular basis for the pathogenesis of x-lined severe combined immunodeficiency. J. Clin. Invest., 99, 169-177.
18. Low, T. L. K., & Goldstein, A. L. (1984). Thymosins: structure, function, therapeutic applications. Thymus, 6, 27-42.
19. Manrow, R. E., Sburlati, A. R., Hanover, J. A., & Berger, S. L. (1991). Nuclear targeting of prothymosin alpha. J. Biol. Chem., 266, 3916-3924.
20. Marie, D., Veljic, J., Ranin, J., & Jankovic, B. D. (1991). In vitro effect of prothymosin-alphal on humoral and cell-mediated immune responses in the young rat. Inter. J. Neuro. Science, 59, 135-142.
21. Moore, T. A., von Freed-Jeffry, U., Murray, R., & Zlotnik, A. (1996). Inhibition of γγ T cell development and early thymocyte maturation in IL-7 -/- mice. J. Immunol., 157, 2366-2373.
22. Moore, T. A., & Zlotnik, A. (1995). T-cell lineage commitment and cytokine responses of thymic progenitors. Blood, 86, 1850-1860.
23. Naylor, P. H., Oates, K. K., Coss, M. C., Erdos, M. R., Naylor, C. W., & Goldstein, A. L. (1992). Identification of immunoreactive forms of thymosin alphal in serum and supernatants by combining HPLC and RIA. Inter. J. Immunopharm., 14, 1267-1278.
24. Patel, D. D., Hale, L. P., Whichard, L. P., Radcliff, G., MacKay, C. R., & Haynes, B. F. (1995). Expression of CD44 molecules and CD44 ligands during human fetal development: expression of CD44 isoforms is developmentally regulated. Inter. Immunol., 7, 277-286.
25. + stem cells co-cultured with thymic epithelia from children with AIDS. Stem Cells, 14, 533-547.
26. Sherman, K. E., Jones, C. C., Goldstein, A. L., & Naylor, P. H. (1991). Low thymosin alpha-1 concentrations in patients chronically infected with the hepatitis B virus. Virol. Immunol., 4, 195-199.
27. Szabo, P., Ehleiter, D., Whittington, E., & Weksler, M. E. (1992). Prothymosin alpha expression occurs during G1 in proliferating B or T lymphocytes. Biochem. Biophys. Res. Comm., 185, 953-959.
28. 1 a thymic hormone? Clin. Immunol. Immunopath., 65, 195-200.
29. Weller, F. E., Shah, U., Cummings, G. D., Chretien, P. B., & Matchnick, (1992). Serum level of immunoreactive thymosin alpha 1 and thymosin beta 4 in large cohorts of healthy adults. Thymus, 19, 45-52.