N5-(L-1-carboxyethyl)-L-ornithine synthase: Physical and spectral characterization of the enzyme and its unusual low pK(a) fluorescent tyrosine residues

Protein Science

Volumn 8, Issue 10, 1999, Pages 2121-2129

  Sackett, Dan L ^a,d   Ruvinov, Sergei B ^b   Thompson, John ^c  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^c NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH   (United States)

^d NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

N5 (L 1 carboxyethyl)ornithine synthase; Proteolysis; Tryptophan fluorescence; Tyrosine; Tyrosine pK(a)

Indexed keywords

CHYMOTRYPSIN; N5 (1 CARBOXYETHYL)ORNITHINE SYNTHETASE; SYNTHETASE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG; N(5) (CARBOXYETHYL)ORNITHINE SYNTHASE; N(5)-(CARBOXYETHYL)ORNITHINE SYNTHASE; OXIDOREDUCTASE; RECOMBINANT PROTEIN;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; MOLECULAR WEIGHT; PKA; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SPECTROFLUOROMETRY; STRUCTURE ANALYSIS; AMINO ACID SEQUENCE; CHEMISTRY; ENZYMOLOGY; LACTOCOCCUS LACTIS; PH;

ESCHERICHIA COLI; LACTOCOCCUS LACTIS;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; CHYMOTRYPSIN; HYDROGEN-ION CONCENTRATION; LACTOCOCCUS LACTIS; MOLECULAR WEIGHT; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; TRYPTOPHAN; TYROSINE;

EID: 0032585920     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.10.2121     Document Type: Article

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