Antogonistic effect of CRP end KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes

Molecular Microbiology

Volumn 26, Issue 5, 1997, Pages 1071-1082

  Nasser, William ^a   Robert Baudouy, Janine ^a   Reverchon, Sylvie ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP BINDING PROTEIN; PECTIN; POLYGALACTURONASE; RNA POLYMERASE; VIRULENCE FACTOR; ADENOSINE CYCLIC 3',5' MONOPHOSPHATE BINDING PROTEINS; ADENOSINE CYCLIC-3',5'-MONOPHOSPHATE BINDING PROTEINS; BACTERIAL DNA; BACTERIAL PROTEIN; CARRIER PROTEIN; CYCLIC AMP; DEOXYRIBONUCLEASE I; DNA BINDING PROTEIN; HYBRID PROTEIN; ISOENZYME; KDGR PROTEIN, ERWINIA CHRYSANTHEMI; PECTATE LYASE; PECTIN LYASE B; PELE PROTEIN, ERWINIA CHRYSANTHEMI; POLYSACCHARIDE LYASE; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR;

ARTICLE; CONTROLLED STUDY; DNA FOOTPRINTING; ERWINIA; GENE EXPRESSION REGULATION; NONHUMAN; PLANT CELL; POSITION; PRIORITY JOURNAL; PROMOTER REGION; REPRESSOR GENE; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; CHROMOSOME MAP; ENZYMOLOGY; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PECTOBACTERIUM CHRYSANTHEMI;

ERWINIA; ERWINIA CHRYSANTHEMI;

BACTERIAL PROTEINS; BASE SEQUENCE; CARRIER PROTEINS; CHROMOSOME MAPPING; CYCLIC AMP; CYCLIC AMP RECEPTOR PROTEIN; DEOXYRIBONUCLEASE I; DNA, BACTERIAL; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; ISOENZYMES; MOLECULAR SEQUENCE DATA; PECTOBACTERIUM CHRYSANTHEMI; POLYGALACTURONASE; POLYSACCHARIDE-LYASES; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 0030608658     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.6472020.x     Document Type: Article

References (42)

1. Allen, C., Reverchon, S., and Robert-Baudouy, J. (1989) Nucleotide sequence of the Erwinia chrysanthemi gene encoding 2-keto-3-deoxygluconate permease. Gene 83: 233-241.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1987) Current Protocols in Molecular Biology. New York: Wiley-Interscience.
3. Bachmann, B.S. (1990) Linkage map of Escherichia coli K12. edn 8. Microbiol Rev 54: 130-197.
4. Barras, F., Van Gijsegem, F., and Chatterjee, A.K. (1994) Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu Rev Phytopathol 32: 201-234.
5. Bell, A., Gaston, K., Williams, R., Chapman, K., Kolb, A., Buc, H., Minchin, S., Williams, J., and Busby, S. (1990) Mutations that alter the ability of the Escherichia coli cyclic AMP receptor protein to activate transcription. Nucleic Acids Res 18: 7243-7250.
6. Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
7. Carey, J. (1988) Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study. Proc Natl Acad Sci USA 85: 975-979.
8. Castillo, A., and Reverchon, S. (1997) Characterization of the pecT control region from Erwinia chrysanthemi strain 3937. J Bacteriol 179: 4909-4918.
9. Collmer, A., and Keen, N.T. (1986) The role of pectic enzymes in plant pathogenesis. Annu Rev Phytopathol 24: 383-409.
10. Condemine, G., and Robert-Baudouy, J. (1987) Tn5 insertion in kdgR, a regulatory gene of the polygalacturonate pathway in Erwinia chrysanthemi. FEMS Microbiol Lett 42: 39-46.
11. Condemine, G., and Robert-Baudouy, J. (1995) Synthesis and secretion of Erwinia chrysanthemi virulence factors are coregulated. Mol Plant Microbe Interact 8: 632-636.
12. Condemine, G., Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1986) Isolation of Erwinia chrysanthemi kduD mutants altered in pectin degradation. J Bacteriol 165: 937-941.
13. De Crombrugghe, B., Busby, S., and Buc, H. (1984) Cyclic AMP receptor protein: role in transcription activation. Science 224: 831-838.
14. Ebright, R.H. (1993) Transcription activation at Class I CAP-dependent promoters. Mol Microbiol 8: 797-802.
15. Expert, D., Sauvage, C., and Neilands, J.B. (1992) Negative transcriptional control of iron transport in Erwinia chrysanthemi involves an iron responsive two-factor system. Mol Microbiol 6: 2009-2017.
16. Ghosaini, L., Brown, A., and Sturtevant, J. (1988) Scanning calorimetric study of the thermal unfolding of catabolite activator protein from Escherichia coli in the absence and presence of cyclic mononucleotides. Biochemistry 27: 5257-5261.
17. Hudson, J.M., and Fried, M.G. (1990) Co-operative interactions between the catabolite gene activator protein and the lac repressor at the lactose promoter. J Mol Biol 214: 381-396.
18. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1985) Lactose catabolism in Erwinia chrysanthemi. J Bacteriol 162: 248-255.
19. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1987) Hexuronate catabolism in Erwinia chrysanthemi. J Bacteriol 169: 1223-1231.
20. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1989) Isolation of Erwinia chrysanthemi mutants altered in pectinolytic enzyme production. Mol Microbiol 3: 1587-1597.
21. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1992) Analysis of the regulation of pelBC genes in Erwinia chrysanthemi 3937. Mol Microbiol 6: 2363-2376.
22. Hugouvieux-Cotte-Pattat, N., Dominguez, H., and Robert-Baudouy, J. (1992) Environmental conditions affect the transcription of the pectinase genes of Erwinia chrysanthemi 3937. J Bacteriol 174: 7807-7818.
23. Hugouvieux-Cotte-Pattat, N., Condemine, G., Nasser, W., and Reverchon, S. (1996) Regulation of pectinolysis in Erwinia chrysanthemi. Annu Rev Microbiol 50: 213-257.
24. Kolb, A., Busby, S., Buc, H., Garges, S., and Adhya, S. (1993) Transcriptional regulation by cAMP and its receptor protein. Annu Rev Biochem 62: 749-795.
25. Kotoujansky, A. (1987) Molecular genetics of pathogenesis by soft-rot erwinias. Annu Rev Phytopathol 25: 405-430.
26. Kotoujansky, A., Lemattre, M., and Boistard, F. (1982) Utilization of thermosensitive episome bearing transposon Tn10 to isolate Hfr donor strain of Erwinia carotovora ssp. chrysanthemi. J Bacteriol 150: 122-131.
27. Laurent, F., Kotoujansky, A., Labesse, G., and Bertheau, Y. (1993) Characterization and overexpression of the pem gene encoding pectin methylesterase of Erwinia chrysanthemi strain 3937. Gene 131: 17-25.
28. Maes, M., and Messens, E. (1992) Phenol as grinding material in RNA preparations. Nucleic Acids Res 20: 4374.
29. Miller, J.H. (1972) Experiment in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
30. Nasser, W., Reverchon, S., and Robert-Baudouy, J. (1992) Purification and functional characterisation of KdgR protein, a major repressor of pectinolysis genes of Erwinia chrysanthemi. Mol Microbiol 6: 257-265.
31. Nasser, W., Condemine, G., Plantier, R., Anker, D., and Robert-Baudouy, J. (1991) Inducing properties of analogs of 2-keto-3-deoxygluconate on the expression of pectinase genes of Erwinia chrysanthemi. FEMS Microbiol Lett 81: 73-78.
32. Nasser, W., Reverchon, S., Condemine, G., and Robert-Baudouy, J. (1994) Specific interactions of Erwinia chrysanthemi KdgR repressor with different operators of genes involved in pectinolysis. J Mol Biol 236: 427-440.
33. Praillet, T., Reverchon, S., and Nasser, W. (1997) Mutual control of the PecS/PecM couple, two proteins regulating virulence-factor synthesis in Erwinia chrysanthemi. Mol Microbiol 24: 803-814.
34. Praillet, T., Nasser, W., Robert-Baudouy, J., and Reverchon, S. (1996) Purification and functional characterization of PecS: a regulator of virulence factor synthesis in Erwinia chrysanthemi. Mol Microbiol 20: 391-402.
35. Reverchon, S., Huang, Y., Bourson, C., and Robert-Baudouy, J. (1989) Nucleotide sequence of the Erwinia chrysanthemi ogl and pelE genes, negatively regulated by the kdgR product. Gene 85: 125-134.
36. Reverchon, S., Nasser, W., and Robert-Baudouy, J. (1991) Characterization of kdgR, a gene of Erwinia chrysanthemi that regulates pectin degradation. Mol Microbiol 5: 2203-2216.
37. Reverchon, S., Nasser, W., and Robert-Baudouy, J. (1994) pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi. Mol Microbiol 11: 1127-1139.
38. Reverchon, S., Expert, D., Robert-Baudouy, J., and Nasser, W. (1997) The cyclic AMP receptor protein is the main activator of the pectinolysis genes in Erwinia chrysanthemi. J Bacteriol 179: 3500-3508.
39. Shevchik, V.E., Condemine, G., Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1996) Characterization of pectin methylesterase B, an outer membrane lipoprotein of Erwinia chrysanthemi 3937. Mol Microbiol 19: 455-466.
40. Sogaard-Andersen, L., and Valentin-Hansen, P. (1993) Protein-Protein interactions in gene regulation: the cAMP-CRP complex sets the specificity of a second DNA-binding protein, the CytR repressor. Cell 75: 557-566.
41. Sogaard-Andersen, L., Pedersen, H., Holst, B., and Valentin-Hansen, P. (1991) A novel function of the cAMP-CRP complex in Escherichia coli: cAMP-CRP functions as an adaptor for the CytR repressor in the deo operon. Mol Microbiol 5: 969-975.
42. Surgey, N., Robert-Baudouy, J., and Condemine, G. (1996) The Erwinia chrysanthemi pecT gene regulates pectinase gene expression. J Bacteriol 178: 1593-1599.