Deletion of p16(INK4A)/CDKN2 and p15(INK4B) in human somatic cell hybrids and hybrid-derived tumors

Cell Growth and Differentiation

Volumn 10, Issue 1, 1999, Pages 27-33

  Kuerbitz, Steven J ^a   Malandro, Jennifer ^a   Compitello, Nicole ^a   Baylin, Stephen B ^b   Graff, Jeremy R ^b,c  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR; PROTEIN P15; PROTEIN P16; CARRIER PROTEIN; CDKN2B PROTEIN, HUMAN; CDKN2B PROTEIN, MOUSE; CELL CYCLE PROTEIN; CYCLIN DEPENDENT KINASE INHIBITOR 2B; MESSENGER RNA; PROTEIN P16INK4A; TUMOR SUPPRESSOR PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL FUSION; CELL IMMORTALIZATION; CONTROLLED STUDY; DIPLOIDY; FIBROBLAST; FIBROSARCOMA; GENE DELETION; GENE INACTIVATION; HUMAN; HUMAN CELL; HYBRID CELL; HYBRIDOMA; NONHUMAN; NUDE MOUSE; PRIORITY JOURNAL; SQUAMOUS CELL CARCINOMA; TUMOR SUPPRESSOR GENE; ANIMAL; CARCINOGEN TESTING; CELL CULTURE; CELL LINE; CHROMOSOME 9; CHROMOSOME DELETION; CYTOLOGY; GENETIC TRANSFECTION; GENETICS; MOUSE; PHYSIOLOGY;

ANIMALIA; MUS MUSCULUS;

ANIMALS; CARCINOGENICITY TESTS; CARCINOMA, SQUAMOUS CELL; CARRIER PROTEINS; CELL CYCLE PROTEINS; CELL LINE; CHROMOSOME DELETION; CHROMOSOMES, HUMAN, PAIR 9; CYCLIN-DEPENDENT KINASE INHIBITOR P15; CYCLIN-DEPENDENT KINASE INHIBITOR P16; DIPLOIDY; FIBROBLASTS; FIBROSARCOMA; GENES, TUMOR SUPPRESSOR; HUMANS; HYBRID CELLS; MICE; MICE, NUDE; RNA, MESSENGER; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEINS;

EID: 0032587374     PISSN: 10449523     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Kamb, A., Gruis, N. A., Weaver-Feldhaus, J., Liu, Q., Harshman, K., Tavtigian, S. V., Stockert, E., Day, R. S., III, Johnson, B. E., and Skolnick, M. H. A cell cycle regulator potentially involved in the genesis of many tumor types. Science (Washington DC), 264: 436-440, 1994.
2. Weaver-Feldhaus, J., Gruis, N. A., Neuhausen, S., Le Paslier, D., Stockert, E., Skolnick, M. H., and Kamb, A. Localization of a putative tumor suppressor gene by using homologous deletions in melanomas. Proc. Natl. Acad. Sci. USA, 97: 7563-7567, 1994.
3. Reed, A., Califano, J., Cairns, P., Westra, W. H., Jones, R., Koch, W., Bartek, J., and Sidransky, D. High frequency of p16 (CDKN2/MTS1/INK4A) inactivation in head and neck squamous cell carcinoma. Cancer Res., 56: 3630-3633, 1996.
4. Cairns, P., Polascik, T. J., Eby, Y., Tokino, K., Califano, J., Merlo, A., Mao, L., Herath, J., Jenkins, R., Westra, W., Rutter, J. L., Buckler, A., Gabrielson, E., Tockman, M., Cho, K. R., Hedrick, L., Bova, G. S., Isaacs, W., Koch, W., Schwab, D., and Sidransky, D. Frequency of homozygous deletion at p16/CDKN2 in primary human tumors. Nat. Genet., 11: 210-212, 1995.
5. Jen, J., Harper, W., Bigner, S., Bigner, D., Papadopoulos, N., Markowitz, S., Willson, J. K. V., Kinzler, K., and Vogelstein, B. Deletion of p16 and p15 genes in brain tumors. Cancer Res., 54: 6353-6358, 1994.
6. INK4B/MTS2 in pediatric acute lymphoblastic leukemia. Blood, 85: 2321-2330, 1995.
7. Hussussian, C. J., Struewing, J. P., Goldstein, A. M., Higgins, P. A. T., Ally, D. S., Sheahan, M. D., Clark, W. H., Jr., Tucker, M. A., and Dracopoli, N. C. Germline p16 mutations in familial melanoma. Nat. Genet., 8: 15-21, 1994.
8. Cairns, P., Mao, L., Merlo, A., Lee, D. J., Schwab, D., Eby, Y., Tokino, K., van der Riet, P., Blaugrund, J. E., and Sidransky, D. Rates of p16 (MTS1) mutations in primary tumors with 9p loss. Science (Washington DC), 265: 415-417.
9. INK4 in human cancer. Proc. Natl. Acad. Sci. USA, 97: 11045-11049, 1994.
10. Ueki, K., Rubio, M-P., Ramesh, V., Correa, K. M., Rutter, J. L., von Deimling, A., Buckler, A. J., Gusella, J. F., and Louis, D. N. MTS1/CDKN2 gene mutations are rare in primary human astrocytomas with allelic loss of chromosome 9p. Hum. Mol. Genet., 3: 1841-1845.
11. Merlo, A., Herman, J. G., Mao, L., Lee., D. J., Gabrielson, E., Burger, P. C., Baylin, S. B., and Sidransky, D. 5′ CpG island methylation is associated with transcriptional silencing of the tumor suppressor CDKN2/p16 in human cancers. Nat. Med., 7: 686-692, 1995.
12. Gonzales-Zulueta, M., Bender, C. M., Yang, A. S., Nguyen, T., Beat, R. W., Van Tornout, J. M., and Jones, P. A. Methylation of the 5′ CpG island of the p16/CDKN2 tumor suppressor gene in normal and transformed tissues correlates with gene silencing. Cancer Res., 55: 4531-4535, 1995.
13. Costello, J. F., Berger, M. S., Huang, H-J. S., and Cavanee, W. K. Silencing of p16/CDKN2 in human gliomas by methylation and chromatin condensation. Cancer Res., 56: 2405-2410, 1996.
14. Lo, K-W., Cheung, S-T., Leung, S-F., van Hasselt, A., Tsang, Y-S., Mak, K-F., Chung, Y-F., Woo, J. K. S., Lee, J. C. K., and Huang, D. P. Hypermethylation of the p16 gene in nasopharyngeal carcinoma. Cancer Res., 56: 2721-2725, 1996.
15. INK4A characterize the major types of hematological malignancies. Cancer Res., 57: 837-841, 1997.
16. INK4B. Cancer Res., 56: 722-727, 1996.
17. Sakai, T., Toguchida, J., Ohtani, N., Yandell, D. W., Rapaport, J. M., and Dryja, T. P. Allele-specific methylation of the retinoblastoma tumor suppressor gene. Am. J. Hum. Genet., 48: 880-888, 1991.
18. Herman, J. G., Latif, F., Weng, Y., Lerman, M. J., Zbar, B., Lin, S., Samid, D., Duan, D-S. R., Gnarra, J. R., Linehan, M., and Baylin, S. B. Silencing of the VHL tumor suppressor gene by DNA methylation in renal carcinoma. Proc. Natl. Acad. Sci. USA, 91: 9700-9704, 1994.
19. Kane, M. F., Massimo, L., Gaida, G. M., Lipman, J., Mishra, R., Goldman, H., Jessup, J. M., and Kolodner, R. Methylation of the hMLH1 promoter correlates with lack of expression of hMLH1 in sporadic colon tumors and mismatch repair-defective human tumor cell lines. Cancer Res., 57: 808-811, 1997.
20. Chen, P., Ellmore, N., and Weissman, B. E. Functional evidence for a second tumor suppressor gene on human chromosome 17. Mol. Cell. Biol., 14: 534-542, 1994.
21. Klingelhutz, A. J., Wu, S-Q., Huang, J., and Reznikoff, C. A. Loss of 3p13-p21.2 in tumorigenic reversion of a hybrid between isogeneic non-tumorigenic and tumorigenic human uroepithelial cells. Cancer Res., 52: 1631-1634, 1992.
22. Benedict, W. F., Weissman, B. E., and Stanbridge, E. J. Tumorigenicity of HT1080 fibrosarcoma × normal fibroblast hybrids: chromosome dosage dependency. Cancer Res., 44: 3471-3479, 1984.
23. INK4A transcript. Cancer Res., 55: 2995-2997, 1995.
24. Stone, S. Jiang, P., Dayananth, P., Tavtigian, S., Katcher, H., Parry, D., Peters, G., and Kamb, A. Complex structure and regulation of the p16 (MTS1) locus. Cancer Res., 55: 2988-2994, 1995.
25. Liggett, W. H., Jr., Sewell, D. A., Rocco, J., Ahrendt, S. A., Koch, W., and Sidransky, D. p16 and p15 are potent growth suppressors of head and neck squamous carcinoma cells in vitro. Cancer Res., 56: 4119-4123, 1996.
26. Kuerbitz, S. J., and Baylin, S. B. Retention of unmethylated CpG island alleles in human diploid fibroblast × fibrosarcoma cell lines expressing high levels of DNA methyltransferase. Cell Growth Differ., 7: 847-853, 1996.
27. Tlsty, T. D., White, A., and Sanchez, J. Suppression of gene amplification in human cell hybrids. Science (Washington DC), 255: 1425-1427, 1992.
28. Arap, W., Nishikawa, R., Furnari, F. B., Cavanee, W. K., and Huang, H. J. Replacement of the p16/CDKN2 gene suppresses human glioma cell growth. Cancer Res., 55: 1351-1354, 1995.
29. Stone, S., Dayananth, P., Jiang, P., Weaver-Feldhaus, J. M., Tavtigian, S. V., Cannon-Albright, L., and Kamb, A. Genomic structure, expression and mutational analysis of the p15 (MTS2) gene. Oncogene, 11: 987-991, 1995.
30. Serrano, M., Lee, W-H., Chin, L., Cordon-Cardo, C., Beach, D., and DePinho, R. A. Role of the INK4a locus in tumor suppression and cell mortality. Cell, 85: 27-37, 1996.
31. Kamijo, T, Zindy, F, Roussel, M. F., Quella, D. E., Downing, J. R., Ashmun, R. A., Grosveld, G., and Sherr, C. J. Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product. p19ARF. Cell, 91: 649-659, 1997.
32. Nielsen, G. P., Burns, K. L., Rosenberg, A. E., and Louis, D. N. CDKN2A gene deletions and loss of p16 expression occur in osteosarcomas that lack RB alterations. Am J. Pathol., 153: 159-163, 1998.
33. Chomczynski, P., and Sacchi, N. Single step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162: 156-159, 1987.