Distinct compartmentalization of TGN46 and β1,4-galactosyltransferase in HeLa cells

European Journal of Cell Biology

Volumn 72, Issue 3, 1997, Pages 238-246

  Prescott, Alan R ^a   Lucocq, John M ^a   James, John ^a   Lister, Jane M ^a   Ponnambalam, Sreenivasan ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Golgi apparatus; TGN38; TGN46; Trans Golgi network (TGN); Tubulovesicular

Indexed keywords

CELL PROTEIN; GALACTOSYLTRANSFERASE; GLYCOSYLATED PROTEIN; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ARTICLE; CELLULAR DISTRIBUTION; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME LOCALIZATION; FLUORESCENCE MICROSCOPY; GOLGI COMPLEX; HELA CELL; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN LOCALIZATION;

EID: 0030889062     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Allen, H. J., E. C. Kisailus: Glycoconjugates: Composition, Structure and Function. Marcel Dekker Inc. New York 1992.
2. Amano, J., P. Straehl, E. G. Berger, N. Kochibe, A. Kobata: Structures of mucin-type sugar chains of the galactosyltransferase purified from human milk. Occurrence of the ABO and Lewis blood group determinants. J. Biol. Chem. 266, 11461-11477 (1991).
3. Berger, E. G., P. Burger, A. Hille, T. Bächi: Comparative localization of mannose-6-phosphate receptor with 2,6sialyltransferase in HepG2 cells: an analysis by confocal double immunofluorescence microscopy. Eur. J. Cell Biol. 67, 106-111 (1995).
4. Berger, E. G., K. Grimm, T. Bachi, H. Bosshart, R. Kleene, M. Watzele: Double immunofluorescent staining of α2,6 sialyltransferase and β1,4 galactosyltransferase in monensin-treated cells, evidence for different Golgi subcompartments? J. Cell. Biochem. 52, 275-288 (1993).
5. Bos, K., C. Wraight, K. Stanley: Tgn38 is maintained in the trans-Golgi network by tyrosine-containing motif in the cytoplasmic domain. EMBO J. 12, 2219-2228 (1993).
6. Griffiths, G., S. D. Fuller, R. Back, M. Hollinshead, S. Pfeiffer, K. Simons: The dynamic nature of the Golgi complex. J. Cell Biol. 108, 277-297 (1989).
7. Griffiths, G., R. G. Parton, J. Lucocq, B. van Deurs, D. Brown, J. W. Slot, H. J. Geuze: The immunofluorescent era of membrane traffic. Trends Cell Biol. 3, 214-219 (1993).
8. Griffiths, G., R. Pepperkok, J. Krinjse-Locker, T. Kreis: Immunocytochemical localization of β-COP to the ER-Golgi boundary and the TGN. J. Cell Sci. 108, 2839-2856 (1995).
9. Griffiths, G., K. Simons: The trans-Golgi network: sorting at the exit site of the Golgi complex. Science 234, 438-443 (1986).
10. Griffiths, G., G. Warren, P. Quinn, C. O. Mathieu, H. Hoppeler: Density of newly synthesized plasma membrane proteins in intracellular membranes. I. Stereological studies. J. Cell Biol. 98, 2133-2141 (1984).
11. Harlow, E., D. Lane: Antibodies. A Laboratory Manual. Cold Spring Harbor Laboratory Press. New York 1988.
12. Humphrey, J. S., P. J. Peters, L. C. Yuan, J. S. Bonifacino: A tyrosine-containing sequence within the cytoplasmic domain of TGN38 confers localization to the trans-Golgi network. J. Cell Biol. 120, 1123-1135 (1993).
13. Jones, S. M., J. R. Crosby, J. Salamero, K. E. Howell: A cytosolic complex of p62 and rab6 associates with TGN38/41 and is involved in budding of exocytic vesicles from the trans-Golgi network. J. Cell Biol. 122, 775-788 (1993).
14. Kawano, J. I., S. Ide, T. Oinuma, T. Suganuma: A protein-specific monoclonal antibody to rat liver β1,4-galactosyltransferase and its application to immunohistochemistry. J. Histochem. Cytochem. 42, 363-369 (1994).
15. Kornfeld, S.: Structure and function of the mannose 6-phosphate insulin-like growth factor II receptors. Annu. Rev. Biochem. 61, 307-330 (1992).
16. Kornfeld, R., S. Kornfeld: Assembly and synthesis of asparagine-linked oligosaccharides. Annu. Rev. Biochcm. 54, 631-664 (1985).
17. Lucocq, J. M.: Quantitation of gold labeling and estimation of labeling efficiency with a stereological counting method. J. Histochem. Cytochem. 40, 1929-1936 (1992).
18. Lucocq, J. M., E. Berger, C. Hug: The pathway of Golgi cluster formation in okadaic acid-treated cells. J. Struct. Biol. 115, 318-330 (1995).
19. Luzio, J. P., B. Brake, G. Banting, K. E. Howell, P. Braghetta, K. K. Stanley: Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38). Biochem. J. 270, 97-102 (1990).
20. Swift, A. M., C. E. Machamer: A Golgi retention signal in the membrane-spanning domain of the coronavirus E1 protein. J. Cell Biol. 115, 19-30 (1991).
21. Mellman, I., K. Simons: The Golgi complex: in vitro veritas? Cell 68, 829-840 (1992).
22. Munro, S.: Sequences within and adjacent to the transmembrane segment of α-2,6-sialyltransferase specify Golgi retention. EMBO J. 10, 3577-3588 (1991).
23. Nilsson, T., M. H. Hoe, C. Rabouille, P. Slusarewicz, R. Watson, G. Watzele, E. G. Berger, G. Warren: Kin recognition between medial Golgi enzymes. EMBO J. 13, 562-574 (1994).
24. Nilsson, T. J. M. Lucocq, D. Mackay, G. Warren: The membrane spanning domain of β-1,4-galactosyltransferase specifies trans-Golgi localization. EMBO J. 10, 3567-3575 (1991).
25. Nilsson, T., M. Pypaert, M. H. Hoe, P. Slusarewicz, E. G. Berger, G. Warren: Overlapping distribution of two glycosyltransferases in the Golgi apparatus of HeLa cells. J. Cell Biol. 120, 5-13 (1993).
26. Nilsson, T., G. Warren: Retention and retrieval in the endoplasmic reticulum and Golgi apparatus. Curr. Opin. Cell Biol. 6, 517-521 (1994).
27. Ponnambalam, S., M. Girotti, M.-L. Yaspo, C. E. Owen, A. C. F. Perry, T. Suganuma, T. Nilsson, M. Fried, G. Banting, G. Warren: Primate homologues of rat TGN38: structure, expression and functional implications. J. Cell Sci. 109, 675-685 (1996).
28. Ponnambalam, S., C. Rabouille, J. P. Luzio, T. Nilsson, G. Warren: The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network. J. Cell Biol. 125, 253-268 (1994).
29. Rabouille, C., N. H. Hui, R. Watson, F. Hunte, E. Berger, G. Warren, T. Nilsson: Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J. Cell Sci. 108, 1617-1627 (1995).
30. Reaves, B., G. Banting: Over-expression of TGN38/41 leads to mis-localisation of γ-adaptin. FEBS Lett. 351, 448-456 (1994).
31. Roth, J., E. G. Berger: Immunocytochemical localization of galactosyltransferase in HeLa cells, co-distribution with thiamine pyrophosphatase in trans-Golgi cisternae. J. Cell Biol. 92, 223-229 (1982).
32. Sambrook, J., E. F. Fritsch, T. Maniatis: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. New York 1989.
33. Schachter, H.: Biosynthesis of N- and O-glycans. In: Tools for Glycobiology. pp. 9-11. Oxford Glycosystems. Oxford/UK 1994.
34. Slusarewicz, P., T. Nilsson, N. Hui, R. Watson, G. Warren: Isolation of a matrix that binds medial Golgi enzymes. J. Cell Biol. 124, 405-413 (1994).
35. Strous, G. J., P. Van Kerkhof, R. Willemsen, H. J. Geuze, E. G. Berger: Transport and topology of galactosyltransferase in endomembranes of HeLa cells. J. Cell Biol. 97, 723-727 (1983).
36. Velasco, A., L. Hendricks, K. W. Moremen, D. R. Tulsiani, O. Touster, M. G. Farquhar: Cell type-dependent variations in the subcellular distribution of α-mannosidase I. J. Cell Biol. 122, 39-51 (1993).
37. Watzele, G., R. Bachofner, E. G. Berger: Immunocytochemical localization of the Golgi apparatus using protein-specific antibodies to galactosyltransferase. Eur. J. Cell Biol. 56, 451-458 (1991).
38. Weidmann, P.: Anterograde transport through the Golgi complex: do Golgi tubules hold the key? Trends Cell Biol. 5, 302-305 (1995).
39. Weidmann, P., R. Roth, J. Heuser: Golgi membrane dynamics imaged by freeze-etch electron microscopy: views of different membrane coatings involved in tubulation versus vesiculation. Cell 75, 123-133 (1993).
40. Wong, S. H., W. Wong: The SXYQRL sequence in the cytoplasmic domain of TGN38 plays a major role in trans-Golgi network localization. J. Biol. Chem. 268, 22853-22862 (1993).